Characterization of Aegilops uniaristata chromosomes by comparative DNA marker analysis and repetitive DNA sequence in situ hybridization

2000 ◽  
Vol 101 (8) ◽  
pp. 1173-1179 ◽  
Author(s):  
N. Iqbal ◽  
S. M. Reader ◽  
P. D. S. Caligari ◽  
T. E. Miller
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Dna Marker ◽  
Marker Analysis ◽  
Repetitive Dna Sequence ◽  
Aegilops Uniaristata

Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1061-1069 ◽  
Author(s):  
A. Cuadrado ◽  
N. Jouve ◽  
C. Ceoloni
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
5S Rdna ◽  
Individual Identification ◽  
Highly Repetitive Dna ◽  
The Individual

The molecular characterization of heterochromatin in six lines of rye has been performed using fluorescence in situ hybridization (FISH). The highly repetitive rye DNA sequences pSc 119.2, pSc74, and pSc34, and the probes pTa71 and pSc794 containing the 25S–5.8S–18S rDNA (NOR) and the 5S rDNA multigene families, respectively, were used. This allowed the individual identification of all seven rye chromosomes and most chromosome arms in all lines. All varieties showed similar but not identical patterns. A standard in situ hybridization map was constructed following the nomenclature system recommended for C-bands. All FISH sites observed appeared to correspond well with C-band locations, but not all C-banding sites coincided with hybridization sites of the repetitive DNA probes used. Quantitative and qualitative differences between different varieties were found for in situ hybridization response at corresponding sites. Variation between plants and even between homologous chromosomes of the same plant was found in open-pollinated lines. In inbred lines, the in situ pattern of the homologues was practically identical and no variation between plants was detected. The observed quantitative and qualitative differences are consistent with a corresponding variation for C-bands detected both within and between cultivars.Key words: fluorescence in situ hybridization, repetitive DNA, rye, Secale cereale, polymorphism.

Cloning and Characterization of a Repetitive DNA Sequence Specific for Wuchereria bancrofti

1994 ◽  
Vol 51 (4) ◽  
pp. 495-500 ◽  
Author(s):  
Kithmini Siridewa ◽  
Ulf Pettersson ◽  
Lena Aslund ◽  
W. Abeyewickreme ◽  
Lena Franzen ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Wuchereria Bancrofti ◽  
Repetitive Dna Sequence

Cytological characterization of potato - Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 987-992 ◽  
Author(s):  
F Dong ◽  
R G Novy ◽  
J P Helgeson ◽  
J Jiang
Keyword(s):  
In Situ Hybridization ◽  
Dna Marker ◽  
Somatic Hybrids ◽  
Molecular Cytogenetics ◽  
Genomic In Situ Hybridization ◽  
Preferential Pairing ◽  
Potato Germplasm ◽  
Solanum Etuberosum

Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

Cloning and characterization of a rat-specific repetitive DNA sequence

Gene ◽  
1986 ◽  
Vol 45 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Scott K. Shore ◽  
Lee T. Bacheler ◽  
J. Kimball de Riel ◽  
Louis R. Barrows ◽  
Mark Lynch
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence

scholarly journals Characterization of a cloned repetitive DNA sequence concentrated on the human X chromosome.

1982 ◽  
Vol 79 (21) ◽  
pp. 6593-6597 ◽  
Author(s):  
T. P. Yang ◽  
S. K. Hansen ◽  
K. K. Oishi ◽  
O. A. Ryder ◽  
B. A. Hamkalo
Keyword(s):  
Dna Sequence ◽  
X Chromosome ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence

Cloning and characterisation of a new rye-specific repeated sequence

Genome ◽  
1991 ◽  
Vol 34 (1) ◽  
pp. 81-87 ◽  
Author(s):  
François Guidet ◽  
Peter Rogowsky ◽  
Christopher Taylor ◽  
Weining Song ◽  
Peter Langridge
Keyword(s):  
Molecular Marker ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Genetic Material ◽  
Repeated Sequence ◽  
Repeated Dna ◽  
New Family ◽  
Repetitive Dna Sequence ◽  
Repeated Dna Families

In this paper we describe a repetitive DNA sequence unique to the rye genome. The corresponding DNA probe (pAW173) was cloned as a 450-bp fragment and does not hybridize to wheat or barley DNA and is not related to five other repeated DNA families characterised in rye. This new family (designated the R173 family) consists of moderately repeated DNA (~ 15 000 units per genome) and is present on all seven rye chromosomes. The hybridization patterns in Southern blots indicate that it is not organised as blocks of tandem arrays and in situ hybridization shows that it is dispersed throughout the rye chromosomes. The probe has been successfully used as a molecular marker in identifying rye genetic material in a wheat background.Key words: repetitive DNA sequence, rye-specific DNA, molecular marker.

Cloning and characterization of a species-specific repetitive DNA sequence from Onchocerca armillata

1988 ◽  
Vol 30 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Katherine A. Murray ◽  
Rory J. Post ◽  
Julian M. Crampton ◽  
Philip J. McCall ◽  
Boubacar Kouyate
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence ◽  
Species Specific
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