Time-Dependent Responses of Oxidative Stress, Growth, and Reproduction of Daphnia magna Under Thermal Stress

Abstract Blast lung injury (BLI) is the major cause of death in explosion-derived shock waves; however, the mechanisms of BLI are not well understood. To identify the time-dependent manner of BLI, a model of lung injury of rats induced by shock waves was established by a fuel air explosive. The model was evaluated by hematoxylin and eosin staining and pathological score. The inflammation and oxidative stress of lung injury were also investigated. The pathological scores of rats’ lung injury at 2 h, 24 h, 3 days, and 7 days post-blast were 9.75±2.96, 13.00±1.85, 8.50±1.51, and 4.00±1.41, respectively, which were significantly increased compared with those in the control group (1.13±0.64; P<0.05). The respiratory frequency and pause were increased significantly, while minute expiratory volume, inspiratory time, and inspiratory peak flow rate were decreased in a time-dependent manner at 2 and 24 h post-blast compared with those in the control group. In addition, the expressions of inflammatory factors such as interleukin (IL)-6, IL-8, FosB, and NF-κB were increased significantly at 2 h and peaked at 24 h, which gradually decreased after 3 days and returned to normal in 2 weeks. The levels of total antioxidant capacity, total superoxide dismutase, and glutathione peroxidase were significantly decreased 24 h after the shock wave blast. Conversely, the malondialdehyde level reached the peak at 24 h. These results indicated that inflammatory and oxidative stress induced by shock waves changed significantly in a time-dependent manner, which may be the important factors and novel therapeutic targets for the treatment of BLI.

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Human Proximal Tubule Epithelial Cells (HK-2) as a Sensitive In Vitro System for Ochratoxin A Induced Oxidative Stress

Toxins ◽

10.3390/toxins13110787 ◽

2021 ◽

Vol 13 (11) ◽

pp. 787

Author(s):

Enrique García-Pérez ◽

Dojin Ryu ◽

Hwa-Young Kim ◽

Hae Dun Kim ◽

Hyun Jung Lee

Keyword(s):

Oxidative Stress ◽

Ochratoxin A ◽

Cell Lines ◽

Time Dependent ◽

Mrna Levels ◽

Dependent Manner ◽

In Vitro System ◽

Glutathione Peroxidase 1 ◽

Glucose 6 Phosphate Dehydrogenase

Ochratoxin A (OTA) is a mycotoxin that is potentially carcinogenic to humans. Although its mechanism remains unclear, oxidative stress has been recognized as a plausible cause for the potent renal carcinogenicity observed in experimental animals. The effect of OTA on oxidative stress parameters in two cell lines of LLC-PK1 and HK-2 derived from the kidneys of pig and human, respectively, were investigated and compared. We found that the cytotoxicity of OTA on LLC-PK1 and HK-2 cells was dose- and time-dependent in both cell lines. Furthermore, increased intracellular reactive oxygen species (ROS) induced by OTA in both cell lines were observed in a time-dependent manner. Glutathione (GSH) was depleted by OTA at >48 h in HK-2 but not in LLC-PK1 cells. While the mRNA levels of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase 1 (GPX1) in LLC-PK1 were down-regulated by 0.67- and 0.66-fold, respectively, those of catalase (CAT), glutathione reductase (GSR), and superoxide dismutase 1 (SOD) in HK-2 were up-regulated by 2.20-, 2.24-, and 2.75-fold, respectively, after 72 h exposure to OTA. Based on these results, we conclude that HK-2 cells are more sensitive to OTA-mediated toxicity than LLC-PK1, and OTA can cause a significant oxidative stress in HK-2 as indicated by changes in the parameter evaluated.

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Renal effects of deltamethrin induced intoxication in Carassius auratus gibelio (Pisces cyprinidae)

Biotechnology in Animal Husbandry ◽

10.2298/bah0702399c ◽

2007 ◽

Vol 23 (5-6-2) ◽

pp. 399-404 ◽

Cited By ~ 2

Author(s):

D. Costin ◽

A. Staicu ◽

R. Huculeci ◽

G. Stoian ◽

M. Costache ◽

...

Keyword(s):

Oxidative Stress ◽

Glutathione Reductase ◽

Carassius Auratus ◽

Pesticide Exposure ◽

Time Dependent ◽

Dependent Manner ◽

Carassius Auratus Gibelio ◽

Renal Effects ◽

Goldfish Carassius Auratus ◽

Antioxidant Mechanisms

Freshwater goldfish Carassius auratus gibelio were exposed to 2?g/l delthametrin for one, 2, 3, 7 and 14 days. Activities of kidney catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2) and glutathione-Stransferase (EC 2.5.1.18) were affected in a time-dependent manner by the pesticide exposure compared to controls. The results indicate that C. auratus gibelio kidney resisted to oxidative stress by antioxidant mechanisms and developed an adaptative response.

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Assessment of ecotoxicological effects of ciprofloxacin in Daphnia magna: life-history traits, biochemical and genotoxic effects

Water Science & Technology ◽

10.2166/wst.2018.255 ◽

2018 ◽

Vol 2017 (3) ◽

pp. 835-844 ◽

Cited By ~ 1

Author(s):

B. Nunes ◽

C. Leal ◽

S. Rodrigues ◽

S. C. Antunes

Keyword(s):

Oxidative Stress ◽

Life History ◽

Daphnia Magna ◽

Metabolic Response ◽

Damage Index ◽

Continuous Exposure ◽

Genetic Damage ◽

Genotoxic Effects ◽

Damage Scenarios ◽

Life History Parameters

Abstract Antibiotics (e.g. ciprofloxacin) have been detected in surface water and groundwater for several decades. In order to understand the potential impact of the continuous exposure of aquatic organisms to ciprofloxacin, a chronic assay was carried out with Daphnia magna. This approach allowed evaluation of the effects of ciprofloxacin on life-history and sub-individual parameters (antioxidant status and metabolic response: activities of catalase and glutathione S-transferases – GSTs; peroxidative damage; thiobarbituric acid reactive substances and genotoxic effects (genetic damage index, measured by the comet assay). Life-history parameters of D. magna showed no significant effects after ciprofloxacin exposure. Concerning oxidative stress and metabolism parameters, no significant alterations were reported for catalase and GSTs activities. However, a dual response was observed, with a significant decrease in lipid peroxidation levels at low ciprofloxacin concentrations (<0.013 mg/L), while a significant increase was verified at high ciprofloxacin concentrations (0.078 mg/L). The genotoxicity assay detected a significant increase in genetic damage index up to 0.013 mg/L of ciprofloxacin. The here-tested ciprofloxacin concentrations, which are ecologically relevant, did not cause significant impacts concerning the life-history parameters of D. magna; however, at the same levels of ciprofloxacin an oxidative stress and genotoxic damage scenarios were recorded.

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Psychoactive drugs citalopram and mirtazapine caused oxidative stress and damage of feeding behavior in Daphnia magna

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2021.113147 ◽

2022 ◽

Vol 230 ◽

pp. 113147

Author(s):

Shengzi Duan ◽

Yourong Fu ◽

Shanshan Dong ◽

Yunfeng Ma ◽

Hangyu Meng ◽

...

Keyword(s):

Oxidative Stress ◽

Feeding Behavior ◽

Daphnia Magna ◽

Psychoactive Drugs

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Effect of algal ration - quality and method of quantification - on growth and reproduction of Daphnia magna

Archiv für Hydrobiologie ◽

10.1127/archiv-hydrobiol/125/1992/311 ◽

1992 ◽

Vol 125 (3) ◽

pp. 311-321

Author(s):

Caroline Naylor ◽

Mairead C. Bradley ◽

Peterc Calow

Keyword(s):

Daphnia Magna ◽

Growth And Reproduction

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Tamarix articulata Extracts Exhibit Antioxidant Activity and Offer Protection against Hydrogen Peroxide-Mediated Toxicity to Human Skin Fibroblasts

Journal of Toxicology ◽

10.1155/2020/8896263 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Abdullah M. Alnuqaydan

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Protective Effect ◽

Mtt Assay ◽

Skin Fibroblasts ◽

Time Dependent ◽

Significant Protection ◽

Methanolic Extracts ◽

Higher Doses ◽

Oxidative Insult

Tamarix articulata (TA) is a wild halophytic plant growing in extremely harsh environmental conditions in the deserts of Saudi Arabia. Evaluating the protective effect of the methanolic extract of different parts (fresh and dry leaves, stem, and root) of TA was determined by MTT assay using Hs27 skin fibroblasts as the cellular model. The study was designed and conducted in two sets. The first set assesses the toxicity profile of TA extracts in both concentration- and time-dependent ways on Hs27 cells. Our MTT results showed that methanolic extracts from all four parts of TA at varying doses (27.5, 55, 110, and 220 μg/mL) display negligible toxicity when exposed for 4 h. However, exposure of Hs27 cells to varying doses of all four TA extracts for 24 and 48 h promotes significant 23%, 24%, 26%, and 25% p < 0.05 and 35%, 36%, 39%, and 41% p < 0.05 cell toxicity at 220 μg/mL of all four TA extracts compared to untreated control cells. To evaluate the protection offered by TA extracts against H₂O₂, we perform a second set of experiments to preincubate Hs27 cells with the TA extracts in both dose- and time-dependent way. This is followed by 300 μM hydrogen peroxide- (H₂O₂-) mediated oxidative insult for 1 h. Using MTT assay, we found that methanolic extracts of TA at different time points (4, 24, and 48 h) and higher doses (220 μg/mL) provide significant protection in cell viability when challenged with H2O2-induced oxidative stress in Hs27 cells. The protective effect was more pronounced at 48 h and 220 μg/mL and the amounts were 39%, 41%, 41%, and 44% for stem, root, fresh leaf, and dry leaf TA extracts p < 0.05 , respectively, compared to untreated cells (2–4%). Collectively, the current study demonstrates that methanolic extracts of TA contain potential bioactive compounds and offer significant protection against H2O2-mediated oxidative stress in Hs27 skin fibroblasts.

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