Isolation and Characterization of a Repetitive DNA Sequence from Leishmania infantum: Development of a Visceral Leishmaniasis Polymerase Chain Reaction

1993 ◽  
Vol 49 (3) ◽  
pp. 364-369 ◽  
Author(s):  
R. Piarroux ◽  
H. Dumon ◽  
M. Quilici ◽  
R. Azaiez ◽  
A. M. Lossi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Leishmania Infantum ◽  
Chain Reaction ◽  
Isolation And Characterization ◽  
Repetitive Dna Sequence ◽  
Polymerase Chain

scholarly journals Isolation and Characterization of Micro-organisms with Industrial Importance From Sisal Bole Rots

2013 ◽  
Vol 34 (1) ◽  
pp. 26-35
Author(s):  
Consolatha J.N. Mhaiki ◽  
Enock Masanja ◽  
Jamidu H.Y. Katima ◽  
Gunaraths Rajarao ◽  
Gunnel Dalhammar
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Isolation And Characterization ◽  
Brevundimonas Diminuta ◽  
Industrial Use ◽  
Brevundimonas Vesicularis ◽  
Polymerase Chain ◽  
The Individual ◽  
Micro Organisms

Investigation of microorganisms naturally acclimatized to Agave hybrid H 11648 (sisal bole rot) was conducted, with the aim of isolating and characterizing Aspergillus niger strains for industrial use. Microorganism were identified morphologically and then confirmation made by polymerase chain reaction (PCR). Results showed the existence of four major groups, listed in order of abundances as follows; Aspergilli (36.0±0.8) %, Penicillin (28.0±0.1) %, Yeast (15.0±1.6) %and Fusarium (10.0±0.12) %. The main groups of Aspergilli strains were A. nidulans, A. tamari and A. niger in ratios (3:2:2), respectively. Several endo-spore forming non-enteric gram (-) rods and coccid bacteria identified by API20 NE identification systemincluded,Brevundimonas diminuta sp, Shewanella putrefaciens sp, Brevundimonas vesicularis sp and Pasteurella sp. Results showed that sisal bole rot stems hosts a high bio-diversity of microorganism species other than A. niger. Exploitation of the individual strains is recommended. This could eventually produce strains forprecursors of industrially and therapeutically metabolites.

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