An intraspecific linkage map of the chickpea (Cicer arietinum L.) genome based on sequence tagged microsatellite site and resistance gene analog markers

Ascochyta blight, caused by the fungus Ascochyta rabiei (Pass.) Lab., is one of the most devastating diseases of chickpea ( Cicer arietinum L.) worldwide. Research was conducted to map genetic factors for resistance to ascochyta blight using a linkage map constructed with 144 simple sequence repeat markers and 1 morphological marker (fc, flower colour). Stem cutting was used to vegetatively propagate 186 F2 plants derived from a cross between Cicer arietinum L. ‘ICCV96029’ and ‘CDC Frontier’. A total of 556 cutting-derived plants were evaluated for their reaction to ascochyta blight under controlled conditions. Disease reaction of the F1 and F2 plants demonstrated that the resistance was dominantly inherited. A Fain’s test based on the means and variances of the ascochyta blight reaction of the F3 families showed that a few genes were segregating in the population. Composite interval mapping identified 3 genomic regions that were associated with the reaction to ascochyta blight. One quantitative trait locus (QTL) on each of LG3, LG4, and LG6 accounted for 13%, 29%, and 12%, respectively, of the total estimated phenotypic variation for the reaction to ascochyta blight. Together, these loci controlled 56% of the total estimated phenotypic variation. The QTL on LG4 and LG6 were in common with the previously reported QTL for ascochyta blight resistance, whereas the QTL on LG3 was unique to the current population.

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A linkage map of chickpea (Cicer arietinum L.) based on populations from Kabuli × Desi crosses: location of genes for resistance to fusarium wilt race 0

Theoretical and Applied Genetics ◽

10.1007/s00122-005-1980-1 ◽

2005 ◽

Vol 110 (7) ◽

pp. 1347-1353 ◽

Cited By ~ 75

Author(s):

M. J. Cobos ◽

M. J. Fernández ◽

J. Rubio ◽

M. Kharrat ◽

M. T. Moreno ◽

...

Keyword(s):

Linkage Map ◽

Cicer Arietinum ◽

Fusarium Wilt ◽

Cicer Arietinum L

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High-Throughput SNP Discovery and Genotyping for Constructing a Saturated Linkage Map of Chickpea (Cicer arietinum L.)

DNA Research ◽

10.1093/dnares/dss018 ◽

2012 ◽

Vol 19 (5) ◽

pp. 357-373 ◽

Cited By ~ 95

Author(s):

R. Gaur ◽

S. Azam ◽

G. Jeena ◽

A. W. Khan ◽

S. Choudhary ◽

...

Keyword(s):

Linkage Map ◽

Cicer Arietinum ◽

High Throughput ◽

Snp Discovery ◽

Cicer Arietinum L

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Construction of a Linkage Map and Identification of Resistance Gene Analog Markers for Root-knot Nematodes in Wild Peach, Prunus kansuensis

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.136.3.190 ◽

2011 ◽

Vol 136 (3) ◽

pp. 190-197 ◽

Cited By ~ 11

Author(s):

Ke Cao ◽

Lirong Wang ◽

Gengrui Zhu ◽

Weichao Fang ◽

Chenwen Chen ◽

...

Keyword(s):

Linkage Map ◽

Resistance Gene ◽

Dna Sequences ◽

Open Reading Frames ◽

Resistance Gene Analog ◽

Root Knot Nematode ◽

Degenerate Primers ◽

Morphological Marker ◽

Backcross Population ◽

Peach Genome

The root-knot nematode (RKN) Meloidogyne incognita can cause severe crop loss in economically important Prunus species like peach (P. persica), almond (P. communis), plum (P. salicina), and apricot (P. armeniaca). Some peach rootstock, including Nemaguard (P. persica), Nemared (P. persica), and Myrobalan plum (P. cerasifera), display significant resistance to RKN. We present a genetic linkage map constructed by using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) for a peach backcross population (190 individuals) of RKN-resistant ‘Honggengansutao’ (P. kansuensis) and susceptible ‘Bailey’ (P. persica). Degenerate primers designed from conserved motifs of known plant resistance gene (R) products were used to amplify genomic DNA sequences. Twenty-two resistance gene analog (RGA) sequences were selected from 48 RGAs with open-reading frames to design sequence-tagged site markers. The linkage map of ‘Honggengansutao’ is composed of 138 loci (30 SSRs, 102 SRAPs, five RGAs, and one morphological marker for RKN resistance) assigned to eight linkage groups. The map covers 616 cM of the peach genome with an average marker spacing of 4.9 cM. The five RGAs were mapped to Groups 2, 7, and 8. One gene (designated PkMi) involved in resistance to RKN was mapped to Group 2 (which also includes the known RKN-resistance RMia gene). BLASTN analysis mapped all RGAs to the peach genome sequence. The map constructed in the study will aid future rootstock breeding with marker-assisted selection to identify additional candidate RGA sequences.

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