scholarly journals Construction of a Linkage Map and Identification of Resistance Gene Analog Markers for Root-knot Nematodes in Wild Peach, Prunus kansuensis

2011 ◽  
Vol 136 (3) ◽  
pp. 190-197 ◽  
Author(s):  
Ke Cao ◽  
Lirong Wang ◽  
Gengrui Zhu ◽  
Weichao Fang ◽  
Chenwen Chen ◽  
...  
Keyword(s):  
Linkage Map ◽  
Resistance Gene ◽  
Dna Sequences ◽  
Open Reading Frames ◽  
Resistance Gene Analog ◽  
Root Knot Nematode ◽  
Degenerate Primers ◽  
Morphological Marker ◽  
Backcross Population ◽  
Peach Genome

The root-knot nematode (RKN) Meloidogyne incognita can cause severe crop loss in economically important Prunus species like peach (P. persica), almond (P. communis), plum (P. salicina), and apricot (P. armeniaca). Some peach rootstock, including Nemaguard (P. persica), Nemared (P. persica), and Myrobalan plum (P. cerasifera), display significant resistance to RKN. We present a genetic linkage map constructed by using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) for a peach backcross population (190 individuals) of RKN-resistant ‘Honggengansutao’ (P. kansuensis) and susceptible ‘Bailey’ (P. persica). Degenerate primers designed from conserved motifs of known plant resistance gene (R) products were used to amplify genomic DNA sequences. Twenty-two resistance gene analog (RGA) sequences were selected from 48 RGAs with open-reading frames to design sequence-tagged site markers. The linkage map of ‘Honggengansutao’ is composed of 138 loci (30 SSRs, 102 SRAPs, five RGAs, and one morphological marker for RKN resistance) assigned to eight linkage groups. The map covers 616 cM of the peach genome with an average marker spacing of 4.9 cM. The five RGAs were mapped to Groups 2, 7, and 8. One gene (designated PkMi) involved in resistance to RKN was mapped to Group 2 (which also includes the known RKN-resistance RMia gene). BLASTN analysis mapped all RGAs to the peach genome sequence. The map constructed in the study will aid future rootstock breeding with marker-assisted selection to identify additional candidate RGA sequences.

scholarly journals Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

2017 ◽  
Vol 1 (2) ◽  
pp. 200-201
Author(s):  
Eugeny Nikolaevich Sysoliatin ◽  
Natalia Vladimirovna Anisimova ◽  
Olga Gennadievna Babak
Keyword(s):  
Molecular Markers ◽  
Genetic Polymorphism ◽  
Resistance Gene ◽  
Lupinus Angustifolius ◽  
Resistance Gene Analog ◽  
Degenerate Primers

Abstract We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.

Temporal Changes in Resistance Gene Analog Polymorphism of Major Rice Varieties in Guangdong Province

2013 ◽  
Vol 39 (7) ◽  
pp. 1206
Author(s):  
Zhi-Ming TANG ◽  
Jian-You CHEN ◽  
Yong-Fa LU ◽  
Hua-Cheng ZHOU ◽  
Xue-Jiao MO ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Guangdong Province ◽  
Temporal Changes ◽  
Resistance Gene Analog ◽  
Rice Varieties

scholarly journals Inheritance and linkage analysis of co-dominant SSR markers on the Z chromosome of the silkworm (Bombyx mori L.)

2008 ◽  
Vol 90 (2) ◽  
pp. 151-156 ◽  
Author(s):  
XUE-XIA MIAO ◽  
WEI-HUA LI ◽  
MU-WANG LI ◽  
YUN-PO ZHAO ◽  
XIAN-RU GUO ◽  
...  
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Ssr Markers ◽  
Positional Cloning ◽  
Z Chromosome ◽  
Pcr Marker ◽  
Backcross Population ◽  
In The Beginning ◽  
Simple Sequence ◽  
Visible Mutation

SummaryMicrosatellites or simple sequence repeats (SSRs) are co-dominant molecular markers. When we used fluorescent SSR markers to construct a linkage map for the female heterogametic silkworm (Bombyx mori, ZW), we found that some loci did not segregate in a Mendelian ratio of 1:1 in a backcross population. These loci segregated in a 3:1 ratio of single bands compared with double bands. Further examination of band patterns indicated that three types of SSR bands were present: two homozygotes and one heterozygote. In the beginning, we considered to discard these markers. By scoring male and female F1 individuals, we confirmed that these loci were located on the Z chromosome. Using the sex-linked visible mutation sch (K05) and its wild-type (C108), we constructed an F1 male backcross (BC1M) mapping population. The combination of sch backcross and SSR data enabled us to map the SSR markers to the Z chromosome. By adjusting input parameters based on these data, we were able to use Mapmaker software to construct a linkage map. This strategy takes advantage of co-dominant markers for positional cloning of genes on the Z chromosome. We localized sch to the Z chromosome relative to six SSR markers and one PCR marker, covering a total of 76·1 cM. The sch mutation is an important sex-linked visible mutation widely used in breeding of commercial silkworms (e.g. male silkworm selection rearing). Localization of the sch gene may prove helpful in cloning the gene and developing strains for marker-assisted selection in silkworm breeding.

scholarly journals A Linkage-Based Genome Assembly for the Mosquito Aedes albopictus and Identification of Chromosomal Regions Affecting Diapause

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 167
Author(s):  
John H. Boyle ◽  
Pasi M. A. Rastas ◽  
Xin Huang ◽  
Austin G. Garner ◽  
Indra Vythilingam ◽  
...  
Keyword(s):  
Linkage Map ◽  
Aedes Albopictus ◽  
Dna Sequences ◽  
Genome Assembly ◽  
Single Copy ◽  
Health Concern ◽  
Candidate Snps ◽  
Single Location ◽  
Asian Tiger ◽  
Photoperiodic Diapause

The Asian tiger mosquito, Aedes albopictus, is an invasive vector mosquito of substantial public health concern. The large genome size (~1.19–1.28 Gb by cytofluorometric estimates), comprised of ~68% repetitive DNA sequences, has made it difficult to produce a high-quality genome assembly for this species. We constructed a high-density linkage map for Ae. albopictus based on 111,328 informative SNPs obtained by RNAseq. We then performed a linkage-map anchored reassembly of AalbF2, the genome assembly produced by Palatini et al. (2020). Our reassembled genome sequence, AalbF3, represents several improvements relative to AalbF2. First, the size of the AalbF3 assembly is 1.45 Gb, almost half the size of AalbF2. Furthermore, relative to AalbF2, AalbF3 contains a higher proportion of complete and single-copy BUSCO genes (84.3%) and a higher proportion of aligned RNAseq reads that map concordantly to a single location of the genome (46%). We demonstrate the utility of AalbF3 by using it as a reference for a bulk-segregant-based comparative genomics analysis that identifies chromosomal regions with clusters of candidate SNPs putatively associated with photoperiodic diapause, a crucial ecological adaptation underpinning the rapid range expansion and climatic adaptation of A. albopictus.

scholarly journals Histological Characterization of Resistance to Different Root-Knot Nematode Species Related to Phenolics Accumulation in Capsicum annuum

2005 ◽  
Vol 95 (2) ◽  
pp. 158-165 ◽  
Author(s):  
A. Pegard ◽  
G. Brizzard ◽  
A. Fazari ◽  
O. Soucaze ◽  
P. Abad ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Capsicum Annuum ◽  
High Performance ◽  
Nematode Species ◽  
Susceptible Cultivar ◽  
Resistant Line ◽  
Root Knot Nematode ◽  
Resistance Response ◽  
Root Knot Nematodes ◽  
Chromatography Analysis

In the pepper Capsicum annuum CM334, which is used by breeders as a source of resistance to Phytophthora spp. and potyviruses, a resistance gene entirely suppresses reproduction of the root-knot nematode (Meloidogyne spp.). The current study compared the histological responses of this resistant line and a susceptible cultivar to infection with the three most damaging root-knot nematodes: M. arenaria, M. incognita, or M. javanica. Resistance of CM334 to root-knot nematodes was associated with unidentified factors that limited nematode penetration and with post-penetration biochemical responses, including the hypersensitive response, which apparently blocked nematode migration and thereby prevented juvenile development and reproduction. High-performance liquid chromatography analysis suggested that phenolic compounds, especially chlorogenic acid, may be involved in CM334 resistance. The response to infection in the resistant line varied with root-knot nematode species and was correlated with nematode behavior and pathogenicity in the susceptible cultivar: nematode species that quickly reached the vascular cylinder and initiated feeding sites in the susceptible cultivar were quickly recognized in CM334 and stopped in the epidermis or cortex. After comparing our data with those from other resistant pepper lines, we suggest that timing of the resistance response and the mechanism of resistance vary with plant genotype, resistance gene, and root-knot nematode species.

Localization of Ds-transposon containing T-DNA inserts in the diploid transgenic potato: linkage to the R1 resistance gene against Phytophthora infestans (Mont.) de Bary

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 249-257 ◽  
Author(s):  
A. El-Kharbotly ◽  
J. M. E. Jacobs ◽  
B. te Lintel Hekkert ◽  
W. J. Stiekema ◽  
A. Pereira ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Resistance Gene ◽  
Dna Sequences ◽  
Transgenic Potato ◽  
Potato Genome ◽  
Rflp Map ◽  
Polymerase Chain ◽  
R1 Gene ◽  
Map Location

The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reaction or plasmid rescue techniques and used as probes for RFLP linkage analysis. The RFLP map location of 60 T-DNAs carrying Ds–NPTII was determined. The T-DNA distribution per chromosome and the relative distance between them appeared to be random. All 12 chromosomes have been covered with Ds-containing T-DNAs, potentially enabling tagging of any gene in the potato genome. The T-DNA insertions of two transformants, BET92-Ds-A16-259 and BET92-Ds-A16-416, were linked in repulsion to the position of the resistance gene R1 against Phytophthora infestans. After crossing BET92-Ds-A16-416 with a susceptible parent, 4 desired recombinants (Ds carrying T-DNA linked in coupling phase with the R1 gene) were discovered. These will be used for tagging the R1 gene. The efficiency of the pathway from the introduction to localization of T-DNAs is discussed. Key words : Solanum tuberosum, Phytophthora infestans, Ds element, transposon tagging, R genes, euchromatin.

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