Phenylalanine ammonia-lyase and stilbene synthase gene expression in rolB transgenic cell cultures of Vitis amurensis

2009 ◽  
Vol 82 (4) ◽  
pp. 647-655 ◽  
Author(s):  
K. V. Kiselev ◽  
A. S. Dubrovina ◽  
V. P. Bulgakov
Keyword(s):  
Gene Expression ◽  
Cell Cultures ◽  
Phenylalanine Ammonia Lyase ◽  
Stilbene Synthase ◽  
Vitis Amurensis ◽  
Transgenic Cell ◽  
Stilbene Synthase Gene ◽  
Transgenic Cell Cultures

Metabolic regulation of ?-amylase gene expression in transgenic cell cultures of rice (Oryza sativa L.)

1993 ◽  
Vol 23 (4) ◽  
pp. 737-747 ◽  
Author(s):  
Ning Huang ◽  
John Chandler ◽  
Bruce R. Thomas ◽  
Nozomu Koizumi ◽  
Raymond L. Rodriguez
Keyword(s):  
Gene Expression ◽  
Oryza Sativa ◽  
Cell Cultures ◽  
Metabolic Regulation ◽  
Oryza Sativa L ◽  
Amylase Gene ◽  
Transgenic Cell ◽  
Transgenic Cell Cultures

scholarly journals Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression

2006 ◽  
Vol 19 (10) ◽  
pp. 1103-1112 ◽  
Author(s):  
Laurent Laquitaine ◽  
Eric Gomès ◽  
Julie François ◽  
Chloé Marchive ◽  
Stéphanie Pascal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Botrytis Cinerea ◽  
Molecular Basis ◽  
Stilbene Synthase ◽  
Gus Activity ◽  
Cell Suspensions ◽  
Type I ◽  
Lipid Transfer ◽  
Tobacco Plants ◽  
Stilbene Synthase Gene

Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-glucuronidase (GUS) reporter gene, and introduced in tobacco plants and grapevine cell suspensions using Agrobacterium spp. Single MYB- and the W-boxes identified on the 0.250-kbp fragment were sufficient to induce GUS activity in transgenic tobacco plants after transient expression of MYB and WRKY. Ergosterol, a nonspecific fungal elicitor, induced GUS activity in transgenic grapevine cell suspensions transformed with the 1,420- and 750-bp promoter containing a palindromic arrangement of two Wboxes but not the 650- or 250-bp fragment, where only one W-box was present. Moreover, ergosterol triggered WRKY, VvLTP1, and stilbene synthase gene expression in grape plantlets and enhanced protection against Botrytis cinerea. The molecular basis of ergosterol-induced protection is discussed.

Transgenic cell cultures that synthesize neurotrophic factors and the possibility of therapeutic use of its cells

2012 ◽  
Vol 43 (1) ◽  
pp. 60-65
Author(s):  
G. V. Pavlova ◽  
N. N. Kanaykina ◽  
D. Yu. Panteleev ◽  
V. E. Okhotin ◽  
A. V. Revishchin
Keyword(s):  
Cell Cultures ◽  
Neurotrophic Factors ◽  
Therapeutic Use ◽  
Transgenic Cell ◽  
Transgenic Cell Cultures

A new strategy to enhance the biosynthesis of trans -resveratrol by overexpressing stilbene synthase gene in elicited Vitis vinifera cell cultures

2017 ◽  
Vol 113 ◽  
pp. 141-148 ◽  
Author(s):  
Mingyu Chu ◽  
M.A. Pedreño ◽  
Nuria Alburquerque ◽  
Lydia Faize ◽  
Lorenzo Burgos ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Cell Cultures ◽  
Stilbene Synthase ◽  
New Strategy ◽  
Stilbene Synthase Gene
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