A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from cicada (Cicada flammata)

2009 ◽  
Vol 86 (5) ◽  
pp. 1409-1418 ◽  
Author(s):  
Xiu Juan Ye ◽  
Tzi Bun Ng
Keyword(s):  
Reverse Transcriptase ◽  
Inhibitory Activities ◽  
Hiv 1

scholarly journals HIV-1 Protease and Reverse Transcriptase Inhibitory Activities of Curcuma aeruginosa Roxb. Rhizome Extracts and the Phytochemical Profile Analysis: In Vitro and In Silico Screening

2021 ◽  
Vol 14 (11) ◽  
pp. 1115
Author(s):  
Chanin Sillapachaiyaporn ◽  
Panthakarn Rangsinth ◽  
Sunita Nilkhet ◽  
Nuntanat Moungkote ◽  
Siriporn Chuchawankul
Keyword(s):  
Antioxidant Activity ◽  
Reverse Transcriptase ◽  
In Silico ◽  
Active Sites ◽  
Profile Analysis ◽  
Total Phenolic ◽  
Inhibitory Activities ◽  
Anti Hiv ◽  
Hiv 1

Human immunodeficiency virus type-1 (HIV-1) infection causes acquired immunodeficiency syndrome (AIDS). Currently, several anti-retroviral drugs are available, but adverse effects of these drugs have been reported. Herein, we focused on the anti-HIV-1 activity of Curcuma aeruginosa Roxb. (CA) extracted by hexane (CA-H), ethyl acetate (CA-EA), and methanol (CA-M). The in vitro HIV-1 protease (PR) and HIV-1 reverse transcriptase (RT) inhibitory activities of CA extracts were screened. CA-M potentially inhibited HIV-1 PR (82.44%) comparable to Pepstatin A (81.48%), followed by CA-EA (67.05%) and CA-H (47.6%), respectively. All extracts exhibited moderate inhibition of HIV-1 RT (64.97 to 76.93%). Besides, phytochemical constituents of CA extracts were identified by GC-MS and UPLC-HRMS. Fatty acids, amino acids, and terpenoids were the major compounds found in the extracts. Furthermore, drug-likeness parameters and the ability of CA-identified compounds on blocking of the HIV-1 PR and RT active sites were in silico investigated. Dihydroergocornine, 3β,6α,7α-trihydroxy-5β-cholan-24-oic acid, and 6β,11β,16α,17α,21-Pentahydroxypregna-1,4-diene-3,20-dione-16,17-acetonide showed strong binding affinities at the active residues of both HIV-1 PR and RT. Moreover, antioxidant activity of CA extracts was determined. CA-EA exhibited the highest antioxidant activity, which positively related to the amount of total phenolic content. This study provided beneficial data for anti-HIV-1 drug discovery from CA extracts.

A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica

2010 ◽  
Vol 27 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Shuang Zhao ◽  
Yongchang Zhao ◽  
Shuhong Li ◽  
Jingkun Zhao ◽  
Guoqing Zhang ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Wild Mushroom ◽  
Inhibitory Activities ◽  
Hiv 1 ◽  
Russula Delica

scholarly journals Antitumor and HIV-1 Reverse Transcriptase Inhibitory Activities of a Hemagglutinin and a Protease Inhibitor from Mini-Black Soybean

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Xiu Juan Ye ◽  
Tzi Bun Ng
Keyword(s):  
Breast Cancer ◽  
Reverse Transcriptase ◽  
Inhibitory Activity ◽  
Breast Cancer Cells ◽  
Deae Cellulose ◽  
Defense Proteins ◽  
Black Soybean ◽  
Trypsin Inhibitory Activity ◽  
Inhibitory Activities ◽  
Hiv 1

Protease inhibitors (PIs) and hemagglutinins are defense proteins produced by many organisms. From Chinese mini-black soybeans, a 17.5-kDa PI was isolated using chromatography on Q-Sepharose, SP-Sepharose, and DEAE-cellulose. A 25-kDa hemagglutinin was purified similarly, but using Superdex 75 instead of DEAE-cellulose in the final step. The PI inhibited trypsin and chymotrypsin (IC50= 7.2 and 8.8 μM). Its trypsin inhibitory activity was stable from pH 2 to pH 13 and from 0∘C to 70∘C. The hemagglutinin activity of the hemagglutinin was stable from pH 2 to pH 13 and from 0∘C to 75∘C. The results indicated that both PI and hemagglutinin were relatively thermostable and pH-stable. The trypsin inhibitory activity was inhibited by dithiothreitol, signifying the importance of the disulfide bond to the activity. The hemagglutinating activity was inhibited most potently by D (+)-raffinose and N-acetyl-D-galactosamine, suggesting that the hemagglutinin was specific for these two sugars. Both PI and hemagglutinin inhibited HIV-1 reverse transcriptase (IC50= 3.2 and 5.5 μM), proliferation of breast cancer cells (IC50= 9.7 and 3.5 μM), and hepatoma cells (IC50= 35 and 6.2 μM), with relatively high potencies.

scholarly journals Synthesis of Quinolin-2-one Alkaloid Derivatives and Their Inhibitory Activities against HIV-1 Reverse Transcriptase

Molecules ◽  
2011 ◽  
Vol 16 (9) ◽  
pp. 7649-7661 ◽  
Author(s):  
Pi Cheng ◽  
Qiong Gu ◽  
Wei Liu ◽  
Jian-Feng Zou ◽  
Yang-Yong Ou ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Inhibitory Activities ◽  
Hiv 1

Purification and characterization of a protein with antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities from small brown-eyed cowpea seeds

2013 ◽  
Vol 60 (4) ◽  
pp. 393-398 ◽  
Author(s):  
Guo-Ting Tian ◽  
Meng Juan Zhu ◽  
Ying Ying Wu ◽  
Qin Liu ◽  
He Xiang Wang ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Purification And Characterization ◽  
Cowpea Seeds ◽  
Inhibitory Activities ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document