cAMP Receptor Protein of Salmonella enterica Serovar Typhimurium Modulate Glycolysis in Macrophages to Induce Cell Apoptosis

2018 ◽  
Vol 76 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Ke Ding ◽  
Chunjie Zhang ◽  
Jing Li ◽  
Songbiao Chen ◽  
Chengshui Liao ◽  
...  
Keyword(s):  
Cell Apoptosis ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Serovar Typhimurium ◽  
Induce Cell Apoptosis ◽  
Camp Receptor

Influence of RpoS, cAMP-receptor protein, and ppGpp on expression of the opgGH operon and osmoregulated periplasmic glucan content of Salmonella enterica serovar Typhimurium

2009 ◽  
Vol 55 (11) ◽  
pp. 1284-1293 ◽  
Author(s):  
Cristina S. Costa ◽  
Ramón A. Pizarro ◽  
Dora N. Antón
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Regulatory System ◽  
Receptor Protein ◽  
Transcriptional Level ◽  
Camp Receptor Protein ◽  
High Osmolarity ◽  
Serovar Typhimurium ◽  
Camp Receptor ◽  
Periplasmic Glucan

A transcriptional fusion (opgG1::MudJ) to the opgGH operon of Salmonella enterica serovar Typhimurium (S. Typhimurium) LT2, isolated by resistance to mecillinam, was used to study the influence of global regulators RpoS, ppGpp, and cAMP/cAMP-receptor protein (CRP) on expression of the opgGH operon and osmoregulated periplasmic glucan (OPG) content. Neither high growth medium osmolarity nor absence of ppGpp or CRP had important effects on opgG1::MudJ expression in exponential cultures. However, under the same conditions, OPG content was strongly decreased by high osmolarity or cAMP/CRP defectiveness, and reduced to a half by lack of ppGpp. In stationary cultures, high osmolarity as well as CRP loss caused significant descents in opgG1::MudJ expression that were compensated by inactivation of RpoS σ factor. No effect of RpoS inactivation on OPG content was observed. It is concluded that opgGH expression in S. Typhimurium is only slightly affected by high osmolarity, but is inversely modulated by RpoS level. On the other hand, osmolarity and the cAMP/CRP global regulatory system appear to control OPG content, either directly or indirectly, mainly at the post-transcriptional level.

Transcriptional regulation of sdiA by cAMP-receptor protein, LeuO, and environmental signals in Salmonella enterica serovar Typhimurium

2012 ◽  
Vol 58 (1) ◽  
pp. 10-22 ◽  
Author(s):  
Amy L. Turnbull ◽  
Wook Kim ◽  
Michael G. Surette
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Late Stationary Phase ◽  
Environmental Signals ◽  
Serovar Typhimurium ◽  
Elevated Expression ◽  
Transcriptional Changes ◽  
Camp Receptor

The sdiA gene encodes for a LuxR-type transcription factor, which is active when bound to N-acyl homoserine lactones (AHLs). Because Salmonella enterica serovar Typhimurium does not produce AHLs, SdiA senses signals produced by other organisms. SdiA is not expressed constitutively, and response is limited to conditions in which elevated expression occurs, but little is known about the regulation of sdiA expression. Here we map the sdiA promoter and define several regulators that directly or indirectly act on the promoter. The major activator of sdiA expression is cAMP-receptor protein (CRP), and we define the CRP operator in the sdiA promoter using promoter and crp mutants. LeuO activates sdiA expression to a lesser extent than does CRP. We demonstrate that LeuO directly binds the sdiA promoter and the Rcs phosphorelay represses sdiA expression. In this study, NhaR, IlvY, and Fur affected sdiA expression indirectly and weakly. Expression in late-stationary phase depended on RpoS. AHL-dependent expression of the SdiA-regulated gene rck correlated to the observed sdiA transcriptional changes in regulator mutants. The data demonstrate that regulation of sdiA involves integration of multiple environmental and metabolic signals.

scholarly journals cAMP receptor protein (CRP) positively regulates the yihU–yshA operon in Salmonella enterica serovar Typhi

Microbiology ◽  
2011 ◽  
Vol 157 (3) ◽  
pp. 636-647 ◽  
Author(s):  
J. M. Villarreal ◽  
I. Hernández-Lucas ◽  
F. Gil ◽  
I. L. Calderón ◽  
E. Calva ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Salmonella Enterica ◽  
Regulatory Region ◽  
Enteric Bacteria ◽  
Transcriptional Unit ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Salmonella Enterica Serovar Typhi ◽  
Genetic Components ◽  
Camp Receptor

Salmonella enterica serovar Typhi (S. Typhi) is the aetiological agent of typhoid fever in humans. This bacterium is also able to persist in its host, causing a chronic disease by colonizing the spleen, liver and gallbladder, in the last of which the pathogen forms biofilms in order to survive the bile. Several genetic components, including the yihU–yshA genes, have been suggested to be involved in the survival of Salmonella in the gallbladder. In this work we describe how the yihU–yshA gene cluster forms a transcriptional unit regulated positively by the cAMP receptor global regulator CRP (cAMP receptor protein). The results obtained show that two CRP-binding sites on the regulatory region of the yihU–yshA operon are required to promote transcriptional activation. In this work we also demonstrate that the yihU–yshA transcriptional unit is carbon catabolite-repressed in Salmonella, indicating that it forms part of the CRP regulon in enteric bacteria.

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