ABSTRACTThe main objective of this study was to decipher the molecular mechanism of resistance to carbapenems and aminoglycosides in a large series of 150Acinetobacter baumanniiclinical isolates collected from July 2012 to September 2013 in Egypt. We report for the first time the emergence ofblaNDM-1and the cooccurrence of 16S rRNA methylasearmAwithblaNDM-1andblaOXA-23in Egyptian hospitals. Multilocus sequence typing identified 27 distinct sequence types, 11 of which were novel.