Evaluation of Antagonistic and Plant Growth Promoting Potential of Streptomyces sp. TT3 Isolated from Tea (Camellia sinensis) Rhizosphere Soil

2020 ◽  
Vol 77 (8) ◽  
pp. 1829-1838
Author(s):  
Jintu Dutta ◽  
Debajit Thakur
Keyword(s):  
Plant Growth ◽  
Camellia Sinensis ◽  
Rhizosphere Soil ◽  
Streptomyces Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Endophytic Actinomycetes from Tea Plants (Camellia sinensis): Isolation, Abundance, Antimicrobial, and Plant-Growth-Promoting Activities

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Wenna Shan ◽  
Ying Zhou ◽  
Huihui Liu ◽  
Xiaomin Yu
Keyword(s):  
Plant Growth ◽  
Camellia Sinensis ◽  
Bioactive Metabolites ◽  
Endophytic Actinomycetes ◽  
16S Rna ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Abundance And Diversity ◽  
Tea Plants ◽  
Promising Source

Endophytic actinomycetes are a promising source of novel metabolites with diverse biological activities. Tea plants (Camellia sinensis) produce arsenals of phytochemicals, which are linked to a number of medicinal and nutritional properties. However, a systematic investigation into the abundance and diversity of cultivated actinomycetes residing in tea plants has not been performed. In this study, a total of 46 actinobacteria were recovered from leaf, stem, and root samples of 15 tea cultivars collected in Fujian province, China. Their abundance and diversity were shown to be influenced by both the genotypes and tissue types of tea plants. Based on 16S RNA sequence analysis, these isolates were taxonomically grouped into 11 families and 13 genera, includingStreptomyces,Actinomadura,Kribbella,Nocardia,Kytococcus,Leifsonia,Microbacterium,Micromonospora,Mobilicoccus,Mycobacterium,Nocardiopsis,Piscicoccus, andPseudonocardia. The genusStreptomyceswas most prevalent whereas rare genera,MobilicoccusandPiscicoccus, were reported for the first time to occur as plant endophytes. PCR screening of polyketide synthase genes (PKS-I and PKS-II) and nonribosomal peptide synthetase genes (NRPS), along with antimicrobial assays against a set of bacterial and fungal pathogens, showed that endophytic actinomycetes associated with tea plants have a high potential for producing antimicrobial metabolites. Furthermore, indole acetic acid (IAA) production and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activities were recorded in 93.5% and 21.7% of all isolates, respectively. Overall, these results indicate that endophytic actinomycetes from tea plants represent a valuable source of bioactive metabolites with antibacterial, antifungal, and plant-growth-promoting properties.

scholarly journals Arachidicoccus rhizosphaerae gen. nov., sp. nov., a plant-growth-promoting bacterium in the family Chitinophagaceae isolated from rhizosphere soil

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 578-586 ◽  
Author(s):  
Munusamy Madhaiyan ◽  
Selvaraj Poonguzhali ◽  
Murugaiyan Senthilkumar ◽  
Dhandapani Pragatheswari ◽  
Jung-Sook Lee ◽  
...  
Keyword(s):  
Plant Growth ◽  
Type Strain ◽  
Type Species ◽  
Rhizosphere Soil ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Plant Growth Promoting ◽  
Growth Promoting

Three novel bacterial strains, designated Vu-144T, Vu-7 and Vu-35, were isolated on minimal medium from rhizosphere soil of field-grown cowpea and subjected to a taxonomic study using a polyphasic approach. Cells of the strains were Gram-stain-negative, non-motile, non-spore-forming, coccoid rods, and formed non-pigmented colonies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Vu-144T was affiliated with an uncultivated lineage of the phylum Bacteroidetes . Its closest phylogenetic neighbour was the recently described species Niastella populi , a member of the family Chitinophagaceae , with just 90.7 % sequence similarity to the type strain. The only isoprenoid quinone detected was menaquinone 7 (MK-7). The fatty acid profiles showed large amounts of iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G and minor amounts of summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and other fatty acids, allowing the differentiation of the strains from other genera. The G+C content of the genomic DNA of the three strains ranged from 43.1 to 44.3 mol%. In addition to phosphatidylethanolamine, the major polar lipids were three unidentified aminophospholipids (APL1–APL3), two unidentified phospholipids (PL1, PL2) and three unidentified lipids (UL1–UL3). Biochemical test patterns also differed from those of Niastella populi and members of other genera. All three isolates showed plant-growth-promoting properties, e.g. the ability to produce indole-3-acetic acid and NH3 and to solubilize phosphate, utilized 1-aminocyclopropane 1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions. Inoculated plants showed a 45–60 % increase in dry matter weight with respect to uninoculated controls. On the basis of the evidence from our polyphasic study, isolate Vu-144T represents a novel genus and species in the family Chitinophagaceae , for which the name Arachidicoccus rhizosphaerae gen. nov., sp. nov. is proposed. The type strain of Arachidicoccus rhizosphaerae is Vu-144T ( = KCTC 22378T = NCIMB 14473T).

scholarly journals Deciphering Rhizosphere Soil System for Strains Having Plant Growth Promoting and Bioremediation Traits

2012 ◽  
Vol 1 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Keshav Prasad Shukla ◽  
Shivesh Sharma ◽  
Nand Kumar Singh ◽  
Vasudha Singh
Keyword(s):  
Plant Growth ◽  
Rhizosphere Soil ◽  
Soil System ◽  
Plant Growth Promoting ◽  
Growth Promoting

Evaluation of rhizosphere Streptomyces for zinc solubilizing and plant growth promoting properties

2021 ◽  
pp. 36-47
Author(s):  
K. Balakrishnan ◽  
Manikkam Radhakrishnan ◽  
Venugopal Gopikrishnan ◽  
J. Thirumalairaj ◽  
Ramasamy Balagurunathan
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Antagonistic Activity ◽  
Cow Dung ◽  
Good Growth ◽  
Acetic Acid Production ◽  
Streptomyces Sp ◽  
Moderate Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

Actinobacteria were isolated from rhizosphere soil and its plant growth-promoting ability was tested. Actinobacteria were screened for promoting plant growth by phosphate and zinc solubilization, gibberellin, siderophore, HCN and indole-3-acetic acid production. The abiotic stresses of all the isolates were tested for parameters such as pH, temperature and NaCl. Upon screening, a potent actinobacteria named Streptomyces sp. ZS-18 was isolated and identified based on the colony characteristics. The potent strain solubilized both the zinc oxide (26 ± 0.75mm) and zinc carbonate (22 ± 0.88mm). The utilization of gibberellic acid (80μg/ml) by the potential strain ZS-18 showed maximum production measured by optical density value of 0.179. The growth of the green gram plant was determined by shoot, root, total length, number of leaves and chlorophyll estimation of the leaves. Streptomyces sp. ZS-18 showed good growth on ISP 1, ISP 2, ISP 3, ISP 5 and ISP 7 medium and moderate growth was observed in ISP 4 and ISP 6 medium. In addition, Actinobacteria were tested for antagonistic activity against plant pathogens. The isolate ZS-18 in the pot trial experiment showed increased growth in comparison with control and cow dung manure.

scholarly journals Screening and Whole-Genome Sequencing of Two Streptomyces Species from the Rhizosphere Soil of Peony Reveal Their Characteristics as Plant Growth-Promoting Rhizobacteria

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Chengqiang Wang ◽  
Yun Wang ◽  
Jinjin Ma ◽  
Qihui Hou ◽  
Kai Liu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rhizosphere Soil ◽  
Gc Content ◽  
Plant Growth Promoting Rhizobacteria ◽  
Biological Characteristics ◽  
Protein Coding ◽  
Streptomyces Species ◽  
Protein Coding Genes ◽  
Plant Growth Promoting ◽  
Growth Promoting

Two bacteria, Streptomyces albireticuli MDJK11 and S. alboflavus MDJK44, which are potential plant growth-promoting rhizobacteria against pathogenic fungi were isolated from the rhizosphere soil of peony in Shandong, China. Their biological characteristics and complete genome sequences were reported in this study. The total genome size of MDJK11 was only 8.14 Mb with 6,550 protein-coding genes and a high GC content of 72.8 mol%. The MDJK44 genome comprises a 9.62 Mb chromosome with 72.1 mol% GC content, 7,285 protein-coding genes, and two plasmids. Some gene sequences in these two genomes were analyzed to be heterologously obtained by horizontal transfer. Gene or gene cluster candidates responding to secondary metabolites production, antimicrobial activities, and plant growth-promoting capacities were also analyzed in this paper. The genomic information and biological characteristics will facilitate the understanding and application of S. albireticuli and S. alboflavus species as biocontrol agents in future agriculture.

Succinic and lactic acids as plant growth promoting compounds produced by rhizospheric Pseudomonas putida

1993 ◽  
Vol 39 (12) ◽  
pp. 1150-1154 ◽  
Author(s):  
Masami Yoshikawa ◽  
Nobuhiro Hirai ◽  
Kohji Wakabayashi ◽  
Haruyuki Sugizaki ◽  
Hajime Iwamura
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Culture Filtrate ◽  
Rhizosphere Soil ◽  
Growth Promotion ◽  
Crown Rot ◽  
Asparagus Officinalis ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Lactic Acids

In greenhouse trials, root growth of Asparagus officinalis L. increased up to 30% when roots of 3-week-old seedlings were dipped in the culture filtrate of Pseudomonas putida RSA9, a strain isolated from rhizosphere soil of asparagus and antagonistic to the crown rot pathogen Fusarium moniliforme. The culture filtrate was extracted with ethyl acetate at pH 3, and the extracts were fractionated on a column of octadesylsilica gel. The active fraction was found to be a 45:55 mixture of succinic and lactic acids. Root mass increased 40% when the roots of the seedlings were treated with a 1:1 mixture of the acids at 10 ppm. The results provide an explanation for the plant growth promoting effects of some rhizobacteria; the bacteria may secrete organic acids, such as succinic and lactic acids, and these acids may increase plant growth under conditions in which the populations of pathogens are reduced.Key words: succinic acid, lactic acid, Pseudomonas putida, plant growth promotion, rhizobacteria, PGPR.

scholarly journals Draft Genome Sequence of Endophytic Herbaspirillum sp. Strain WT00C, a Tea Plant Growth-Promoting Bacterium

2017 ◽  
Vol 5 (11) ◽  
Author(s):  
Wei Cheng ◽  
Guiting Zhan ◽  
Weilin Liu ◽  
Rong Zhu ◽  
Xuejing Yu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Camellia Sinensis ◽  
Draft Genome ◽  
Tea Plant ◽  
Draft Genome Sequence ◽  
Elemental Selenium ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Endophytic Herbaspirillum sp. strain WT00C was isolated from tea plant (Camellia sinensis L.). Here, we report the 6.08 Mb draft genome sequence of this strain, providing bioinformation about its agronomic benefits and capability to reduce selenate/selenite into red elemental selenium.

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