The Beer Phenolic Profile Change During «Dry» Hopping in the Post-fermentation Stage Conditions

Статья посвящена вопросу изменения фенольного профиля пива в условиях «холодного» охмеления во время дображивания пива. Оценено влияние метаболизма дрожжевых клеток и типа хмеля во время дображивания на изменение общего количества полифенолов, изоксантогумола, изогумулона, кверцетина и рутина. Показано, что применение низовой расы дрожжей Rh и горького типа хмеля Mагнум позволяет добиться высокого (до 123,0 мг/дм3) содержания полифенолов в пиве к 1 сут «холодного» охмеления в отличие от тонко-ароматного хмеля Tетнангера, применение которого дает максимум количества полифенолов к 14 сут процесса. Применение верховой расы дрожжей Nottinghem приводит к замедлению увеличения концентрации полифенолов в среде, поскольку только к 7-14 сут вне зависимости от типа хмеля происходит их накопление. Показана зависимость в течение первых 14 сут изменения количества изоксантогумола от расы дрожжей и типа хмеля, а в последующем - только от типа хмеля. Авторами получены результаты, свидетельствующие о том, что кверцетин не вовлекается низовыми дрожжами в метаболический цикл, в отличие от верховых. Изменение концентрации рутина в пиве не зависит от расы дрожжей, и определяется своим содержанием в хмеле определенного типа. В работе показана взаимосвязь между процессами изменения содержания изогумулона и изоксантогумола при «холодном» охмелении в зависимости от ряда факторов. Проведение органолептического анализа позволило соотнести балловую оценку дескрипторов пива с основными показателями фенольного профиля. The article is devoted to the issue of beer phenolic profile changing in the conditions of «dry» hopping during the after-fermentation of beer. The influence of the yeast cells metabolism and the hop type during fermentation on the change in the polyphenol, isoxanthohumol, isogumulone, quercetin and rutin total amount, was evaluated. It has been shown that the Rh lager yeast race use and the Magnum bitter hop type makes it possible to achieve a high (up to 123.0 mg/dm3) polyphenol content in beer by 1 day of «dry» hopping, in contrast to the finely aromatic Tettnanger hops, the use of which gives the maximum polyphenol amount by 14 days of the process. The Nottinghem ale yeast race use leads to a slowdown in the polyphenol concentration increase in the medium, since their accumulation occurs only by 7-14 days, regardless of the hop type. The dependence during the first 14 days of the change in the isoxanthohumol amount on the yeast race and the hop type, and subsequently - only on the hop type was shown. The authors obtained results indicating that quercetin is not involved in the metabolic cycle by lager yeast, in contrast to ale yeast. The change in the rutin concentration in beer does not depend on the yeast race and is determined by its content in a certain type of hop. The paper shows the relationship between the processes of changing the isogumulone and isoxanthohumol content during «dry» hopping, depending on several factors. Organoleptic analysis made it possible to correlate the beer descriptors scoring with the phenolic profile main indicators.

An endocrine-hepato-muscular metabolic cycle links skeletal muscle atrophy and hyperglycemia in type 2 diabetes

Both obesity and sarcopenia are frequently associated in ageing, and together may promote the progression of related conditions such as diabetes and frailty. However, little is known about the pathophysiological mechanisms underpinning this association. Here we uncover dysregulated systemic alanine metabolism and hyper-expression of the alanine transaminases (ALT) in the liver of obese/diabetic mice and humans. Hepatocyte-selective silencing of both ALT enzymes revealed a clear role in systemic alanine clearance which related to glycemic control. In obese/diabetic mice, not only did silencing both ALT enzymes retard hyperglycemia, but also reversed skeletal muscle atrophy. This was due to a rescue of depressed skeletal muscle protein synthesis, with a liver-skeletal muscle amino acid metabolic crosstalk exemplified by ex vivo experiments. Mechanistically, chronic liver glucocorticoid and glucagon signaling driven liver alanine catabolism promoted hyperglycemia and skeletal muscle wasting. Taken together, here we reveal an endocrine-hepato-muscular metabolic cycle linking hyperglycemia and skeletal muscle atrophy in type 2 diabetes.

GLUTATHIONE, MITOCHONDRIAL DEFECTS, AND A UNIQUE METABOLIC CYCLE IN OLDER HUMANS: IMPLICATIONS FOR SARCOPENIA

Sarcopenia in aging leads to decreased muscle mass and physical-function (muscle strength and exercise capacity), but underlying mechanisms are not well understood and effective interventions are limited. We hypothesized that deficiency of the intracellular antioxidant protein Glutathione initiates a unique self-perpetuating metabolic cycle linking impaired fasted mitochondrial fuel-oxidation (fMFO) to protein catabolism and contributes to sarcopenia. We also hypothesized that supplementing the Glutathione precursor amino-acids glycine and N-acetylcysteine (GlyNAC) to correct Glutathione deficiency in older humans could reverse these defects. We tested our hypothesis in a 24-week open-label clinical-trial in 8 older-humans (74y) studied before and 24-weeks after GlyNAC supplementation, compared to 8 gender-matched unsupplemented young-controls (25y), and measured intracellular Glutathione concentrations, fMFO, physical-function, muscle-protein breakdown-rate (MPBR), gluconeogenesis, and urine nitrogen-excretion (UNE). GlyNAC supplementation in older humans corrected Glutathione deficiency and restored impaired fMFO (to levels in young controls), lowered MPBR and UNE, and increased physical-function, but did not affect gluconeogenesis or increase lean-mass, and suggest that muscle amino-acids are utilized for energy needs rather than glucose production. The absence of an increase in lean-mass suggests that GlyNAC should be combined with anabolic agents for potential benefits in combating sarcopenia. Overall, these results indicate the presence of a unique reversible metabolic cycle in older humans initiated by Glutathione deficiency which results in impaired mitochondrial fatty-acid and glucose oxidation, muscle-protein breakdown, UNE, and leads to deficiency of glycine and cysteine which re-initiate the cycle. These data have implications for improving physical-function and muscle mass in age-associated sarcopenia, and warrants further investigation.

Circulating glutamine and Alzheimer’s disease: a Mendelian randomization study

ABSTRACTINTRODUCTIONAlzheimer’s disease is a devastating neurodegenerative disorder. Its worldwide prevalence is over 24 million and is expected to double by 2040. Finding ways to prevent its cognitive decline is urgent.METHODSA two-sample Mendelian randomization study was performed instrumenting glutamine, which is abundant in blood, capable of crossing the blood-brain barrier, and involved in a metabolic cycle with glutamate in the brain.RESULTSThe results reveal a protective effect of circulating glutamine (inverse-variance weighted method, odds ratio per 1-SD increase in circulating glutamine = 0.83; 95% CI 0.71, 0.97; P = 0.02).CONCLUSIONThese findings lend credence to the emerging story supporting the modifiability of glutamine/glutamate metabolism for the prevention of cognitive decline. More circulating glutamine might mean that more substrate is available during times of stress, acting as a neuroprotectant. Modifications to exogenous glutamine may be worth exploring in future efforts to prevent and/or treat Alzheimer’s disease.

Managing the Water-Energy Nexus within a Climate Change Context—Lessons from the Experience of Cuenca, Ecuador

The impact of climate change dynamics has a multiplicative effect when the interlinkages between water and energy are considered. This also applies to climate change co-benefits that derive from adaptation and mitigation initiatives implemented at the urban level and that address the water-energy nexus. A better understanding of the water-energy nexus is a precondition for integrated resource planning that optimizes the use of scarce resources. Against this background, the paper assesses the potential impact of water-energy saving technologies (WEST) on the water-energy nexus of Cuenca, Ecuador, focusing on how vulnerability to climate change may affect the water metabolic cycle of the urban area. Water-energy saving technologies such as rainwater harvesting, solar water heaters, and micro water turbines, reduce water-related energy consumption and mitigate greenhouse gases emissions; thereby illustrating the potential to generate climate change mitigation and adaptation co-benefits. The paper relies on primary data collected through interviews and a survey as well as secondary data in order to assess the extent to which water-energy saving technologies influence the water-energy nexus in Cuenca’s urban water metabolic cycle. Within the context of climate change, the paper develops a business-as-usual scenario and assesses how this is modified by the implementation of water-energy saving technologies.