scholarly journals A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

2020 ◽  
Vol 11 ◽  
Author(s):  
Yuanyuan Wei ◽  
Yankun Zhao ◽  
Dengbo Zhou ◽  
Dengfeng Qi ◽  
Kai Li ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Broad Spectrum ◽  
Plant Resistance ◽  
Banana Plant ◽  
Streptomyces Sp ◽  
Race 4

Synthesis and Antifungal Activities of Some 2,6-Bis-(Un)Substituted Phenoxymethylpyridines

2010 ◽  
Vol 65 (7-8) ◽  
pp. 433-436
Author(s):  
Hui Xu ◽  
Huan Qu
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Fusarium Graminearum ◽  
Broad Spectrum ◽  
Alternaria Alternata ◽  
Phytopathogenic Fungi ◽  
Alternaria Brassicae ◽  
Antifungal Activities

Several 2,6-bis-(un)substituted phenoxymethylpyridines were synthesized and evaluated in vitro against Fusarium graminearum, Helminthosporium sorokinianum, Alternaria brassicae, Alternaria alternata, and Fusarium oxysporum f. sp. vasinfectum. Among all derivatives, compound 3 a exhibited a broad-spectrum antifungal activity against the five phytopathogenic fungi.

scholarly journals Taxonomy and Broad-Spectrum Antifungal Activity of Streptomyces sp. SCA3-4 Isolated From Rhizosphere Soil of Opuntia stricta

2019 ◽  
Vol 10 ◽  
Author(s):  
Dengfeng Qi ◽  
Liangping Zou ◽  
Dengbo Zhou ◽  
Yufeng Chen ◽  
Zhufen Gao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Broad Spectrum ◽  
Rhizosphere Soil ◽  
Streptomyces Sp

Biological control of Fusarium oxysporum f. sp. cubense Tropical Race 4 in banana plantlets using newly isolated Streptomyces sp. WHL7 from marine soft coral

Plant Disease ◽  
2021 ◽  
Author(s):  
Jie Wang ◽  
Bingyu Cai ◽  
Kai Li ◽  
Yankun Zhao ◽  
Chunyu Li ◽  
...  
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Soft Coral ◽  
Fermentation Broth ◽  
Cell Integrity ◽  
Streptomyces Sp ◽  
Promising Strategy ◽  
Almost All ◽  
Race 4 ◽  
High Antifungal Activity

Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is a disastrous fungal disease. Foc tropical race 4 (Foc TR4) infects almost all banana cultivars. Use of chemical fungicides caused seriously environment pollution. Biological control with antagonistic microbes is a promising strategy for controlling Foc TR4. Here, strain WHL7 isolated from marine soft coral exhibited a high antifungal activity against Foc TR4. Based on the morphological and physicochemical profiles as well as the phylogenetic tree, the strain was assigned to Streptomyces sp.. Fermentation broth of Streptomyces sp. WHL7 significantly increased the resistance of banana plantlets to Foc TR4 in the pot experiment. Analysis of antifungal mechanism showed that strain WHL7 extracts inhibited spore germination and mycelial growth of Foc TR4, and destroyed cell integrity and ultrastructure. Hence, Streptomyces sp. WHL7 is an important bioresource for exploring novel natural products and biofertilizer to manage Foc TR4.

Endophytic Bacillus subtilis TR21 Improves Banana Plant Resistance to Fusarium oxysporum f. sp. cubense and Promotes Root Growth by Upregulating the Jasmonate and Brassinosteroid Biosynthesis Pathways

2021 ◽  
Author(s):  
Yunhao Sun ◽  
Bingzhi Huang ◽  
Ping Cheng ◽  
Chunji Li ◽  
Yanhong Chen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Root Growth ◽  
Fusarium Oxysporum ◽  
Plant Resistance ◽  
Growth Period ◽  
Subtilis Strain ◽  
Banana Plant ◽  
Musa Spp ◽  
Transcriptional Reprogramming ◽  
Race 1

The banana (Musa spp.) industry experiences dramatic annual losses from Fusarium wilt of banana (FWB) disease, which is caused by the fungus Fusarium oxysporum f. sp. cubense (FOC). Pisang Awak banana Fenza No. 1 (Musa spp. cv. Fenza No. 1), a major banana cultivar with high resistance to FOC race 4, is considered to be ideal for growth in problematic areas. However, Fenza No. 1 is still affected by FOC race 1 in the field. TR21 is an endophytic Bacillus subtilis strain isolated from orchids (Dendrobium sp.). Axillary spraying of banana plants with TR21 controls FWB, decreasing the growth period and increasing yields in the field. In this study, we established that TR21 increases root growth in different monocotyledonous plant species. By axillary inoculation, TR21 induced a similar transcriptomic change as that induced by FOC race 1 but also upregulated the biosynthetic pathways for the phytohormones brassinosteroid and jasmonate in Fenza No. 1 root tissues, indicating that TR21 increases FWB resistance, shortens growth period, and increases yield of banana by inducing specific transcriptional reprogramming and modulating phytohormone levels. These findings will contribute to the identification of candidate genes related to plant resistance against fungi in a non-model system and facilitate further study and exploitation of endophytic biocontrol agents.

Aureoverticillactam, a potent antifungal macrocyclic lactam from Streptomyces aureoverticillatus NH6, generates calcium dyshomeostasis induced cell apoptosis via the phospholipase C pathway in Fusarium oxysporum f. sp. cubense race 4

2021 ◽  
Author(s):  
Lanying Wang ◽  
Yunfei Zhang ◽  
Deyou Yang ◽  
Shujing Zhang ◽  
Dandan Han ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Phospholipase C ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
High Efficiency ◽  
Hyphal Growth ◽  
Complex Iii ◽  
Calcium Dependent ◽  
Race 4

Extensive efforts have been devoted to discover new bio-fungicides of high efficiency for control of Fusarium oxysporum f. sp. cubense race 4 (Foc4), a catastrophic soil-borne phytopathogen causing banana fusarium wilt worldwide. In this paper, aureoverticillactam (YY3) was verified to possess a potent antifungal activity against Foc4 for the first time, with the EC50 values of 20.80 μg/ml against hyphal growth, and 12.62 μg/ml against spore germination. To provide insight into its action mechanism, the cellular ultrastructures of Foc4 was observed with YY3 treatment and the results revealed that YY3 led to cell wall thinning, mitochondrial deformities, apoptotic degradation of the subcellular fractions and entocyte leakage. Consistent with these variations, the enhanced permeability of cell membrane and mitochondrial membrane also occurred after YY3 treatment. At the enzymatic level, the activity of mitochondrial complex III, as well as the ATP synthase, was significantly suppressed by YY3 at a concentration above 12.50 μg/ml. Moreover, YY3 elevated the cytosolic Ca2+ level to promote the mitochondrial ROS production. The cell apoptosis also occurred as expected. At the transcriptome level, key genes involved in phosphatidylinositol signaling pathway were significantly impacted, with the expression level of Plc1 elevated by approximately 4 folds. The expression levels of two apoptotic genes, casA1 and casA2, were also significantly elevated by YY3. Of note, the activation of phospholipase C was observed by YY3 treatment in Foc4. These findings indicate that YY3 exerts its antifungal activity via activating the phospholipase C-calcium-dependent ROS signaling pathway, which makes it a great potential bio-fungicide

scholarly journals Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4)

2019 ◽  
Vol 26 (4) ◽  
pp. 196
Author(s):  
Listya Utami Karmawan ◽  
Fenny Martha Dwivany ◽  
Rizkita Rachmi Esyanti ◽  
I Nyoman Pugeg Aryantha
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Detection ◽  
Previous Method ◽  
Fungal Genome ◽  
Pcr Analysis ◽  
Banana Plant ◽  
Fungal Dna ◽  
Pre Treatment ◽  
Race 4 ◽  
Treatment Step

Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.

scholarly journals Cu(II) complexes of 2-(diphenylmethylene)hydrazinecarboxamide derivatives: Synthesis, characterization and antifungal activity against Fusarium oxysporum f. sp. cubense tropical race 4

2017 ◽  
Vol 10 ◽  
pp. S3493-S3500 ◽  
Author(s):  
Yi Chen Chan ◽  
Abdussalam Salhin Mohamed Ali ◽  
Baharuddin Salleh ◽  
Hafizi Rosli ◽  
Ching Kheng Quah
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Race 4

scholarly journals The M35 Metalloprotease Effector FocM35_1 Is Required for Full Virulence of Fusarium oxysporum f. sp. cubense Tropical Race 4

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 670
Author(s):  
Xiaoxia Zhang ◽  
Huoqing Huang ◽  
Bangting Wu ◽  
Jianghui Xie ◽  
Altus Viljoen ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Chitinase Activity ◽  
Effector Proteins ◽  
Knockout Mutant ◽  
Lesion Formation ◽  
Banana Plant ◽  
Pathogen Virulence ◽  
Fusarium Wilt Of Banana ◽  
Banana Leaves ◽  
Race 4

Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) causes Fusarium wilt of banana, the most devastating disease on a banana plant. The genome of Foc TR4 encodes many candidate effector proteins. However, little is known about the functions of these effector proteins on their contributions to disease development and Foc TR4 virulence. Here, we discovered a secreted metalloprotease, FocM35_1, which is an essential virulence effector of Foc TR4. FocM35_1 was highly upregulated during the early stages of Foc TR4 infection progress in bananas. The FocM35_1 knockout mutant compromised the virulence of Foc TR4. FocM35_1 could interact with the banana chitinase MaChiA, and it decreased banana chitinase activity. FocM35_1 induced cell death in Nicotiana benthamiana while suppressing the INF1-induced hypersensitive response (HR), and its predicted enzymatic site was required for lesion formation and the suppression to INF1-induced HR on N. benthamiana leaves. Importantly, treatment of banana leaves with recombinant FocM35_1 accelerates Foc TR4 infection. Collectively, our study provides evidence that metalloprotease effector FocM35 seems to contribute to pathogen virulence by inhibiting the host immunity.

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