scholarly journals A metaphyseal fracture rat model for mechanistic studies of osteoporotic bone healing

2019 ◽  
Vol 37 ◽  
pp. 420-430 ◽  
Author(s):  
RMY Wong ◽  
U Thormann ◽  
MHV Choy ◽  
NY Chim ◽  
MCM Li ◽  
...  
Keyword(s):  
Bone Healing ◽  
Rat Model ◽  
Osteoporotic Bone ◽  
Mechanistic Studies ◽  
Metaphyseal Fracture

scholarly journals In Vivo Bone Effects of a Novel Bisphosphonate‐EP4a Conjugate Drug (C3) for Reversing Osteoporotic Bone Loss in an Ovariectomized Rat Model

JBMR Plus ◽  
2019 ◽  
Vol 3 (12) ◽  
Author(s):  
Zeeshan Sheikh ◽  
Gang Chen ◽  
Faik Al‐Jaf ◽  
Marion Thévenin ◽  
Kate Banks ◽  
...  
Keyword(s):  
Bone Loss ◽  
Rat Model ◽  
Ovariectomized Rat ◽  
Osteoporotic Bone

A Combination Treatment of Raloxifene and Vitamin D Enhances Bone-to-Tendon Healing of the Rotator Cuff in a Rat Model

2020 ◽  
Vol 48 (9) ◽  
pp. 2161-2169
Author(s):  
Dong Min Kim ◽  
In Kyoung Shim ◽  
Myung Jin Shin ◽  
Jae Hee Choi ◽  
Yu Na Lee ◽  
...  
Keyword(s):  
Vitamin D ◽  
Rotator Cuff ◽  
Bone Healing ◽  
Rat Model ◽  
Combination Treatment ◽  
Biomechanical Testing ◽  
Female Rats ◽  
Muscle Quality ◽  
Control Group ◽  
The Mean

Background: Tearing and degeneration of the rotator cuff at the tendon-to-bone junction are common in adults aged ≥50 years. Few studies have reported on the relationship between estrogen and the rotator cuff enthesis. In addition to preventing bone loss, selective estrogen receptor modulators have been shown to improve tendon and muscle quality. Purpose: To evaluate the effects of raloxifene (RLX) and vitamin D on rotator cuff tendon-to-bone healing in a rat model. Study Design: Controlled laboratory study. Methods: A total of 29 female rats (58 shoulders) were assigned to 4 groups: (1) control group, (2) ovariectomy (OVX)–only group, (3) no RLX group (OVX and rotator cuff repair [RCR]), and (4) RLX group (OVX, RCR, and RLX). Rats that did not undergo rotator cuff tear (RCT) surgery were divided into the control and OVX-only groups according to OVX surgery. Rats that underwent RCT surgery and RCR were divided into the no RLX and RLX groups according to RLX and vitamin D administration. An estrogen-deficient state was induced by OVX at 12 weeks of age. Bone mineral density (BMD) and trabecular bone characteristics were measured by micro–computed tomography, and healing of the tendon-to-bone junction was evaluated by biomechanical testing, histomorphometry, and micro–magnetic resonance imaging (MRI). Results: The mean final body weight (BW; 461.6 ± 47.3 g) of the OVX-only group was significantly higher and BMD (0.25 ± 0.07 g/cm3) was significantly lower ( P < .001) than the mean final BW (338.5 ± 35.1 g) and BMD (0.48 ± 0.05 g/cm3) of the control group. In contrast, the RLX group showed that the BW (369.6 ± 35.8 g) and BMD (0.41 ± 0.08 g/cm3) were not significantly different from the control group. The RLX group had a significantly higher histomorphometric total score (8.50 ± 1.05) than the no RLX group (4.83 ± 2.48). On biomechanical testing, the RLX group (29.7 ± 9.1 N) showed a significantly higher load to failure than the no RLX group (19.4 ± 8.8 N). On micro-MRI, the RLX group had a more homogeneous low signal and tendon continuity than the no RLX group. Conclusion: The combination treatment of RLX and vitamin D prevented a decrease in local BMD (greater tuberosity of the proximal humerus) and enhanced tendon-to-bone healing of the rotator cuff in a rat model. Clinical Relevance: This study induced an estrogen-deficient state similar to the human postmenopausal state and used drugs that are actually being prescribed in a clinical situation.

scholarly journals Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model

2017 ◽  
Vol 13 (5) ◽  
pp. 1827-1831 ◽  
Author(s):  
Şeref Ezirganli ◽  
Hakki Oğuz Kazancioğlu ◽  
Ahmet Hüseyin Acar ◽  
Hakan Özdemir ◽  
Emre Kuzu ◽  
...  
Keyword(s):  
Bone Healing ◽  
Rat Model

The Advantages of Bilateral Osteotomy Over Unilateral Osteotomy for Osteoporotic Bone Healing

2018 ◽  
Vol 103 (1) ◽  
pp. 80-94 ◽  
Author(s):  
M. Komrakova ◽  
J. Fiebig ◽  
D. B. Hoffmann ◽  
C. Krischek ◽  
W. Lehmann ◽  
...  
Keyword(s):  
Bone Healing ◽  
Osteoporotic Bone

Fracture Healing and Biomarker Expression in a Diabetic Zucker Rat Model

2014 ◽  
Vol 104 (5) ◽  
pp. 428-433 ◽  
Author(s):  
Javier La Fontaine ◽  
Nathan A. Hunt ◽  
Stacey Curry ◽  
Tyler Kearney ◽  
Daniel Jupiter ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fracture Healing ◽  
Bone Healing ◽  
Rat Model ◽  
Healing Process ◽  
Diabetic Rats ◽  
Vascular Endothelial ◽  
Delayed Healing ◽  
Endothelial Growth Factor

Background Persons with diabetes have a higher incidence of fractures compared with persons without diabetes. However, there is little published information concerning the deleterious effect of late-stage diabetes on fracture healing. There are no studies using animal models that evaluate the effect of advanced diabetes on fracture healing. The purpose of our study was to evaluate cytokine expression, specifically macrophage inflammatory protein 1 (MIP-1) and vascular endothelial growth factor, in fracture healing in a type 2 diabetes rat model. Methods We evaluated biomarker expression after femur fracture using a rat model. The two groups consisted of 24 Zucker diabetic rats (study group) and 12 Zucker lean rats (control group). An independent reviewer was used to assess delayed union. We evaluated serum samples 2, 4, 7, and 14 days after surgery for MIP-1, vascular endothelial growth factor, leptin, and other cytokine levels. Results At 3 weeks, Kaplan-Meier estimates showed that 45.8% of femur fractures in Zucker diabetic rats had healed, whereas 81.8% of those in Zucker lean rats had healed (P = .02). A logistic regression model to predict fast healing that included the three cytokines and diabetes status showed that the only factor achieving significance was MIP-1α. Vascular endothelial growth factor was the only biomarker to show significance compared with delayed healing. Conclusions These results confirm significant differences in biomarker expression between diabetic and nondiabetic rats during bone healing. The key factors for bone healing may appear early in the healing process, whereas differences in diabetes versus nondiabetes are seen later in the healing process. Increased levels of MIP-1α were associated with the likelihood of delayed healing.

scholarly journals The effects of chronic unloading and gap formation on tendon-to-bone healing in a rat model of massive rotator cuff tears

2013 ◽  
Vol 32 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Megan L. Killian ◽  
Leonardo Cavinatto ◽  
Shivam A. Shah ◽  
Eugene J. Sato ◽  
Samuel R. Ward ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Bone Healing ◽  
Rat Model ◽  
Rotator Cuff Tears ◽  
Gap Formation ◽  
Massive Rotator Cuff Tears

A Synthetic Graft With Multilayered Co-Electrospinning Nanoscaffolds for Bridging Massive Rotator Cuff Tear in a Rat Model

2020 ◽  
Vol 48 (8) ◽  
pp. 1826-1836 ◽  
Author(s):  
Shuang Cong ◽  
Yaying Sun ◽  
Jinrong Lin ◽  
Shaohua Liu ◽  
Jiwu Chen
Keyword(s):  
Rotator Cuff ◽  
Bone Healing ◽  
Rat Model ◽  
Layered Structure ◽  
Tendon Healing ◽  
Collagen I ◽  
Control Group ◽  
Massive Rotator Cuff Tear ◽  
Cuff Tear ◽  
Collagen Ii

Background: Graft bridging is used in massive rotator cuff tear (MRCT); however, the integration of graft-tendon and graft-bone is still a challenge. Hypothesis: A co-electrospinning nanoscaffold of polycaprolactone (PCL) with an “enthesis-mimicking” (EM) structure could bridge MRCT, facilitate tendon regeneration, and improve graft-bone healing. Study Design: Controlled laboratory study. Methods: First, we analyzed the cytocompatibility of the electrospinning nanoscaffolds, including aligned PCL (aPCL), nonaligned PCL (nPCL), aPCL–collagen I, nPCL–collagen II, and nPCL-nanohydroxyapatite (nHA). Second, for the EM condition, nPCL–collagen II and nPCL-nHA were electrospun layer by layer at one end of the aPCL–collagen I; for the control condition, the nPCL was electrospun on the aPCL. In 40 mature male rats, resection of both the supraspinatus and infraspinatus tendons was performed to create MRCT, and the animals were divided randomly into EM and control groups. In both groups, one end of the layered structure was fixed on the footprint of the rotator cuff, whereas the other end of the layered structure was sutured with the tendon stump. The animals were euthanized for harvesting of tissues for histologic and biomechanical analysis at 4 weeks or 8 weeks postoperatively. Results: All scaffolds showed good cytocompatibility in vitro. The graft-tendon tissue in the EM group had more regularly arranged cells, denser tissue, a significantly higher tendon maturing score, and more birefringence compared with the control group at 8 weeks after operation. Newly formed fibrocartilage could be observed at the graft-bone interface in both groups by 8 weeks, but the EM group had a higher graft-bone healing score and significantly more newly formed fibrocartilage than the control group. An enthesis-like structure with transitional layers was observed in the EM group at 8 weeks. Biomechanically, the values for maximum failure load and stiffness of the tendon-graft-bone complex were significantly higher in the EM group than in the control group at 8 weeks. Conclusion: The co-electrospinning nanoscaffold of aPCL–collagen I could be used as a bridging graft to improve early graft-tendon healing for MRCT in a rat model and enhance early enthesis reconstruction in combination with a multilayered structure of nPCL–collagen II and nPCL-nHA. Clinical Relevance: We constructed a graft to bridge MRCT, enhance graft-tendon healing and graft-bone healing, and reconstruct the enthesis structure.

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