Cloning and expression of a trehalose synthase from Pseudomonas stutzeri CJ38 in Escherichia coli for the production of trehalose

2005 ◽  
Vol 68 (2) ◽  
pp. 213-219 ◽  
Author(s):  
Jin-Ho Lee ◽  
Kwang-Ho Lee ◽  
Chang-Gyeom Kim ◽  
Se-Young Lee ◽  
Geun-Joong Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Stutzeri ◽  
Cloning And Expression ◽  
Trehalose Synthase

Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose

2014 ◽  
Vol 60 (9) ◽  
pp. 599-604 ◽  
Author(s):  
Tengfei Wang ◽  
Shiru Jia ◽  
Kun Dai ◽  
Hongjuan Liu ◽  
Ruiming Wang
Keyword(s):  
Escherichia Coli ◽  
High Pressure ◽  
Pseudomonas Putida ◽  
Scale Up ◽  
Activity Analysis ◽  
Cloning And Expression ◽  
Pseudomonas Putida Kt2440 ◽  
Trehalose Synthase ◽  
E Coli ◽  
Optimal Activity

Trehalose synthase (TreS) is considered to be a potential biocatalyst for trehalose production. We aimed to scale-up produce the TreS protein in Escherichia coli and further investigate the bioconversion capacity of TreS. The treS gene from Pseudomonas putida KT2440 was amplified and expressed in E. coli BL21 (DE3). The recombinant TreS showed a molecular mass of 67 kDa. Activity analysis suggested that TreS had optimal activity at a temperature of 55 °C, a pH of 7.4, with a substrate concentration of 30%. High-pressure liquid chromatography results indicated that this enzyme had the ability to catalyze 59% maltose into trehalose, with about 5.1% glucose as by-product. Purification analysis showed that trehalose crystals with a purity of 98% were obtained by cooling trehalose solution. The TreS from P. putida KT2440 might be a candidate for trehalose production. Further study is needed to improve the trehalose conversion rate.

scholarly journals A novel chitosanase from Bacillus sp. Strain K17: Gene cloning and expression in Escherichia coli

2002 ◽  
Vol 2 (1) ◽  
pp. 227-228 ◽  
Author(s):  
R. Yatsunami ◽  
Y. Sakihama ◽  
M. Suzuki ◽  
T. Fukazawa ◽  
S. Shimizu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cloning ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Bacillus Sp ◽  
Expression In Escherichia Coli

scholarly journals Molecular cloning and expression of the porcine S14R gene in Escherichia coli

2013 ◽  
Vol 12 (4) ◽  
pp. 4405-4412
Author(s):  
Y.J. Guo ◽  
G.Z. Liu ◽  
C.M. Wang ◽  
Y.Y. Wang ◽  
H.J. Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Cloning ◽  
Cloning And Expression

scholarly journals Escherichia coli as a genetic tool

1985 ◽  
Vol 95 (3) ◽  
pp. 611-618
Author(s):  
Naomi Datta
Keyword(s):  
Escherichia Coli ◽  
Academic Research ◽  
Genetically Engineered ◽  
Cloning And Expression ◽  
Biological Research ◽  
New Techniques ◽  
E Coli ◽  
Genetic Tool ◽  
The Past ◽  
Made In

SUMMARYThe study of Escherichia coli and its plasmids and bacteriophages has provided a vast body of genetical information, much of it relevant to the whole of biology. This was true even before the development of the new techniques, for cloning and analysing DNA, that have revolutionized biological research during the past decade. Thousands of millions of dollars are now invested in industrial uses of these techniques, which all depend on discoveries made in the course of academic research on E. coli. Much of the background of knowledge necessary for the cloning and expression of genetically engineered information, as well as the techniques themselves, came from work with this organism.

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