Pre-S Deletion Mutants Co-Exist with Wild-Type Virus at Low Viral Replication Stage in Singapore Chronic Hepatitis B Virus Carriers

2002 ◽  
Vol 44 (2) ◽  
pp. 145-147 ◽  
Author(s):  
Wei Ning Chen ◽  
Chong Jin Oon
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Wild Type Virus ◽  
Deletion Mutants ◽  
Type Virus ◽  
Wild Type ◽  
Replication Stage ◽  
Chronic Hepatitis B Virus ◽  
B Virus

scholarly journals Presence of Replicating Virus in Recombinant Hepadnavirus Stocks Results from Recombination and Can Be Eliminated by the Use of a Packaging Cell Line

2003 ◽  
Vol 77 (5) ◽  
pp. 2873-2881 ◽  
Author(s):  
Uta Klöcker ◽  
Heike Oberwinkler ◽  
Timo Kürschner ◽  
Ulrike Protzer
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Wild Type Virus ◽  
Recombinant Hepatitis ◽  
Type Virus ◽  
Wild Type ◽  
Viral Genomes ◽  
B Virus ◽  
Viral Sequences

ABSTRACT Mutant hepatitis B viruses are useful tools to study the viral life cycle and viral pathogenesis. Furthermore, recombinant hepatitis B viruses are candidate vectors for liver-directed gene therapy. Because wild-type viruses present in recombinant or mutant virus stocks may falsify experimental results and are detrimental for a viral vector, we investigated whether and to what extent wild-type virus is present in recombinant virus stocks and where it originates from. We took advantage of the duck model of hepatitis B virus infection which allows very sensitive detection of replication-competent viruses by infection of primary duck hepatocytes or of ducklings in vivo. Recombinant hepatitis B virus stocks contained significant amounts of wild-type viruses, which were most probably generated by homologous recombination between plasmids containing homologous viral sequences. In addition, replication-competent viral genomes were reconstituted from plasmids which contained replication-deficient but redundant viral sequences. Using a stable cell line for packaging of deficient viral genomes, no wild-type virus was detected, neither by infection of primary hepatocytes nor in vivo.

A novel hepatitis B virus mutant coexisting with wild type virus in a carrier with negative HBsAg yet positive HBeAg and anti-HBs

2009 ◽  
Vol 46 (4) ◽  
pp. 363-366 ◽  
Author(s):  
Yi-Hua Zhou ◽  
Jianxin Zhou ◽  
Lei Li ◽  
Yongchun Bi ◽  
Yong Liu ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Wild Type Virus ◽  
Type Virus ◽  
Wild Type ◽  
B Virus ◽  
Virus Mutant ◽  
Positive Hbeag

scholarly journals Enrichment of a Precore-Minus Mutant of Duck Hepatitis B Virus in Experimental Mixed Infections

1999 ◽  
Vol 73 (5) ◽  
pp. 3616-3622 ◽  
Author(s):  
Yong-Yuan Zhang ◽  
Jesse Summers
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Mutant Virus ◽  
Wild Type Virus ◽  
Type Virus ◽  
Wild Type ◽  
Duck Hepatitis B Virus ◽  
Duck Hepatitis ◽  
B Virus ◽  
Precore Mutant

ABSTRACT A precore-deficient mutant of duck hepatitis B virus (DHBV) produced by site-directed mutagenesis was tested for its ability to compete with wild-type virus in a mixed infection of 3-day-old ducklings. The mutation was shown to produce a cis-acting defect, resulting in a replication rate that was about one-half that of wild-type virus. Accordingly, wild-type virus was rapidly selected during the spread of infection. During the chronic phase of the infection, however, two selection patterns were seen. In 4 of 10 ducks, the wild-type virus slowly replaced the precore mutant. In another four ducks, the precore mutant virus slowly replaced the wild-type virus. In the remaining two ducklings, ratios of wild-type and precore mutant virus fluctuated, with wild-type virus slowly predominating. The replacement of wild-type virus was not due to the emergence of a rapidly replicating variant of the precore mutant, since genomes cloned from the infected ducks retained their original replication defect. Replacement of wild-type virus, however, correlated with elevated anti-core antibody titers, which continued to increase with time. The selection of a precore-negative strain of DHBV may be analogous to the selection for precore mutants of HBV during chronic hepatitis in humans.

scholarly journals Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection

1999 ◽  
Vol 96 (19) ◽  
pp. 10818-10823 ◽  
Author(s):  
U. Protzer ◽  
M. Nassal ◽  
P.-W. Chiang ◽  
M. Kirschfink ◽  
H. Schaller
Keyword(s):  
Hepatitis B Virus ◽  
Gene Transfer ◽  
Virus Infection ◽  
Hepatitis B ◽  
Virus Vector ◽  
Wild Type Virus ◽  
Type Virus ◽  
Wild Type ◽  
B Virus ◽  
Interferon Gene
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