scholarly journals Cytoplasmic diversity of Brassica napus L., Brassica oleracea L. and Brassica rapa L. as determined by chloroplast microsatellite markers

2012 ◽  
Vol 60 (3) ◽  
pp. 953-965 ◽  
Author(s):  
Shirin Zamani-Nour ◽  
Rosemarie Clemens ◽  
Christian Möllers
Keyword(s):  
Brassica Napus ◽  
Microsatellite Markers ◽  
Brassica Oleracea ◽  
Brassica Rapa ◽  
Brassica Napus L ◽  
Chloroplast Microsatellite ◽  
Cytoplasmic Diversity ◽  
Brassica Oleracea L

scholarly journals ADUBAÇÃO FOLIAR DE BORO EM DOIS HÍBRIDOS DE CANOLA

Nativa ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 600-604
Author(s):  
Anderson Bergamasco Hryczyna ◽  
Tiago Roque Benetoli da Silva ◽  
Lucas Ambrosano ◽  
Gesmila Karoline Zampronio
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Brassica Rapa ◽  
Pollen Grain ◽  
Brassica Napus L ◽  
Cell Wall Development ◽  
Leaf Boron ◽  
Boron Fertilization ◽  
Brassicaceae Family ◽  
Brassica Oleracea L

A canola (Brassica napus L. var oleífera) é uma oleaginosa, pertencente à família Brassicaceae, resultado do cruzamento interespecífico entre repolho silvestre (Brassica oleracea L.) e mostarda (Brassica rapa L., syn. campestris). O boro é considerado um micronutriente, que atua na translocação de açúcares, formação de proteínas, crescimento do tubo polínico, germinação dos grãos de pólen, desenvolvimento das sementes e das paredes celulares. O objetivo desta pesquisa foi estudar as interferências nos componentes produtivos de dois híbridos de canola com a aplicação de adubação com boro por via foliar. Para tanto, instalou-se um experimento em condições de campo num delineamento experimental de blocos ao acaso em arranjo fatorial 2 x 3. Utilizou-se os híbridos Hyola 433 e Hyola 575 CL e três doses de boro foliar (0;100; 200) g ha-1 de B. As seguintes características foram avaliadas:  número de plantas, número de síliquas por planta, massa de parte aérea, massa de 100 grãos e produtividade. Com a aplicação de boro houve aumento da massa de grãos independente do híbrido. Para produtividade houve aumento somente para o híbrido Hyola 433, sendo a maior produtividade alcançada na dose de 200 g ha-1. O híbrido Hyola 575 CL não teve sua produtividade alterada com a aplicação de boro. Palavras-chave: ácido bórico; Brassica napus L. var. oleífera; colza; massa de 100 grãos; oleaginosa.   Leaf boron fertilization in two rapessed   ABSTRACT: Rapessed (Brassica napus L. var oleífera) is an oilseed, belongs to Brassicaceae family, resultated between inter specific crossing of wild cabbage (Brassica oleracea L.) and mustard (Brassica rapa L., syn. campestris). Boron is considered a micronutrient, acting in sugars translocation, protein formation, pollen tube growth, pollen grain germination, seeds and cell wall development. The aimed was to study the interferences in productive components of two rapessed hybrids with application of boron fertilization by leaf application. For this, a field experiment was installed by using experimental design of randomized blocks in a 2 x 3 factorial scheme.  Hyola 433 and Hyola 575CL hybrids and three doses of leaf boron (0; 100 and 200) g ha-1 of B were used. The following characteristics were evaluated: plants number, number of pods per plant, mass of one hundred grains and yield. With boron application there was an increase in grain mass independent of the hybrid. In relation grain yield there was increase only for Hyola 433 hybrid, with the highest yield reached at 200 g ha-1. The Hyola 575CL hybrid did not have its productivity altered with the application of boron. Keywords: boric acid; Brassica napus L. var. oleífera; mustard; mass of a hundred grass; oilseeds.

scholarly journals Spontaneous gene flow from rapeseed ( Brassica napus ) to wild Brassica oleracea

2006 ◽  
Vol 273 (1605) ◽  
pp. 3111-3115 ◽  
Author(s):  
Caroline S Ford ◽  
Joël Allainguillaume ◽  
Phil Grilli-Chantler ◽  
Giulia Cuccato ◽  
Charlotte J Allender ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Gene Flow ◽  
Brassica Napus ◽  
Microsatellite Markers ◽  
Brassica Oleracea ◽  
Brassica Rapa ◽  
Environmental Risks ◽  
Wild Relatives ◽  
Similar Species ◽  
In The Wild

Research on the environmental risks of gene flow from genetically modified (GM) crops to wild relatives has traditionally emphasized recipients yielding most hybrids. For GM rapeseed ( Brassica napus ), interest has centred on the ‘frequently hybridizing’ Brassica rapa over relatives such as Brassica oleracea , where spontaneous hybrids are unreported in the wild. In two sites, where rapeseed and wild B. oleracea grow together, we used flow cytometry and crop-specific microsatellite markers to identify one triploid F 1 hybrid, together with nine diploid and two near triploid introgressants. Given the newly discovered capacity for spontaneous introgression into B. oleracea , we then surveyed associated flora and fauna to evaluate the capacity of both recipients to harm cohabitant species with acknowledged conservational importance. Only B. oleracea occupies rich communities containing species afforded legislative protection; these include one rare micromoth species that feeds on B. oleracea and warrants further assessment. We conclude that increased attention should now focus on B. oleracea and similar species that yield few crop-hybrids, but possess scope to affect rare or endangered associates.

Conservation of S-locus for self-incompatibility in Brassica napus (L.) and Brassica oleracea (L.)

1997 ◽  
Vol 95 (1-2) ◽  
pp. 73-82 ◽  
Author(s):  
W. Y. Cheung ◽  
G. Champagne ◽  
N. Hubert ◽  
L. Tulsieram ◽  
D. Charne ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Self Incompatibility ◽  
S Locus ◽  
Brassica Napus L ◽  
Brassica Oleracea L

Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa Cytoplasm

2010 ◽  
Vol 36 (8) ◽  
pp. 1280-1285 ◽  
Author(s):  
Jun LI ◽  
Li-Xia LUO ◽  
Zhuan WANG ◽  
Jun LI ◽  
Kun-Rong CHEN ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Brassica Rapa

scholarly journals A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L.

2011 ◽  
Vol 42 (No. 3) ◽  
pp. 103-110 ◽  
Author(s):  
N.D. Kaur ◽  
M. Vyvadilová ◽  
M. Klíma ◽  
M. Bechyně
Keyword(s):  
Brassica Napus ◽  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Growth Regulators ◽  
Protoplast Culture ◽  
Treatment Time ◽  
Simple Procedure ◽  
Enzyme Treatment ◽  
Induction Medium ◽  
Brassica Napus L

An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8&ndash;11.2 &times; 10<sup>4 </sup>protoplasts/ml in darkness at 25&deg;C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures were diluted with low osmotic medium on 7<sup>th</sup> and 11<sup>th</sup> day. The frequency of dividing cells was about 80% in B. oleracea and 50% in B. napus. After one month, the microcalli of approximately 0.5&ndash;1 mm in size were transferred into an induction medium with various combinations of growth regulators. Minimum duration of enzyme treatment time and extended dark period in the initial phase of culture increased the survival rate of protoplasts. Organogenesis started when the calli enlarged in size on an induction medium (1 mg/l NAA, 0.02 mg/l GA<sub>3</sub>, 1 mg/l 2iP) with 2% sucrose and 0.8% agar. Regeneration frequency of calli was found to be 69&ndash;75% in B. oleracea and 2&ndash;3% in B. napus. Well-developed shoots were transferred for rooting to a half-strength MS medium without growth regulators. More than 100 B. oleracea regenerants were transferred into soil, and they produced normal heads and set seeds. This very simple procedure is efficient and suitable mainly for B. oleracea var. botrytis and represents a background for fusion experiments. &nbsp;

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