Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications

2021 ◽  
Author(s):  
Radin Shafierul Radin Yahaya ◽  
Yahaya M. Normi ◽  
Lai Yee Phang ◽  
Siti Aqlima Ahmad ◽  
Janna Ong Abdullah ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Industrial Applications ◽  
Expression Systems ◽  
Heterologous Expression Systems

Kv3.1–Kv3.2 Channels Underlie a High-Voltage–Activating Component of the Delayed Rectifier K+ Current in Projecting Neurons From the Globus Pallidus

1999 ◽  
Vol 82 (3) ◽  
pp. 1512-1528 ◽  
Author(s):  
R. Hernández-Pineda ◽  
A. Chow ◽  
Y. Amarillo ◽  
H. Moreno ◽  
M. Saganich ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Globus Pallidus ◽  
High Frequency ◽  
Calcium Binding ◽  
Repetitive Firing ◽  
Delayed Rectifier ◽  
Expression Systems ◽  
Electrophysiological Properties ◽  
Channel Proteins ◽  
Heterologous Expression Systems

The globus pallidus plays central roles in the basal ganglia circuitry involved in movement control as well as in cognitive and emotional functions. There is therefore great interest in the anatomic and electrophysiological characterization of this nucleus. Most pallidal neurons are GABAergic projecting cells, a large fraction of which express the calcium binding protein parvalbumin (PV). Here we show that PV-containing pallidal neurons coexpress Kv3.1 and Kv3.2 K+ channel proteins and that both Kv3.1 and Kv3.2 antibodies coprecipitate both channel proteins from pallidal membrane extracts solubilized with nondenaturing detergents, suggesting that the two channel subunits are forming heteromeric channels. Kv3.1 and Kv3.2 channels have several unusual electrophysiological properties when expressed in heterologous expression systems and are thought to play special roles in neuronal excitability including facilitating sustained high-frequency firing in fast-spiking neurons such as interneurons in the cortex and the hippocampus. Electrophysiological analysis of freshly dissociated pallidal neurons demonstrates that these cells have a current that is nearly identical to the currents expressed by Kv3.1 and Kv3.2 proteins in heterologous expression systems, including activation at very depolarized membrane potentials (more positive than −10 mV) and very fast deactivation rates. These results suggest that the electrophysiological properties of native channels containing Kv3.1 and Kv3.2 proteins in pallidal neurons are not significantly affected by factors such as associated subunits or postranslational modifications that result in channels having different properties in heterologous expression systems and native neurons. Most neurons in the globus pallidus have been reported to fire sustained trains of action potentials at high-frequency. Kv3.1–Kv3.2 voltage-gated K+channels may play a role in helping maintain sustained high-frequency repetitive firing as they probably do in other neurons.

Heterologous Expression Systems for Studying Placental Transporters

2006 ◽  
pp. 285-300
Author(s):  
Vadivel Ganapathy ◽  
You Jun Fei ◽  
Puttur D. Prasad
Keyword(s):  
Heterologous Expression ◽  
Expression Systems ◽  
Heterologous Expression Systems

scholarly journals Identification of microalgal chloroplast sequences: genetic tools to develop microalgal heterologous expression systems for aquaculture applications

2009 ◽  
Vol 25 ◽  
pp. S40 ◽  
Author(s):  
V. Henriquez ◽  
J. Gimpel ◽  
C. Escobar ◽  
C. Gutierrez ◽  
J.P. Cadoret ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Expression Systems ◽  
Genetic Tools ◽  
Heterologous Expression Systems

scholarly journals Heterodimerization of two pore domain K+ channel TASK1 and TALK2 in living heterologous expression systems

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0186252 ◽  
Author(s):  
Yoshiaki Suzuki ◽  
Kanako Tsutsumi ◽  
Tatsuya Miyamoto ◽  
Hisao Yamamura ◽  
Yuji Imaizumi
Keyword(s):  
Heterologous Expression ◽  
K Channel ◽  
Expression Systems ◽  
Heterologous Expression Systems ◽  
Pore Domain

Regulation of Serotonin2A Receptors in Heterologous Expression Systems

2002 ◽  
Vol 63 (4) ◽  
pp. 1255-1260 ◽  
Author(s):  
Michael S. Grotewiel ◽  
Elaine Sanders-Bush
Keyword(s):  
Heterologous Expression ◽  
Expression Systems ◽  
Heterologous Expression Systems
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