Cell-division-cycle defects associated with fission yeast pre-mRNA splicing mutants

1998 ◽  
Vol 34 (3) ◽  
pp. 153-163 ◽  
Author(s):  
Judith Potashkin ◽  
Daemyung Kim ◽  
Mark Fons ◽  
Tim Humphrey ◽  
David Frendewey
Keyword(s):  
Cell Division ◽  
Fission Yeast ◽  
Cell Division Cycle ◽  
Mrna Splicing

scholarly journals Mathematical model of the cell division cycle of fission yeast

2001 ◽  
Vol 11 (1) ◽  
pp. 277 ◽  
Author(s):  
Bela Novak ◽  
Zsuzsa Pataki ◽  
Andrea Ciliberto ◽  
John J. Tyson
Keyword(s):  
Mathematical Model ◽  
Cell Division ◽  
Fission Yeast ◽  
Cell Division Cycle

scholarly journals A conserved cell growth cycle can account for the environmental stress responses of divergent eukaryotes

2012 ◽  
Vol 23 (10) ◽  
pp. 1986-1997 ◽  
Author(s):  
Nikolai Slavov ◽  
Edoardo M. Airoldi ◽  
Alexander van Oudenaarden ◽  
David Botstein
Keyword(s):  
Gene Expression ◽  
Growth Rate ◽  
Oxygen Consumption ◽  
Heat Stress ◽  
Cell Division ◽  
Environmental Stress ◽  
Fission Yeast ◽  
Budding Yeast ◽  
Cell Division Cycle ◽  
Metabolic Cycle

The respiratory metabolic cycle in budding yeast (Saccharomyces cerevisiae) consists of two phases that are most simply defined phenomenologically: low oxygen consumption (LOC) and high oxygen consumption (HOC). Each phase is associated with the periodic expression of thousands of genes, producing oscillating patterns of gene expression found in synchronized cultures and in single cells of slowly growing unsynchronized cultures. Systematic variation in the durations of the HOC and LOC phases can account quantitatively for well-studied transcriptional responses to growth rate differences. Here we show that a similar mechanism—transitions from the HOC phase to the LOC phase—can account for much of the common environmental stress response (ESR) and for the cross-protection by a preliminary heat stress (or slow growth rate) to subsequent lethal heat stress. Similar to the budding yeast metabolic cycle, we suggest that a metabolic cycle, coupled in a similar way to the ESR, in the distantly related fission yeast, Schizosaccharomyces pombe, and in humans can explain gene expression and respiratory patterns observed in these eukaryotes. Although metabolic cycling is associated with the G0/G1 phase of the cell division cycle of slowly growing budding yeast, transcriptional cycling was detected in the G2 phase of the division cycle in fission yeast, consistent with the idea that respiratory metabolic cycling occurs during the phases of the cell division cycle associated with mass accumulation in these divergent eukaryotes.

Distribution of tubulin and actin through the cell division cycle of the fission yeast Schizosaccharomyces japonicus var. versatilis: a comparison with Schizosaccharomyces pombe

1990 ◽  
Vol 96 (1) ◽  
pp. 71-77
Author(s):  
C.E. Alfa ◽  
J.S. Hyams
Keyword(s):  
Cell Division ◽  
Fission Yeast ◽  
Cell Biology ◽  
Cell Division Cycle ◽  
Microtubule Organization ◽  
Actin Ring ◽  
Western Blots ◽  
Cell Extracts ◽  
Tightly Coupled ◽  
Basic Studies

Changes in the distribution of microtubules and F-actin through the cell division cycle of the fission yeast Schizosaccharomyces japonicus var. versatilis were investigated by fluorescence microscopy. The fluorescence images obtained with S. japonicus were markedly superior to those previously reported for S. pombe and revealed new details of cytoskeletal organization in this important genus. As in S. pombe, F-actin in S. japonicus was present as a concentration of ‘dots’ at the growing poles of interphase cells and as a filamentous equatorial ring directing the deposition of the cytokinetic septum. The transition between these two states occurred at late anaphase, in contrast to the situation in S. pombe where the appearance of the equatorial actin ring is tightly coupled to the early events of mitosis. During the course of cytokinesis in S. japonicus the actin ring constricted and broadened, suggesting that it is contractile. Microtubule organization in S. japonicus also revealed interesting differences from S. pombe. Whereas in S. pombe cytoplasmic microtubules are reinitiated from a pair of microtubule organizing centres (MTOCs) at the cell equator, in S. japonicus they arise by extensive microtubule growth from the spindle poles. Western blots of cell extracts enriched for tubulin by DEAE-Sephadex chromatography showed that, like S. pombe, S. japonicus contains two alpha-tubulins and a single beta-tubulin. Whilst the alpha 1- and beta-tubulins from the two species comigrated on one-dimensional polyacrylamide gels, the alpha 2 species were electrophoretically distinct. Although fundamental differences clearly exist between the two species, S. japonicus could prove to be a useful tool in basic studies of fission yeast cell biology.

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