The Advantages of Bilateral Osteotomy Over Unilateral Osteotomy for Osteoporotic Bone Healing

2018 ◽  
Vol 103 (1) ◽  
pp. 80-94 ◽  
Author(s):  
M. Komrakova ◽  
J. Fiebig ◽  
D. B. Hoffmann ◽  
C. Krischek ◽  
W. Lehmann ◽  
...  
Keyword(s):  
Bone Healing ◽  
Osteoporotic Bone

scholarly journals A metaphyseal fracture rat model for mechanistic studies of osteoporotic bone healing

2019 ◽  
Vol 37 ◽  
pp. 420-430 ◽  
Author(s):  
RMY Wong ◽  
U Thormann ◽  
MHV Choy ◽  
NY Chim ◽  
MCM Li ◽  
...  
Keyword(s):  
Bone Healing ◽  
Rat Model ◽  
Osteoporotic Bone ◽  
Mechanistic Studies ◽  
Metaphyseal Fracture

scholarly journals Potential application of nano-hydroxyapatite in treatment of osteoporotic bone healing - a preliminary study

2016 ◽  
Vol 4 ◽  
Author(s):  
Yang Xiao ◽  
Xie Pengfei ◽  
Chen Xuening ◽  
Zhu Xiangdong ◽  
Fan Yujiang ◽  
...  
Keyword(s):  
Bone Healing ◽  
Potential Application ◽  
Osteoporotic Bone ◽  
Preliminary Study

Controlled-release naringin nanoscaffold for osteoporotic bone healing

2014 ◽  
Vol 30 (11) ◽  
pp. 1263-1273 ◽  
Author(s):  
Yan Ji ◽  
Lu Wang ◽  
David C. Watts ◽  
Hongmei Qiu ◽  
Tao You ◽  
...  
Keyword(s):  
Controlled Release ◽  
Bone Healing ◽  
Osteoporotic Bone

Effect of zileuton on osteoporotic bone and its healing, expression of bone, and brain genes in rats

2018 ◽  
Vol 124 (1) ◽  
pp. 118-130 ◽  
Author(s):  
D. Saul ◽  
M. Ninkovic ◽  
M. Komrakova ◽  
L. Wolff ◽  
P. Simka ◽  
...  
Keyword(s):  
Bone Mass ◽  
Bone Healing ◽  
Plate Osteosynthesis ◽  
Estrogen Deficiency ◽  
Bone Volume ◽  
Negative Regulator ◽  
Ovariectomized Rats ◽  
Osteoporotic Bone ◽  
Cortical Volume ◽  
Age Related

Estrogen deficiency and aging are associated with osteoporosis, impaired bone healing, and lower cognitive performance. Close functional and physical connections occur between bone and the central nervous system. An anti-inflammatory drug, zileuton (which is an inhibitor of arachidonate 5-lipoxygenase), is known to have a positive effect on bone tissue repair and brain ischemia. We studied the effect of zileuton on osteopenic bone and its healing and on the genes considered to be crucial for the cross talks between bone and brain. Three-month-old Sprague-Dawley rats were ovariectomized or left untreated. After 8 wk, bilateral metaphyseal tibia osteotomy with plate osteosynthesis was performed in all rats. Ovariectomized rats were fed with food containing zileuton (1, 10, or 100 mg/kg body wt) for 5 wk. In tibiae, bone volume, callus and cortical volume, and gene expression of osteocalcin and alkaline phosphatase were enhanced by zileuton (10 or 100 mg); biomechanical properties and bone density were not changed. In femur, zileuton enlarged cortical volume distal and trabecular volume proximal, decreasing their density. The expression level of brain Sema3a, known to regulate bone mass positively, was downregulated after ovariectomy. In contrast, bone Sema4d, a negative regulator of bone mass, was upregulated in the tibia callus after ovariectomy, whereas zileuton treatment (10 or 100 mg) resulted in reverse effects. Here, we describe for the first time the expression of Rbbp4 mRNA and its increase in tibia after ovariectomy. Zileuton caused downregulation of Rbbp4 in the hippocampus and had an effect on bone healing, changed the expression of genes involved in cross talk between bones and brain, and may be a potent drug for further examination in estrogen deficiency-related dysfunction(s). NEW & NOTEWORTHY Zileuton, a 5-lipoxygenase inhibitor, increased bone volume, callus and cortical volume in osteotomized tibia, and trabecular and cortical volume in femur. Although the expression of Sema3a (positively regulating bone mass) in brain was downregulated and Sema4d (negatively regulating bone mass) was upregulated in tibia callus after ovariectomy, zileuton could counteract these effects. Rbbp4 (involved in age-related memory loss) was increased in tibia callus after ovariectomy.

Local and Systemic Morphofunctional Response of Osteoporotic Rabbits Bone Defect Following Implantation of Strontium Doped Biphasic Ceramic Granules

2017 ◽  
Vol 267 ◽  
pp. 124-131 ◽  
Author(s):  
Janis Zarins ◽  
Mara Pilmane ◽  
Elga Sidhoma ◽  
Ilze Salma ◽  
Janis Locs
Keyword(s):  
Bone Defect ◽  
Bone Healing ◽  
Statistical Difference ◽  
Healing Process ◽  
Bone Volume ◽  
Osteoporotic Bone ◽  
Sham Surgery ◽  
Local Bone ◽  
Group A ◽  
Biphasic Ceramic

Variety of different bone substitutive materials are synthetized to improve bone healing potentials in pathological bone conditions. Physiologically active molecules within biomaterials, can initiate expression level of biomarkers, regulating bone remodeling. Aim of our study was to analyze bone healing process in bone defects followed by implantation with 5% strontium substituted hydroxyapatite (HAP) /tricalcium phosphate (TCP) 70/30 granules (group A) or HAP/TCP biphasic ceramic granules without strontium substitution (group B), or sham surgery affected bone (group C) in osteoporotic rabbits’ femur. Tissue samples from contralateral intact left leg were used for evaluation of systemic effects after surgery. Changes of bone volume were measured and appearance of OPG, NFkB-105, OC, COL-1, BMP-2/4, MMP-2, TIMP-2, IL-1 and IL-10-positive osteocytes in osteoporotic rabbits’ bone defect were evaluated. No statistical difference between groups of trabecular bone volume was detected. All analyzed markers showed higher appearance of positive osteocytes in groups A and B with comparison to control left leg (p<0.05). Only NFkB105-positive cells showed important difference between sham surgery affected leg and control one (p=0.034). Numerous OPG-positive cells appeared in group A, while moderate number of them was found in groups B and C (p=0.025; p=0.027). Numerous to abundant OC-positive osteocytes were detected in group A, while moderate in group C (p=0.034). Statistical difference of rest biomarkers between groups was not detected. We concluded that implantation of biomaterials in osteoporotic bone improves local bone regenerative properties. However, the notable increase of OPG-containing cells proves the increase of osteoclastogenesis suppression and gives the evidence for renew of bone functionality.

scholarly journals Local SDF-1α application enhances the therapeutic efficacy of BMSCs transplantation in osteoporotic bone healing

Heliyon ◽  
2020 ◽  
Vol 6 (6) ◽  
pp. e04347
Author(s):  
Qian Liu ◽  
Yi Wen ◽  
Jun Qiu ◽  
Zhaoyichun Zhang ◽  
Zuolin Jin ◽  
...  
Keyword(s):  
Bone Healing ◽  
Therapeutic Efficacy ◽  
Osteoporotic Bone

scholarly journals A Standardized Critical Size Defect Model in Normal and Osteoporotic Rats to Evaluate Bone Tissue Engineered Constructs

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Livia Poser ◽  
Romano Matthys ◽  
Peter Schawalder ◽  
Simon Pearce ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Animal Model ◽  
Bone Healing ◽  
Wistar Rats ◽  
Critical Size ◽  
Osteoporotic Bone ◽  
Defect Model ◽  
Critical Size Defect ◽  
Titanium Screws ◽  
Fixation System

Tissue engineered constructs should be tested for their efficacy not only in normal but also in osteoporotic bone. The rat is an established animal model for osteoporosis and is used often for bone healing studies. In this study a defined and standardized critical size defect model in the rat suitable for screening new tissue engineered constructs in normal and osteoporotic bone is described and validated. Normal and ovariectomised Wistar rats received a unilateral middiaphyseal 5 mm defect in the femur, which was instrumented with a radiolucent PEEK plate fixed with angular stable titanium screws and left untreated. All animals were euthanized eight weeks after defect surgery and the bone healing was evaluated using radiographs, computed tomography measurements, and histology. The developed fixation system provided good stability, even in osteoporotic bone. The implants and ancillary instruments ensured consistent and facile placement of the PEEK plates. The untreated defects did not heal without intervention making the model a well-defined and standardized critical size defect model highly useful for evaluating tissue engineered solutions in normal and osteoporotic bone.

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