Prevalence of 16S rRNA methylase genes among β-lactamase-producing Enterobacteriaceae clinical isolates in Saudi Arabia

The aim of this study was to investigate the mechanisms responsible for resistance to antimicrobials in a collection of enterobacterial isolates recovered from two hospitals in Saudi Arabia. A total of six strains isolated from different patients showing high resistance to carbapenems was recovered in 2015 from two different hospitals, with four being Klebsiella pneumoniae and two Enterobacter cloacae. All isolates except one K. pneumoniae were resistant to tigecycline, but only one K. pneumoniae was resistant to colistin. All produced a carbapenemase according to the Carba NP test, and all were positive for the EDTA-disk synergy test for detection of MBL. Using PCR followed by sequencing, the four K. pneumoniae isolates produced the carbapenemase NDM-1, while the two E. cloacae isolates produced the carbapenemase VIM-1. Genotyping analysis by Multilocus Sequence Typing (MLST) showed that three out of the four K. pneumoniae isolates were clonally related. They had been recovered from the same hospital and belonged to Sequence Type (ST) ST152. In contrast, the fourth K. pneumoniae isolate belonged to ST572. Noticeably, the NDM-1-producing K. pneumoniae additionally produced an extended-spectrum ß-lactamase (ESBL) of the CTX-M type, together with OXA-1 and TEM-1. Surprisingly, the three clonally related isolates produced different CTX-M variants, namely, CTX-M-3, CTX-M-57, and CTX-M-82, and coproduced QnrB, which confers quinolone resistance, and the 16S rRNA methylase RmtC, which confers high resistance to all aminoglycosides. The AAC(6′)-Ib acetyltransferase was detected in both K. pneumoniae and E. cloacae. Mating-out assays using Escherichia coli as recipient were successful for all isolates. The blaNDM-1 gene was always identified on a 70-kb plasmid, whereas the blaVIM-1 gene was located on either a 60-kb or a 150-kb plasmid the two E. cloacae isolates, respectively. To the best of our knowledge, this is the first report of the coexistence of an MBL (NDM-1), an ESBL (CTX-M), a 16S rRNA methylase (RmtC), an acetyltransferase (AAC[6′]-Ib), and a quinolone resistance enzyme (QnrB) in K. pneumoniae isolates recovered from different patients during an outbreak in a Saudi Arabian hospital.

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NDM-1 Metallo-β-Lactamase and ArmA 16S rRNA methylase producing Providencia rettgeri clinical isolates in Nepal

BMC Infectious Diseases ◽

10.1186/1471-2334-14-56 ◽

2014 ◽

Vol 14 (1) ◽

Cited By ~ 14

Author(s):

Tatsuya Tada ◽

Tohru Miyoshi-Akiyama ◽

Rajan K Dahal ◽

Manoj K Sah ◽

Hiroshi Ohara ◽

...

Keyword(s):

16S Rrna ◽

Clinical Isolates ◽

Providencia Rettgeri ◽

16S Rrna Methylase

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Co-production of SFO-1 and DHA-1 -lactamases and 16S rRNA methylase ArmA in clinical isolates of Klebsiella pneumoniae

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dks244 ◽

2012 ◽

Vol 67 (10) ◽

pp. 2361-2366 ◽

Cited By ~ 9

Author(s):

Q. Guo ◽

P. Wang ◽

Y. Ma ◽

Y. Yang ◽

X. Ye ◽

...

Keyword(s):

16S Rrna ◽

Klebsiella Pneumoniae ◽

Clinical Isolates ◽

16S Rrna Methylase

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Highly multidrug-resistant Morganella morganii clinical isolates from Nepal co-producing NDM-type metallo-β-lactamases and the 16S rRNA methylase ArmA

Journal of Medical Microbiology ◽

10.1099/jmm.0.001160 ◽

2020 ◽

Vol 69 (4) ◽

pp. 572-575

Author(s):

Shovita Shrestha ◽

Tatsuya Tada ◽

Jatan B. Sherchan ◽

Hiroki Uchida ◽

Tomomi Hishinuma ◽

...

Keyword(s):

16S Rrna ◽

Control Systems ◽

Type Species ◽

Multidrug Resistant ◽

Clinical Isolates ◽

Morganella Morganii ◽

Content Type ◽

Link Type ◽

16S Rrna Methylase ◽

Multiple Antibiotics

Morganella morganii can harbour extended-spectrum β-lactamases and carbapenemases, resulting in increased resistance to multiple antibiotics and a high mortality rate. This study describes the emergence of highly multidrug-resistant clinical isolates of M. morganii from Nepal co-producing NDM-type metallo-β-lactamases, including NDM-1 and NDM-5, and the 16S rRNA methylase ArmA. This is the first report of M. morganii clinical isolates from Nepal co-producing NDM-1/-5 and ArmA. It is important to establish infection control systems and effective treatments against multidrug-resistant M. morganii .

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Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01425-17 ◽

2017 ◽

Vol 61 (12) ◽

Cited By ~ 9

Author(s):

Basudha Shrestha ◽

Tatsuya Tada ◽

Kayo Shimada ◽

Shovita Shrestha ◽

Hiroshi Ohara ◽

...

Keyword(s):

Escherichia Coli ◽

16S Rrna ◽

Clinical Isolates ◽

Content Type ◽

Carbapenem Resistant ◽

Methylase Gene ◽

16S Rrna Methylase

ABSTRACT Of 250 clinical isolates of Escherichia coli obtained in Nepal, 38 were carbapenem resistant, with MICs of imipenem or meropenem of ≥4 μg/ml. All 38 isolates harbored the following bla NDMs: bla NDM-1, bla NDM-3, bla NDM-4, bla NDM-5, bla NDM-7, bla NDM-12, and bla NDM-13. Most of these isolates also harbored the 16S rRNA methylase gene(s) armA, rmtB, and/or rmtC.

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High Prevalence of 16S rRNA methylase genes High Prevalence of 16S rRNA methylase genes Among Carbapenem-resistant Hypervirulent Klebsiella pneumoniae Isolates in China

10.21203/rs.2.14808/v1 ◽

2019 ◽

Author(s):

Wenjian Liao ◽

Dan Li ◽

Dan Dan Wei ◽

Fang-lin Du ◽

Dan Long ◽

...

Keyword(s):

16S Rrna ◽

Klebsiella Pneumoniae ◽

Clinical Isolates ◽

Aminoglycoside Resistance ◽

Pfge Typing ◽

Carbapenem Resistant ◽

Carbapenemase Gene ◽

High Prevalence ◽

High Level ◽

16S Rrna Methylase

Abstract Background : the existence of 16S rRNA methylase genes would increase treatment difficulty of patients infected with CR-hvKP strains, this study was aimed to testify the prevalence of the 16S rRNA methylase genes genes in the CR-hvKP strains in China.Methods : Thirty-nine carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates collected from a Chinese hospital during the whole year of 2018 were evaluated to characterize the prevalence of 16S rRNA methylase genes. Results : In tatal 66.7% (26/39) of the CR-hvKP isolates were found to carry 16S rRNA methylase genes, and the most frequently detected gene was armA (11,42.3%), followed by rmtB (8,30.8%),and 7 CR-hvKP strains were found to carry both armA and rmtB (26.9%). All the clinical isolates were found to carry at least one carbapenemase gene,with KPC-2 (79.5%,31/39), NDM-1 (10.3%,4/39), and cocarrying KPC-2 and NDM-1 (10.3%,4/39). A total of 89.7% (35/39) isolates carried ESBL genes, including 61.5% (24/39) blaSHV-1 ,71.8% (28/39) blaTEM-1 and 89.7% (35/39) blaCTX-M-1 4. All except four isolates (89.7%,35/39) harbored PMQR genes,with qnrS (82.1%,32/39), aac(6’)-Ib-cr (79.5%,31/39), qnrB (2.6%,1/39).All the 16S rRNA methylase genes-positive CR-hvKP strains were firstly found to cocarry carbapenemase genes, ESBL genes and PMQR genes simultaneously. The most prevalent virulence genes were rmpA2 and entB (100%, 39/39),followed by silS (97.4%, 38/39), ybtS (94.9%, 37/39), iutA (92.3%, 36/39), kpn (92.3%, 36/39), rmpA (87.2%, 34/39), terW (84.6%, 33/39), aerobactin (23.1%, 9/39), repA (17.9%, 7/39), magA (10.3%, 4/39), kfuB C (10.3%, 4/39), w ca G (10.3%, 4/39), allS (10.3%, 4/39). Multilocus sequence typing (MLST) analysis assigned the 39 CR-hvKP isolates into 4 sequence types (STs), with ST11 encompassing 79.5% of the strains. Pulsed field gel electrophoresis (PFGE) typing showed that strains closely related by MLST clustered in major PFGE clusters, of which cluster A accounts for 31 ST11 isolates.The analysis of the transconjugants showed a high-level aminoglycoside resistance and a popular cotransfer of bla KPC-2 with the 16S rRNA methylase genes.Conclusions : 16S rRNA methylase genes are highly prevalent in CR-hvKP clinical isolates especially for ST11, it is therefore critical to continuously monitor the 16S rRNA methylase-producing CR-hvKP epidemiology and minimize potential risks from aminoglycoside -resistant CR-hvKP.

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