The detection of a transgenic soybean biochip using gold label silver stain technology

2011 ◽  
Vol 21 (22) ◽  
pp. 6905-6908 ◽  
Author(s):  
Zhongliang Deng ◽  
Yuetao Ge ◽  
Qike Cao ◽  
Ke Han
Keyword(s):  
Transgenic Soybean ◽  
Silver Stain ◽  
Gold Label

A novel electrochemical DNA detection method based on bio-barcode/gold label silver stain dual amplification

2020 ◽  
Vol 10 (2) ◽  
pp. 206-213
Author(s):  
Pengfei Jiang ◽  
Miduo Tan ◽  
Lan Zhao ◽  
Yulin Wang ◽  
Ting Pan ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Detection Method ◽  
Linear Sweep Voltammetry ◽  
Silica Nanoparticle ◽  
Silver Ions ◽  
Silver Deposition ◽  
Silver Stain ◽  
Modified Dna ◽  
Gold Label ◽  
Dual Amplification

A novel electrochemical gene detection method based on bio-barcode/gold label silver stain (GLSS) dual amplification is presented. The silica nanoparticle/gold nanoparticles (SiO2/AuNPs) barcode probes were constructed by combining SiO2 nanoparticle-labeled DNA with AuNPs, which hybridizes with target DNAs and the electrode surface-modified DNA probes. The silver ions were reduced on the surface of AuNPs to form a silver shell by the catalysis of AuNPs, and then the electrochemical detection of deposited silver was performed by linear sweep voltammetry (LSV). In this paper, the effects of electrode blocking agent, blocking time and silver deposition time on electrochemical detection were also investigated. The proposed electrochemical DNA biosensor bring forth a low detection limit of 0.23 fM and a widely linear range of 1 fM to 10 pM. This method can clearly distinguish the complementary and mismatched DNA, with good repeatability and stability.

In situSynthesis of Oligonucleotide and Detection of Gold-label-silver-stain on PTFE Slices

2005 ◽  
Vol 23 (9) ◽  
pp. 1247-1252
Author(s):  
Mei-Jun Tan ◽  
Jian-Xin Tang ◽  
Hong Chen
Keyword(s):  
Silver Stain ◽  
Gold Label

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

Antisense RNA-mediated GmFAD2-1B Gene Silencing Enhances Accumulation of Oleic Acid in Transgenic Soybean Seeds

2017 ◽  
Vol 43 (11) ◽  
pp. 1588 ◽  
Author(s):  
Jing YANG ◽  
Guo-Jie XING ◽  
Lu NIU ◽  
Hong-Li HE ◽  
Qian DU ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Gene Silencing ◽  
Antisense Rna ◽  
Soybean Seeds ◽  
Transgenic Soybean

Production of γ-Linolenic Acid and Stearidonic Acid in Seeds of Marker-Free Transgenic Soybean

Crop Science ◽  
2004 ◽  
Vol 44 (2) ◽  
pp. 646 ◽  
Author(s):  
Shirley Sato ◽  
Aiqiu Xing ◽  
Xingguo Ye ◽  
Bruce Schweiger ◽  
Anthony Kinney ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Stearidonic Acid ◽  
Transgenic Soybean ◽  
Marker Free
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