Benzene-induced mutational pattern in the tumour suppressor gene TP53 analysed by use of a functional assay, the functional analysis of separated alleles in yeast, in human lung cells

2009 ◽  
Vol 84 (2) ◽  
pp. 99-107 ◽  
Author(s):  
Sylvain Billet ◽  
Vincent Paget ◽  
Guillaume Garçon ◽  
Natacha Heutte ◽  
Véronique André ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Human Lung ◽  
Suppressor Gene ◽  
Tumour Suppressor Gene ◽  
Tumour Suppressor ◽  
Lung Cells ◽  
Functional Assay ◽  
Human Lung Cells ◽  
Mutational Pattern ◽  
Gene Tp53

scholarly journals Characterising Mutational Spectra of Carcinogens in the Tumour Suppressor Gene TP53 Using Human TP53 Knock-in (Hupki) Mouse Embryo Fibroblasts

2019 ◽  
Vol 2 (4) ◽  
pp. 85 ◽  
Author(s):  
Hölzl-Armstrong ◽  
Kucab ◽  
Korenjak ◽  
Luijten ◽  
Phillips ◽  
...  
Keyword(s):  
Tp53 Mutation ◽  
Human Cancer ◽  
Atmospheric Oxygen ◽  
Suppressor Gene ◽  
Tumour Suppressor Gene ◽  
Tumour Suppressor ◽  
International Agency ◽  
Human Tumours ◽  
Clonal Cell Lines ◽  
Gene Tp53

DNA in dividing cells is prone to mutagenesis, with mutations making key contributions to human disease including cancer. The tumour suppressor gene TP53 is the most frequently mutated gene in human tumours. Here, we present a robust protocol for studying TP53 mutagenesis utilising human TP53 knock-in (Hupki) mouse embryonic fibroblasts (HUFs). In the HUF immortalisation assay (HIMA), primary HUFs are treated with known or suspected carcinogens at 3% oxygen and then transferred to 20% atmospheric oxygen to induce senescence. Cells containing mutations (e.g., in TP53) that allow bypassing of senescence eventually emerge as immortalised clonal cell lines after 2–3 months of serial passaging. As not all immortalised HUF cells contain TP53 mutations, we developed a Nutlin-3a counter-screen to select for TP53-mutated clones prior to sequencing. TP53 mutation spectra generated can be compared with those of human tumours recorded in the International Agency for Research on Cancer TP53 mutation database. Environmental mutagens that have demonstrated and validated the utility of the HIMA include ultraviolet radiation, aristolochic acid, and benzo[a]pyrene. The TP53 mutation patterns induced by these mutagens in the HIMA corresponded to those found in human tumours from patients exposed to these mutagens. The approach presented helps to deepen our understanding of human cancer aetiology.

scholarly journals Tumour suppressor gene TP53 mutations in atypical vascular lesions of breast skin following radiotherapy

2011 ◽  
Vol 58 (3) ◽  
pp. 455-466 ◽  
Author(s):  
Raffaella Santi ◽  
Valentina Cetica ◽  
Alessandro Franchi ◽  
Monica Pepi ◽  
Anna M Cesinaro ◽  
...  
Keyword(s):  
Suppressor Gene ◽  
Tumour Suppressor Gene ◽  
Tumour Suppressor ◽  
Vascular Lesions ◽  
Tp53 Mutations ◽  
Gene Tp53

tumour suppressor gene and the tobacco industry

The Lancet ◽  
2005 ◽  
Vol 365 (9464) ◽  
pp. 1026-1027
Author(s):  
A BITTON ◽  
M NEUMAN ◽  
J BARNOYA ◽  
S GLANTZ
Keyword(s):  
Tobacco Industry ◽  
Suppressor Gene ◽  
Tumour Suppressor Gene ◽  
Tumour Suppressor
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