Pyoverdine, a fluorescent siderophore that have high- affinity for iron is produced by Pseudomonas putida and Pseudomonas aeruginosa that synthesizes it under iron-deficient growth conditions. Pseudomonas species are often encountered in diverse ecological habitats along side being censurable for nosocomial infections spread round the world. Due to these characteristics, there's a growing interest during this microbe for a spread of uses. One such is the production of Pyoverdine, it influences the uptake of iron, along with eliminating the limited iron availability condition.
Pyoverdine is important for Pseudomonas putida and Pseudomonas aeruginosa to cause acute infections. Intense research and study led to the invention of Pyoverdines being a crucial source of chelating Iron. This study is predicated on Pyoverdine extracted from two different species of Pseudomonas which will act as an antimicrobial agent for various species including Escherichia coli and Staphylococcus aureus. The organism, i.e., Pseudomonas putida and Pseudomonas aeruginosa, were isolated from soil sample using medias like: Cetrimide media, King’s B media. It had been confirmed using primary biochemical tests, along side species level identification (MALDI - TOF). Isolation was followed by studying the antimicrobial activity of Pyoverdine on different organisms using antibiotics as standard for the same. Results for the tests were obtained, colonies were observed on specific media and zone of inhibition was observed on Muller Hinton plate. Comparative studies were carried out to find which organism used up Pyoverdine or Pyoverdine - Fe complex without using FeCl3 as sole standard source. Thus, these compounds can synergize with conventional antimicrobials, forming a simpler treatment with serving as a useful gizmo