Cytotoxicity of novel fluorinated alternatives to long-chain perfluoroalkyl substances to human liver cell line and their binding capacity to human liver fatty acid binding protein

2017 ◽  
Vol 92 (1) ◽  
pp. 359-369 ◽  
Author(s):  
Nan Sheng ◽  
Ruina Cui ◽  
Jinghua Wang ◽  
Yong Guo ◽  
Jianshe Wang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Liver ◽  
Fatty Acid Binding Protein ◽  
Binding Capacity ◽  
Perfluoroalkyl Substances ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Human Liver Cell ◽  
Long Chain

scholarly journals Up-regulation of the expression of the gene for liver fatty acid-binding protein by long-chain fatty acids

1996 ◽  
Vol 319 (2) ◽  
pp. 483-487 ◽  
Author(s):  
Claire MEUNIER-DURMORT ◽  
Hélène POIRIER ◽  
Isabelle NIOT ◽  
Claude FOREST ◽  
Philippe BESNARD
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Fold Increase ◽  
Long Chain Fatty Acids ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Fabp Gene ◽  
Long Chain

The role of fatty acids in the expression of the gene for liver fatty acid-binding protein (L-FABP) was investigated in the well-differentiated FAO rat hepatoma cell line. Cells were maintained in serum-free medium containing 40 µM BSA/320 µM oleate. Western blot analysis showed that oleate triggered an approx. 4-fold increase in the cytosolic L-FABP level in 16 h. Oleate specifically stimulated L-FABP mRNA in time-dependent and dose-dependent manners with a maximum 7-fold increase at 16 h in FAO cells. Preincubation of FAO cells with cycloheximide prevented the oleate-mediated induction of L-FABP mRNA, showing that protein synthesis was required for the action of fatty acids. Run-on transcription assays demonstrated that the control of L-FABP gene expression by oleate was, at least in part, transcriptional. Palmitic acid, oleic acid, linoleic acid, linolenic acid and arachidonic acid were similarly potent whereas octanoic acid was inefficient. This regulation was also found in normal hepatocytes. Therefore long-chain fatty acids are strong inducers of L-FABP gene expression. FAO cells constitute a useful tool for studying the underlying mechanism of fatty acid action.

scholarly journals Bile salt recognition by human liver fatty acid binding protein

FEBS Journal ◽  
2015 ◽  
Vol 282 (7) ◽  
pp. 1271-1288 ◽  
Author(s):  
Filippo Favretto ◽  
Carlo Santambrogio ◽  
Mariapina D'Onofrio ◽  
Henriette Molinari ◽  
Rita Grandori ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Bile Salt ◽  
Human Liver ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Structural and functional interaction of fatty acids with human liver fatty acid-binding protein (L-FABP) T94A variant

FEBS Journal ◽  
2014 ◽  
Vol 281 (9) ◽  
pp. 2266-2283 ◽  
Author(s):  
Huan Huang ◽  
Avery L. McIntosh ◽  
Gregory G. Martin ◽  
Kerstin K. Landrock ◽  
Danilo Landrock ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Human Liver ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Functional Interaction ◽  
Protein L ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Molecular differences between human liver fatty acid binding protein and its T94A variant in their unbound and lipid-bound states

2017 ◽  
Vol 1865 (9) ◽  
pp. 1152-1159 ◽  
Author(s):  
Mariapina D'Onofrio ◽  
Carlo Giorgio Barracchia ◽  
Andrea Bortot ◽  
Francesca Munari ◽  
Serena Zanzoni ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Liver ◽  
Bound States ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein
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