Targeted resistance gene mapping in soybean using modified AFLPs

2000 ◽  
Vol 100 (8) ◽  
pp. 1279-1283 ◽  
Author(s):  
A. J. Hayes ◽  
M.A. Saghai Maroof

2011 ◽  
Vol 101 (5) ◽  
pp. 535-543 ◽  
Author(s):  
Mandy D. Kendrick ◽  
Donna K. Harris ◽  
Bo-Keun Ha ◽  
David L. Hyten ◽  
Perry B. Cregan ◽  
...  

Asian soybean rust (ASR) is an economically significant disease caused by the fungus Phakopsora pachyrhizi. The soybean genes Rpp3 and Rpp?(Hyuuga) confer resistance to specific isolates of the pathogen. Both genes map to chromosome 6 (Gm06) (linkage group [LG] C2). We recently identified 12 additional soybean accessions that harbor ASR resistance mapping to Gm06, within 5 centimorgans of Rpp3 and Rpp?(Hyuuga). To further characterize genotypes with resistance on Gm06, we used a set of eight P. pachyrhizi isolates collected from geographically diverse areas to inoculate plants and evaluate them for differential phenotypic responses. Three isolates elicited different responses from soybean accessions PI 462312 (Ankur) (Rpp3) and PI 506764 (Hyuuga) (Rpp?[Hyuuga]). In all, 11 of the new accessions yielded responses identical to either PI 462312 or Hyuuga and 1 of the new accessions, PI 417089B (Kuro daizu), differed from all others. Additional screening of Hyuuga-derived recombinant inbred lines indicated that Hyuuga carries two resistance genes, one at the Rpp3 locus on Gm06 and a second, unlinked ASR resistance gene mapping to Gm03 (LG-N) near Rpp5. These findings reveal a natural case of gene pyramiding for ASR resistance in Hyuuga and underscore the importance of utilizing multiple isolates of P. pachyrhizi when screening for ASR resistance.



2014 ◽  
Vol 40 (1) ◽  
pp. 54
Author(s):  
Bin LI ◽  
Yuan-Bao DENG ◽  
Xue-Hai YAN ◽  
Yang YANG ◽  
Peng-Qiang LIU ◽  
...  


PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0231157 ◽  
Author(s):  
Laura-Jayne Gardiner ◽  
Pauline Bansept-Basler ◽  
Mohamed El-Soda ◽  
Anthony Hall ◽  
Donal M. O’Sullivan


Author(s):  
W. Bernard

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.





Nature ◽  
2013 ◽  
Author(s):  
Ewen Callaway


2018 ◽  
Vol 51 (1) ◽  
Author(s):  
Xiaomei Li ◽  
Liu Liu ◽  
Xiangyu Liu ◽  
Yan Hou ◽  
Bingyin Xu ◽  
...  


Pathology ◽  
1984 ◽  
Vol 16 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Ronald J. Trent ◽  
P.E. Brock ◽  
J. Yakas ◽  
L.M. Trent ◽  
H. Kronenberg
Keyword(s):  


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