Cloning, characterization, and antifungal activity of an endo-1,3-β-d-glucanase from Streptomyces sp. S27

AbstractVal-Geninthiocin (2), a new member of thiopeptide antibiotics, was isolated from the mycelium of Streptomyces sp. RSF18, along with the closely related geninthiocin (1) and the macrolide, chalcomycin. By intensive NMR and MS studies, Val-geninthiocin (2) was identified as desoxygeninthiocin, a thiopeptide, containing several oxazole and thiazole units and a number of unusual amino acids. Compound 2 shows potent activity against Gram-positive bacteria and minor antifungal activity, while it is not effective against Gram-negative bacteria or microalgae. Here we describe the fermentation, isolation and structure elucidation as well as the biological activity of 2.

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Purification and characterization of salvianolic acid B from Streptomyces sp. M4 possessing antifungal activity against fungal phytopathogens

Microbiological Research ◽

10.1016/j.micres.2020.126478 ◽

2020 ◽

Vol 237 ◽

pp. 126478 ◽

Cited By ~ 1

Author(s):

Manish Sharma ◽

Rajesh Kumari Manhas

Keyword(s):

Antifungal Activity ◽

Salvianolic Acid B ◽

Purification And Characterization ◽

Streptomyces Sp ◽

Salvianolic Acid ◽

Fungal Phytopathogens

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Evaluation of antifungal activity of novel marine actinomycete, Streptomyces sp. AA13 isolated from sediments of Lake Oubeira (Algeria) against Candida albicans

African Journal of Microbiology Research ◽

10.5897/ajmr2013.7765 ◽

2016 ◽

Vol 10 (6) ◽

pp. 156-171 ◽

Cited By ~ 1

Author(s):

Ayari Adel ◽

Morakchi Houda ◽

Kirane Gacemi Djamila

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Streptomyces Sp ◽

Marine Actinomycete

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Actinobacteria mediated synthesis of gold nanoparticles using Streptomyces sp. VITDDK3 and its antifungal activity

Materials Letters ◽

10.1016/j.matlet.2012.11.125 ◽

2013 ◽

Vol 93 ◽

pp. 360-362 ◽

Cited By ~ 24

Author(s):

Jannu Vinay Gopal ◽

Mohankumar Thenmozhi ◽

Krishnan Kannabiran ◽

Govindasamy Rajakumar ◽

Kanayairam Velayutham ◽

...

Keyword(s):

Gold Nanoparticles ◽

Antifungal Activity ◽

Streptomyces Sp

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Volatile Organic Compounds (VOCs) Produced By The Rhizosphere Actinobacteria Streptomyces Sp. and Amycolatopsis Sp. Strains and Their Antifungal Activity Against Colletotrichum Musae

10.21203/rs.3.rs-509649/v1 ◽

2021 ◽

Author(s):

Maria Vieira de Brito ◽

Wéverson L. Fonseca ◽

Jair Mafezoli ◽

Francisco Geraldo Barbosa ◽

Fátima Miranda Nunes ◽

...

Keyword(s):

Antifungal Activity ◽

Organic Compounds ◽

Morphological Structure ◽

Dimethyl Disulfide ◽

Culture Conditions ◽

Fungal Cell ◽

Streptomyces Sp ◽

Volatile Organic ◽

Antifungal Potential ◽

Colletotrichum Musae

Abstract Herein is reported the study on the antifungal potential of VOCs produced by actinobacterial strains from the rhizosphere of Caatinga plants. Twenty strains were screened for their ability to produce VOCs with growth inhibition (GI) potential against the phytopathogen fungus Colletotrichum musae . The most active VOCs were produced by Streptomyces sp. (ACTB-77) and Amycolatopsis sp. (ACTB-290). The effect of VOCs from ACTB-77 and ACTB-290 on the morphological structure of the fungus hyphae was investigated by SEM and revealed significant deformation of the fungal cell filaments, especially in the case of ACTB-290. VOCs produced by these two actinobacteria strains and the fungus under axenic and co-culture conditions were investigated using HS-SPME-GCMS combined approaches, and all data were analyzed by statistical methods. Most of the mVOCs were identified, representing 92-100% of total peaks areas. Linalool (38.53±5.19%) and geosmin (17.25±2.55%) were the major VOCs constituents from ACTB-77, while dimethyl disulfide (48.49±3.48%) and dimethyl trisulfide (21.14±3.50%) were the major VOCs compounds produced by ACTB-290. Statistical analyses of data from co-cultures showed that the VOCs profile of the experiment involving ACTB-77 was more influenced by the fungal VOCs while the experiment with ACTB-290 was more influence by compounds from the actinobacteria.

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Evaluation of Antifungal, Hemolytic and Cytotoxic Potential of Ethyl Acetate Extract of a New Marine Streptomyces sp. AIAH-10

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v19i1.29235 ◽

2016 ◽

Vol 19 (1) ◽

pp. 37-43

Author(s):

Md Anwarul Haque ◽

Ashish Kumar Sarker ◽

Md Ajijur Rahman ◽

Md Aktar Uzzaman Chouduri ◽

Md Anwar Ul Islam

Keyword(s):

Antifungal Activity ◽

Mortality Rate ◽

Ethyl Acetate ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Bioactive Molecules ◽

Diffusion Method ◽

Small Scale ◽

Dilution Method ◽

Streptomyces Sp

To face newly generated diseases, search for new, safe and effective bioactive molecules is highly warranted. The marine microbial flora may be a potential source of such molecules. The present study was designed to isolate marine microorganisms (AIAH-1 to AIAH-29) from the soil of mangrove forest Sundarbans, Bangladesh by serial dilution method using isolation media. Among them, AIAH-10 was selected for further study due to its promising antibacterial activity (done by streak plate and plug technique method) against a series of pathogenic bacteria. On the basis of morphological, cultural and biochemical analysis, the strain AIAH-10 belongs to Streptomyces sp. Secondary metabolites of the strain was obtained by small scale fermentation process. Antifungal activity of the extracts was performed by disc diffusion method. The crude ethyl acetate extract (50µg/disc) showed significant antifungal activity against Aspergillus niger, Candida albicans and Saccharromyces cerevaceae (14, 12 and 10 mm zone of inhibition respectively). No hemolytic activity was found of the extracts towards the human erythrocytes. During cytotoxic study against brine shrimp nauplii (Artemia salina), a dose dependent mortality rate was observed. A 100% larva mortality rate was recorded in 40µg/ml and more where as LC50 was found to be 6.61µg/ml.Bangladesh Pharmaceutical Journal 19(1): 37-43, 2016

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