Purification and characterization of recombinant Escherichia coli-expressed Pichia etchellsii ?-glucosidase II with high hydrolytic activity on sophorose

2004 ◽  
Vol 66 (5) ◽  
pp. 527-535 ◽  
Author(s):  
Yukti Bhatia ◽  
Saroj Mishra ◽  
Virendra S. Bisaria
Keyword(s):  
Escherichia Coli ◽  
Hydrolytic Activity ◽  
Recombinant Escherichia Coli ◽  
Purification And Characterization ◽  
Glucosidase Ii ◽  
Pichia Etchellsii

scholarly journals Purification and characterization of human interleukin-1beta expressed in recombinant Escherichia coli

1986 ◽  
Vol 160 (3) ◽  
pp. 491-497 ◽  
Author(s):  
Paul WINGFIELD ◽  
Mark PAYTON ◽  
Jean TAVERNIER ◽  
Marjory BARNES ◽  
Alan SHAW ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Purification And Characterization ◽  
Interleukin 1Beta

Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application

Biologia ◽  
2009 ◽  
Vol 64 (6) ◽  
Author(s):  
Yue-Hong Wang ◽  
Yu Jiang ◽  
Zuo-Ying Duan ◽  
Wei-Lan Shao ◽  
Hua-Zhong Li
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Optimal Condition ◽  
Industrial Application ◽  
Conversion Rate ◽  
Hydrolytic Activity ◽  
Thermoanaerobacter Ethanolicus ◽  
Transglycosylation Activity

AbstractIn this study, a new α-glucosidase gene from Thermoanaerobacter ethanolicus JW200 was cloned and expressed in Escherichia coli by a novel heat-shock vector pHsh. The recombinant α-glucosidase exhibited its maximum hydrolytic activity at 70°C and pH 5.0∼5.5. With p-nitrophenyl-α-D-glucoside as a substrate and under the optimal condition (70°C, pH 5.5), K m and V max of the enzyme was 1.72 mM and 39 U/mg, respectively. The purified α-glucosidase could hydrolyze oligosaccharides with both α-1,4 and α-1,6 linkages. The enzyme also had strong transglycosylation activity when maltose was used as sugar donor. The transglucosylation products towards maltose are isomaltose, maltotriose, panose, isomaltotriose and tetrasaccharides. The enzyme could convert 400 g/L maltose to oligosaccharides with a conversion rate of 52%, and 83% of the oligosaccharides formed were prebiotic isomaltooligosaccharides (containing isomaltose, panose and isomaltotriose).

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