Inhibitory effect of bone resorption and inflammation with etidronate therapy in patients with rheumatoid arthritis for 3 years and in vitro assay in arthritis models

2005 ◽  
Vol 26 (7) ◽  
pp. 627-632 ◽  
Author(s):  
Kaname Yamamoto ◽  
Shinichi Yoshino ◽  
Goukei Shue ◽  
Masakazu Nagashima
Keyword(s):  
Rheumatoid Arthritis ◽  
Bone Resorption ◽  
Inhibitory Effect ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Arthritis Models

Study on the In Vitro Assay Method for Evaluating the Inhibitory Effect of Various Substances on the Production of Plasma Kallikrein

1989 ◽  
pp. 249-253 ◽  
Author(s):  
Katsumi Nishikawa ◽  
Hitoshi Kawakubo ◽  
Kenji Matsumoto ◽  
Hisashi Yago ◽  
Yoshio Toyomaki ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
In Vitro Assay ◽  
Assay Method ◽  
Plasma Kallikrein ◽  
Vitro Assay

scholarly journals Heptad Repeat-Derived Peptides Block Protease-Mediated Direct Entry from the Cell Surface of Severe Acute Respiratory Syndrome Coronavirus but Not Entry via the Endosomal Pathway

2007 ◽  
Vol 82 (1) ◽  
pp. 588-592 ◽  
Author(s):  
Makoto Ujike ◽  
Hiroki Nishikawa ◽  
Akira Otaka ◽  
Naoki Yamamoto ◽  
Norio Yamamoto ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Cell Surface ◽  
Inhibitory Effect ◽  
In Vitro Assay ◽  
Heptad Repeat ◽  
Vitro Assay ◽  
Strong Inhibitory Effect ◽  
Endosomal Pathway ◽  
Heptad Repeats

ABSTRACT The peptides derived from the heptad repeat (HRP) of severe acute respiratory syndrome coronavirus (SCoV) spike protein (sHRPs) are known to inhibit SCoV infection, yet their efficacies are fairly low. Recently our research showed that some proteases facilitated SCoV's direct entry from the cell surface, resulting in a more efficient infection than the previously known infection via endosomal entry. To compare the inhibitory effect of the sHRP in each pathway, we selected two sHRPs, which showed a strong inhibitory effect on the interaction of two heptad repeats in a rapid and virus-free in vitro assay system. We found that they efficiently inhibited SCoV infection of the protease-mediated cell surface pathway but had little effect on the endosomal pathway. This finding suggests that sHRPs may effectively prevent infection in the lungs, where SCoV infection could be enhanced by proteases produced in this organ. This is the first observation that HRP exhibits different effects on virus that takes the endosomal pathway and virus that enters directly from the cell surface.

Inhibitory effect of iridoids on Epstein-Barr virus activation by a short-term in vitro assay for anti-tumor promoters

1996 ◽  
Vol 102 (1-2) ◽  
pp. 223-226 ◽  
Author(s):  
Govind J. Kapadia ◽  
Shekhar C. Sharma ◽  
Harukuni Tokuda ◽  
Hoyoku Nishino ◽  
Shinichi Ueda
Keyword(s):  
Epstein Barr Virus ◽  
Inhibitory Effect ◽  
In Vitro Assay ◽  
Tumor Promoters ◽  
Short Term ◽  
Vitro Assay ◽  
Barr Virus ◽  
Epstein Barr

Establishment of a rapid bone resorption in vitro assay using previiously frozen mouse unfractionated bone cells pretreated with parathyroid hormone

Bone ◽  
1993 ◽  
Vol 14 (6) ◽  
pp. 829-834 ◽  
Author(s):  
K. Kitamura ◽  
M. Katoh ◽  
O. Komiyama ◽  
H. Kitagawa ◽  
F. Matsubara ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Bone Cells ◽  
In Vitro Assay ◽  
Vitro Assay

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

A comparative study on adhesion and recovery of potential probiotic strains ofLactobacillusspp. by in vitro assay and analysis of human colon biopsies

2009 ◽  
Vol 21 (2) ◽  
Author(s):  
Nadja Larsen ◽  
Kim F. Michaelsen ◽  
Anders Pærregaard ◽  
Finn K. Vogensen ◽  
Mogens Jakobsen
Keyword(s):  
Comparative Study ◽  
Human Colon ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Probiotic Strains

Angiogenic Effect of Pravastatin Alone and with Sera from Healthy and Complicated Pregnancies Studied by in vitro Vasculogenesis/Angiogenesis Assay

2021 ◽  
pp. 1-9
Author(s):  
Anita Virtanen ◽  
Outi Huttala ◽  
Kati Tihtonen ◽  
Tarja Toimela ◽  
Tuula Heinonen ◽  
...  
Keyword(s):  
Direct Effect ◽  
Late Onset ◽  
Maternal Serum ◽  
In Vitro Assay ◽  
Serum Samples ◽  
Therapeutic Concentration ◽  
Tubule Formation ◽  
Vitro Assay ◽  
Angiogenesis Assay

<b><i>Objective:</i></b> To determine the direct effect of pravastatin on angiogenesis and to study the interaction between pravastatin and maternal sera from women with early- or late-onset pre-eclampsia (PE), intrauterine growth restriction, or healthy pregnancy. <b><i>Methods:</i></b> We collected 5 maternal serum samples from each group. The effect of pravastatin on angiogenesis was assessed with and without maternal sera by quantifying tubule formation in a human-based in vitro assay. Pravastatin was added at 20, 1,000, and 8,000 ng/mL concentrations. Concentrations of angiogenic and inflammatory biomarkers in serum and in test medium after supplementation of serum alone and with pravastatin (1,000 ng/mL) were measured. <b><i>Results:</i></b> Therapeutic concentration of pravastatin (20 ng/mL) did not have significant direct effect on angiogenesis, but the highest concentrations inhibited angiogenesis. Pravastatin did not change the levels of biomarkers in the test media. There were no changes in angiogenesis when therapeutic dose of pravastatin was added with maternal sera, but there was a trend to wide individual variation towards enhanced angiogenesis, particularly in the early-onset PE group. <b><i>Conclusions:</i></b> At therapeutic concentration, pravastatin alone or with maternal sera has no significant effect on angiogenesis, but at high concentrations the effect seems to be anti-angiogenic estimated by in vitro assay.

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