DNA microarrays for visual detection of human pathogenic microorganisms based on tyramine signal amplification coupled with gold label silver stain

2010 ◽  
Vol 398 (6) ◽  
pp. 2745-2750 ◽  
Author(s):  
Hong-Juan Qi ◽  
Su-Hong Chen ◽  
Min-Li Zhang ◽  
Hua Shi ◽  
Sheng-Qi Wang
Keyword(s):  
Signal Amplification ◽  
Dna Microarrays ◽  
Visual Detection ◽  
Pathogenic Microorganisms ◽  
Silver Stain ◽  
Gold Label

The detection of a transgenic soybean biochip using gold label silver stain technology

2011 ◽  
Vol 21 (22) ◽  
pp. 6905-6908 ◽  
Author(s):  
Zhongliang Deng ◽  
Yuetao Ge ◽  
Qike Cao ◽  
Ke Han
Keyword(s):  
Transgenic Soybean ◽  
Silver Stain ◽  
Gold Label

A molecular Fe-complex as a catalyst probe for in-gel visual detection of proteins via signal amplification

2015 ◽  
Vol 51 (83) ◽  
pp. 15257-15260 ◽  
Author(s):  
Sushma Kumari ◽  
Chakadola Panda ◽  
Shyamalava Mazumdar ◽  
Sayam Sen Gupta
Keyword(s):  
Signal Amplification ◽  
Visual Detection ◽  
Polyacrylamide Gel ◽  
Peroxidase Mimic ◽  
Fe Complex

We report the use of a molecular peroxidase mimic biuret FeTAML for chemoselective labeling of proteins and the subsequent visual detection (<0.1 pmoles) of the conjugate in a polyacrylamide gel by catalytic signal amplification.

Comparison of detection and signal amplification methods for DNA microarrays

2008 ◽  
Vol 22 (5-6) ◽  
pp. 294-300 ◽  
Author(s):  
Gary J. Vora ◽  
Carolyn E. Meador ◽  
George P. Anderson ◽  
Chris Rowe Taitt
Keyword(s):  
Signal Amplification ◽  
Dna Microarrays

Rapid and visual detection of Listeria monocytogenes based on nanoparticle cluster catalyzed signal amplification

2016 ◽  
Vol 86 ◽  
pp. 1-7 ◽  
Author(s):  
Lisha Zhang ◽  
Ru Huang ◽  
Weipeng Liu ◽  
Hongxing Liu ◽  
Xiaoming Zhou ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Signal Amplification ◽  
Visual Detection ◽  
Nanoparticle Cluster

A novel electrochemical DNA detection method based on bio-barcode/gold label silver stain dual amplification

2020 ◽  
Vol 10 (2) ◽  
pp. 206-213
Author(s):  
Pengfei Jiang ◽  
Miduo Tan ◽  
Lan Zhao ◽  
Yulin Wang ◽  
Ting Pan ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Detection Method ◽  
Linear Sweep Voltammetry ◽  
Silica Nanoparticle ◽  
Silver Ions ◽  
Silver Deposition ◽  
Silver Stain ◽  
Modified Dna ◽  
Gold Label ◽  
Dual Amplification

A novel electrochemical gene detection method based on bio-barcode/gold label silver stain (GLSS) dual amplification is presented. The silica nanoparticle/gold nanoparticles (SiO2/AuNPs) barcode probes were constructed by combining SiO2 nanoparticle-labeled DNA with AuNPs, which hybridizes with target DNAs and the electrode surface-modified DNA probes. The silver ions were reduced on the surface of AuNPs to form a silver shell by the catalysis of AuNPs, and then the electrochemical detection of deposited silver was performed by linear sweep voltammetry (LSV). In this paper, the effects of electrode blocking agent, blocking time and silver deposition time on electrochemical detection were also investigated. The proposed electrochemical DNA biosensor bring forth a low detection limit of 0.23 fM and a widely linear range of 1 fM to 10 pM. This method can clearly distinguish the complementary and mismatched DNA, with good repeatability and stability.

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