The effect of salicylic acid on phenylalanine ammonia-lyase and stilbene synthase gene expression in Vitis amurensis Cell Culture

2010 ◽  
Vol 57 (3) ◽  
pp. 415-421 ◽  
Author(s):  
K. V. Kiselev ◽  
A. S. Dubrovina ◽  
G. A. Isaeva ◽  
Y. N. Zhuravlev
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Salicylic Acid ◽  
Phenylalanine Ammonia Lyase ◽  
Stilbene Synthase ◽  
Vitis Amurensis ◽  
Stilbene Synthase Gene

scholarly journals Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression

2006 ◽  
Vol 19 (10) ◽  
pp. 1103-1112 ◽  
Author(s):  
Laurent Laquitaine ◽  
Eric Gomès ◽  
Julie François ◽  
Chloé Marchive ◽  
Stéphanie Pascal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Botrytis Cinerea ◽  
Molecular Basis ◽  
Stilbene Synthase ◽  
Gus Activity ◽  
Cell Suspensions ◽  
Type I ◽  
Lipid Transfer ◽  
Tobacco Plants ◽  
Stilbene Synthase Gene

Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-glucuronidase (GUS) reporter gene, and introduced in tobacco plants and grapevine cell suspensions using Agrobacterium spp. Single MYB- and the W-boxes identified on the 0.250-kbp fragment were sufficient to induce GUS activity in transgenic tobacco plants after transient expression of MYB and WRKY. Ergosterol, a nonspecific fungal elicitor, induced GUS activity in transgenic grapevine cell suspensions transformed with the 1,420- and 750-bp promoter containing a palindromic arrangement of two Wboxes but not the 650- or 250-bp fragment, where only one W-box was present. Moreover, ergosterol triggered WRKY, VvLTP1, and stilbene synthase gene expression in grape plantlets and enhanced protection against Botrytis cinerea. The molecular basis of ergosterol-induced protection is discussed.

Stress enhances the gene expression and enzyme activity of phenylalanine ammonia-lyase and the endogenous content of salicylic acid to induce flowering in pharbitis

2014 ◽  
Vol 171 (11) ◽  
pp. 895-902 ◽  
Author(s):  
Kaede C. Wada ◽  
Kaori Mizuuchi ◽  
Aya Koshio ◽  
Kentaro Kaneko ◽  
Toshiaki Mitsui ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Salicylic Acid ◽  
Phenylalanine Ammonia Lyase

Salicylic acid-induced autoexcision of the nptii marker gene in elite tomato plants transformed with a stilbene synthase gene

2008 ◽  
Vol 136 ◽  
pp. S242
Author(s):  
Binggang Ma ◽  
Xiaoyu Duan ◽  
Luxia Zhang ◽  
Chao Ma ◽  
Qingnan Hao ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Marker Gene ◽  
Stilbene Synthase ◽  
Tomato Plants ◽  
Stilbene Synthase Gene

scholarly journals Elicitor induction of defence genes and reduction of bacterial canker in kiwifruit

2017 ◽  
Vol 70 ◽  
pp. 272-284 ◽  
Author(s):  
K.V. Wurms ◽  
E. Gould ◽  
A. Ah Chee ◽  
J. Taylor ◽  
B. Curran ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Pseudomonas Syringae ◽  
Phenylalanine Ammonia Lyase ◽  
Induced Response ◽  
Bacterial Canker ◽  
Actinidia Chinensis ◽  
Interacting Protein ◽  
Defence Genes ◽  
Control Options

Pseudomonas syringae pv. actinidiae (Psa), which causes bacterial canker, is the most serious global pathogen of kiwifruit. Like most bacterial pathogens, control options are limited, but elicitors can reduce disease significantly, particularly those that induce the salicylic acid (SA) pathway. Acibenzolar-S-methyl (ASM), a SA analogue, is one of the most effective elicitors for Psa control. In this study, real-time PCR (qPCR) was used to measure the expression of 18 putative defence genes in Actinidia chinensis var. chinensis ‘Hort16A’ in response to Psa and ASM. Application of ASM led to up-regulation of RPM1 interacting protein 4 (RIN4), phenylalanine ammonia lyase (PAL), a hypersensitivity-induced response protein (HIRP), and β-1,3-glucosidase. Expression of PAL and HIRP was further enhanced when elicitor application and Psa-inoculation were combined. Elevated gene expression was correlated with decreased disease expression, and supports the hypothesis that elicitor-treated plants are primed to react more rapidly and/or strongly to pathogens.

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