Gender comparison of muscarinic receptor expression and function in rat and human urinary bladder: differential regulation of M2 and M3 receptors?

From mammals to fish, reproduction is driven by luteinizing hormone (LH) and follicle-stimulating hormone (FSH) temporally secreted from the pituitary gland. Teleost fish are an excellent model for addressing the unique regulation and function of each gonadotropin cell since, unlike mammals, they synthesize and secrete LH and FSH from distinct cells. Only very distant vertebrate classes (such as fish and birds) demonstrate the mono-hormonal strategy, suggesting a potential convergent evolution. Cell-specific transcriptome analysis of double-labeled transgenic tilapia expressing GFP and RFP in LH or FSH cells, respectively, yielded genes specifically enriched in each cell type, revealing differences in hormone regulation, receptor expression, cell signaling, and electrical properties. Each cell type expresses a unique GPCR signature that reveals the direct regulation of metabolic and homeostatic hormones. Comparing these novel transcriptomes to that of rat gonadotrophs revealed conserved genes that might specifically contribute to each gonadotropin activity in mammals, suggesting conserved mechanisms controlling the differential regulation of gonadotropins in vertebrates.

Download Full-text

Regulation of M3 Muscarinic Receptor Expression and Function by Transmembrane Protein 147

Molecular Pharmacology ◽

10.1124/mol.110.067363 ◽

2010 ◽

Vol 79 (2) ◽

pp. 251-261 ◽

Cited By ~ 16

Author(s):

Erica Rosemond ◽

Mario Rossi ◽

Sara M. McMillin ◽

Marco Scarselli ◽

Julie G. Donaldson ◽

...

Keyword(s):

Muscarinic Receptor ◽

Transmembrane Protein ◽

Receptor Expression ◽

And Function ◽

M3 Muscarinic Receptor

Download Full-text

Trigone of human urinary bladder is a novel site of luteinizing hormone / chorionic gonadotropin receptor expression.

Journal of the Society for Gynecologic Investigation ◽

10.1016/1071-5576(96)83061-9 ◽

1996 ◽

Vol 3 (2) ◽

pp. 365A

Author(s):

X Y

Keyword(s):

Luteinizing Hormone ◽

Urinary Bladder ◽

Chorionic Gonadotropin ◽

Receptor Expression ◽

Human Urinary Bladder ◽

Gonadotropin Receptor

Download Full-text

Regulation of muscarinic receptor expression and function in cultured cells and in knock-out mice

Life Sciences ◽

10.1016/s0024-3205(97)00053-2 ◽

1997 ◽

Vol 60 (13-14) ◽

pp. 1101-1104 ◽

Cited By ~ 6

Author(s):

Lise A. McKinnon ◽

Marc Rosoff ◽

Susan E. Hamilton ◽

Michael L. Schlador ◽

Sarabeth L. Thomas ◽

...

Keyword(s):

Muscarinic Receptor ◽

Cultured Cells ◽

Receptor Expression ◽

Knock Out ◽

Knock Out Mice ◽

And Function

Download Full-text

Effects of Pilocarpine, a Muscarinic Receptor Agonist, on Contraction of the Pig and Human Urinary Bladder

Clinics in Surgery ◽

10.25107/cis-v5-id2898 ◽

2016 ◽

Vol 5 (1) ◽

pp. 5-1

Author(s):

Tomonori Yamanishi ◽

Tomohiko Kamasako ◽

Kanya Kaga ◽

Mayuko Kaga ◽

Miki Fuse ◽

...

Keyword(s):

Urinary Bladder ◽

Muscarinic Receptor ◽

Receptor Agonist ◽

Human Urinary Bladder

Download Full-text

Molecular analysis of the regulation of muscarinic receptor expression and function

Life Sciences ◽

10.1016/0024-3205(95)00031-z ◽

1995 ◽

Vol 56 (11-12) ◽

pp. 939-943 ◽

Cited By ~ 3

Author(s):

Susan E. Hamilton ◽

Lise A. McKinnon ◽

Darrell A. Jackson ◽

Phyllis S. Goldman ◽

Jacques C. Migeon ◽

...

Keyword(s):

Muscarinic Receptor ◽

Molecular Analysis ◽

Receptor Expression ◽

And Function

Download Full-text

Distribution and function of muscarinic receptor subtypes in the ovine submandibular gland

Journal of Applied Physiology ◽

10.1152/japplphysiol.00779.2005 ◽

2006 ◽

Vol 100 (4) ◽

pp. 1215-1223 ◽

Cited By ~ 19

Author(s):

G. Tobin ◽

A. T. Ryberg ◽

S. Gentle ◽

A. V. Edwards

Keyword(s):

Submandibular Gland ◽

Muscarinic Receptor ◽

Secretory Response ◽

Receptor Subtypes ◽

Muscarinic Receptor Antagonists ◽

M3 Receptors ◽

M1 Receptors ◽

Tissue Specimens ◽

And Function ◽

Protein Output

The effects of muscarinic receptor antagonists on responses to electrical stimulation of the chorda-lingual nerve were determined in pentobarbitone-anesthetized sheep and correlated to the morphology of tissue specimens. Stimulation at 2 Hz continuously, or in bursts of 1 s at 20 Hz every 10 s, for 10 min induced similar submandibular fluid responses (19 ± 3 vs. 21 ± 3 μl·min−1·g gland−1), whereas vasodilatation was greater during stimulation in bursts (−52 ± 4 vs. −43 ± 5%; P < 0.01). Continuous stimulation at 8 Hz induced substantially greater responses (66 ± 9 μl·min−1·g gland−1 and −77 ± 3%). While atropine (0.5 mg/kg iv) abolished the secretory response at 2 and 20 Hz (1:10 s), a small response persisted at 8 Hz (<5%). The “M1-selective” antagonist pirenzepine (40 μg/kg iv) reduced the fluid response at all frequencies tested ( P < 0.05–0.01), most conspicuously at 2 Hz (reduced by 69%). Methoctramine (“M2/M4-selective”; 100 μg/kg iv; n = 5) had no effect on fluid or the vascular responses but increased the protein output at 2 (+90%, P < 0.05) and 8 Hz (+45%, P < 0.05). The immunoblotting showed distinct bands for muscarinic M1, M3, M4, and M5 receptors, and immunohistochemistry showed muscarinic M1 and M3 receptors to occur in the parenchyma. Thus muscarinic M1 receptors contribute to the secretory response to parasympathetic stimulation but have little effect on the vasodilatation in the ovine submandibular gland. Increased transmitter release caused by blockade of neuronal inhibitory receptors of the M4 subtype would explain the increase in protein output.

Download Full-text