Development and characterization of wheat- Leymus racemosus translocation lines with resistance to Fusarium Head Blight

Abstract Background Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Results Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal chromosomal translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian translocations and one 3D/?St double terminal translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Conclusions Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

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Molecular Cytogenetic Characterization of Wheat–Thinopyrum elongatum Addition, Substitution and Translocation Lines with a Novel Source of Resistance to Wheat Fusarium Head Blight

Journal of Genetics and Genomics ◽

10.1016/j.jgg.2011.11.008 ◽

2012 ◽

Vol 39 (2) ◽

pp. 103-110 ◽

Cited By ~ 52

Author(s):

Shulan Fu ◽

Zhenling Lv ◽

Bao Qi ◽

Xiang Guo ◽

Jun Li ◽

...

Keyword(s):

Fusarium Head Blight ◽

Head Blight ◽

Molecular Cytogenetic ◽

Cytogenetic Characterization ◽

Translocation Lines ◽

Thinopyrum Elongatum

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Characterization of Fusarium Head Blight Resistance Gene Fhb1 and Its Putative Ancestor in Chinese Wheat Germplasm

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2018.00473 ◽

2018 ◽

Vol 44 (4) ◽

pp. 473 ◽

Cited By ~ 10

Author(s):

Zhan-Wang ZHU ◽

Deng-An XU ◽

Shun-He CHENG ◽

Chun-Bao GAO ◽

Xian-Chun XIA ◽

...

Keyword(s):

Fusarium Head Blight ◽

Resistance Gene ◽

Fusarium Head Blight Resistance ◽

Blight Resistance ◽

Head Blight ◽

Wheat Germplasm ◽

Chinese Wheat

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Establishment and characterization of a complete set of Triticum durum-Thinopyrum elongatum monosomic addition lines with resistance to Fusarium head blight in wheat

Journal of Genetics and Genomics ◽

10.1016/j.jgg.2019.09.006 ◽

2019 ◽

Vol 46 (11) ◽

pp. 547-549

Author(s):

Jing Wang ◽

Qinghua Shi ◽

Xianrui Guo ◽

Fangpu Han

Keyword(s):

Fusarium Head Blight ◽

Triticum Durum ◽

Addition Lines ◽

Head Blight ◽

Monosomic Addition ◽

Complete Set ◽

Monosomic Addition Lines ◽

Thinopyrum Elongatum

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Molecular cytogenetic characterization of four partial wheat-Thinopyrum ponticum amphiploids and their reactions to Fusarium head blight, tan spot, and Stagonospora nodorum blotch

Theoretical and Applied Genetics ◽

10.1007/s00122-006-0250-1 ◽

2006 ◽

Vol 112 (8) ◽

pp. 1473-1479 ◽

Cited By ~ 24

Author(s):

R. E. Oliver ◽

S. S. Xu ◽

R. W. Stack ◽

T. L. Friesen ◽

Y. Jin ◽

...

Keyword(s):

Fusarium Head Blight ◽

Tan Spot ◽

Stagonospora Nodorum ◽

Head Blight ◽

Stagonospora Nodorum Blotch ◽

Thinopyrum Ponticum ◽

Molecular Cytogenetic ◽

Cytogenetic Characterization

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Alien chromatin but not Fhb7 confers Fusarium head blight resistance in wheat breeding

10.1101/2021.02.03.429547 ◽

2021 ◽

Author(s):

Xianrui Guo ◽

Qinghua Shi ◽

Jing Yuan ◽

Mian Wang ◽

Jing Wang ◽

...

Keyword(s):

Fusarium Head Blight ◽

Fusarium Head Blight Resistance ◽

Resistance Breeding ◽

Wheat Breeding ◽

Glutathione S Transferase ◽

Head Blight ◽

Fhb Resistance ◽

Translocation Lines ◽

High Level ◽

Thinopyrum Elongatum

AbstractFusarium head blight (FHB), caused by Fusarium species, seriously threaten global wheat production. Three wheat-Th.elongatum FHB resistant translocation lines have been developed and used for breeding. Transcriptomic analysis identified a derivative glutathione S-transferase transcript T26102, which was homologous to Fhb7 and induced dramatically by Fusarium graminearum. Homologs of Fhb7 were detected in several genera in Triticeae, including Thinopyrum, Elymus, Leymus, Pseudoroegeria and Roegeria. Several wheat-Thinopyrum translocation lines carrying Fhb7 remain susceptible to FHB, and transgenic plants overexpressing the T26102 on different backgrounds did not improve the FHB resistance. Taken as a whole, we show the application of the chromatin derived from diploid Thinopyrum elongatum successfully conferring wheat with high level FHB resistance independent of the Fhb7.One Sentence SummaryThinopyrum elongatum chromatin from 7EL was successfully applied to wheat FHB resistance breeding, but the resistant gene other than the reported Fhb7 remained unknown.

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