Production and characterization of a milk-clotting enzyme from Aspergillus oryzae MTCC 5341

2009 ◽  
Vol 85 (6) ◽  
pp. 1849-1859 ◽  
Author(s):  
Kurutahalli S. Vishwanatha ◽  
A. G. Appu Rao ◽  
Sridevi Annapurna Singh
Keyword(s):  
Aspergillus Oryzae ◽  
Milk Clotting ◽  
Milk Clotting Enzyme

A novel milk-clotting enzyme from Aspergillus oryzae and A. luchuensis is an aspartic endopeptidase PepE presumed to be a vacuolar enzyme

2022 ◽  
Author(s):  
Yoko Takyu ◽  
Taro Asamura ◽  
Ayako Okamoto ◽  
Hiroshi Maeda ◽  
Michio Takeuchi ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Proteolytic Activities ◽  
Homologous Enzyme ◽  
Traditional Fermentation ◽  
Enzyme Source ◽  
Cheese Production ◽  
Milk Clotting Activity ◽  
Aspartic Endopeptidase

Abstract Aspergillus oryzae RIB40 has 11 aspartic endopeptidase genes. We searched for milk-clotting enzymes based on the homology of the deduced amino acid sequence with chymosins. As a result, we identified a milk-clotting enzyme in A. oryzae. We expected other Aspergillus species to have a homologous enzyme with milk-clotting activity, and we found the most homologous aspartic endopeptidase from A. luchuensis had milk-clotting activity. Surprisingly, two enzymes were considered as vacuole enzymes according to a study on A. niger proteases. The two enzymes from A. oryzae and A. luchuensis cleaved a peptide between the 105Phe-106Met bond in κ-casein, similar to chymosin. Although both enzymes showed proteolytic activity using casein as a substrate, the optimum pH values for milk-clotting and proteolytic activities were different. Furthermore, the substrate specificities were highly restricted. Therefore, we expected that the Japanese traditional fermentation agent, koji, could be used as an enzyme source for cheese production.

Optimization of the production and characterization of milk-clotting enzyme from Bacillus subtilis isolated from marine sponge

2016 ◽  
Vol 15 (3) ◽  
pp. 158 ◽  
Author(s):  
HalaR Wehaidy ◽  
MohamedA Abdel-Naby ◽  
WafaaG Shousha ◽  
MohammedI.Y. El Mallah ◽  
MichaelM Shawky
Keyword(s):  
Bacillus Subtilis ◽  
Marine Sponge ◽  
Milk Clotting ◽  
Milk Clotting Enzyme

Production and characterization of milk-clotting enzyme from Bacillus amyloliquefaciens JNU002 by submerged fermentation

2011 ◽  
Vol 234 (3) ◽  
pp. 415-421 ◽  
Author(s):  
Zhongyang Ding ◽  
Wangfei Wang ◽  
Boda Wang ◽  
Anran Ouyang ◽  
Siwei Xiao ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Bacillus Amyloliquefaciens ◽  
Milk Clotting ◽  
Milk Clotting Enzyme

Purification and partial characterization of milk-clotting enzyme extracted from glutinous rice wine mash liquor

2009 ◽  
Vol 26 (5) ◽  
pp. 1313-1318 ◽  
Author(s):  
Yanping Wang ◽  
Qiaoling Cheng ◽  
Zaheer Ahmed ◽  
Xiaoxue Jiang ◽  
Xiaojia Bai
Keyword(s):  
Partial Characterization ◽  
Rice Wine ◽  
Milk Clotting ◽  
Glutinous Rice ◽  
Milk Clotting Enzyme

scholarly journals Characterization of a Milk-clotting Enzyme from Hericium erinaceum and Its Proteolytic Action on Bovine Caseins

2018 ◽  
Vol 24 (4) ◽  
pp. 669-676 ◽  
Author(s):  
Kaoru Sato ◽  
Kenya Goto ◽  
Azusa Suzuki ◽  
Takayuki Miura ◽  
Motoi Endo ◽  
...  
Keyword(s):  
Milk Clotting ◽  
Milk Clotting Enzyme ◽  
Hericium Erinaceum ◽  
Proteolytic Action

Production of a Milk-Clotting Enzyme by Glutinous Rice Fermentation and Partial Characterization of the Enzyme

2015 ◽  
Vol 39 (1) ◽  
pp. 70-79 ◽  
Author(s):  
Xiao Zhao ◽  
Ji Wang ◽  
Zhe Zheng ◽  
Aimei Zhao ◽  
Zhennai Yang
Keyword(s):  
Partial Characterization ◽  
Milk Clotting ◽  
Glutinous Rice ◽  
Milk Clotting Enzyme

scholarly journals Characterization of partially purified milk‐clotting enzyme from sunflower ( Helianthus annuus ) seeds

2016 ◽  
Vol 4 (5) ◽  
pp. 733-741 ◽  
Author(s):  
Assia I. A. M. Nasr ◽  
Isam A. Mohamed Ahmed ◽  
Omer I. A. Hamid
Keyword(s):  
Helianthus Annuus ◽  
Milk Clotting ◽  
Milk Clotting Enzyme
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