Study on the In Vitro Assay Method for Evaluating the Inhibitory Effect of Various Substances on the Production of Plasma Kallikrein

1989 ◽  
pp. 249-253 ◽  
Author(s):  
Katsumi Nishikawa ◽  
Hitoshi Kawakubo ◽  
Kenji Matsumoto ◽  
Hisashi Yago ◽  
Yoshio Toyomaki ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
In Vitro Assay ◽  
Assay Method ◽  
Plasma Kallikrein ◽  
Vitro Assay

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (2) ◽  
pp. 209-216 ◽  
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (1) ◽  
pp. 209-216
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits

2014 ◽  
Vol 153 ◽  
pp. 130-133 ◽  
Author(s):  
Gema Flores ◽  
Fernando De la Peña Moreno ◽  
Gracia Patricia Blanch ◽  
Maria Luisa Ruiz del Castillo
Keyword(s):  
Enzyme Activity ◽  
Phenylalanine Ammonia Lyase ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Flavonol Synthase ◽  
Berry Fruits

scholarly journals Heptad Repeat-Derived Peptides Block Protease-Mediated Direct Entry from the Cell Surface of Severe Acute Respiratory Syndrome Coronavirus but Not Entry via the Endosomal Pathway

2007 ◽  
Vol 82 (1) ◽  
pp. 588-592 ◽  
Author(s):  
Makoto Ujike ◽  
Hiroki Nishikawa ◽  
Akira Otaka ◽  
Naoki Yamamoto ◽  
Norio Yamamoto ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Cell Surface ◽  
Inhibitory Effect ◽  
In Vitro Assay ◽  
Heptad Repeat ◽  
Vitro Assay ◽  
Strong Inhibitory Effect ◽  
Endosomal Pathway ◽  
Heptad Repeats

ABSTRACT The peptides derived from the heptad repeat (HRP) of severe acute respiratory syndrome coronavirus (SCoV) spike protein (sHRPs) are known to inhibit SCoV infection, yet their efficacies are fairly low. Recently our research showed that some proteases facilitated SCoV's direct entry from the cell surface, resulting in a more efficient infection than the previously known infection via endosomal entry. To compare the inhibitory effect of the sHRP in each pathway, we selected two sHRPs, which showed a strong inhibitory effect on the interaction of two heptad repeats in a rapid and virus-free in vitro assay system. We found that they efficiently inhibited SCoV infection of the protease-mediated cell surface pathway but had little effect on the endosomal pathway. This finding suggests that sHRPs may effectively prevent infection in the lungs, where SCoV infection could be enhanced by proteases produced in this organ. This is the first observation that HRP exhibits different effects on virus that takes the endosomal pathway and virus that enters directly from the cell surface.

Development of a Highly Sensitive In Vitro Assay Method for Biological Activity of Endotoxin Contamination in Biological Products

Biologicals ◽  
2002 ◽  
Vol 30 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Akihiko Yamamoto ◽  
Masaki Ochiai ◽  
Michiyo Kataoka ◽  
Hiromi Toyoizumi ◽  
Yoshinobu Horiuchi
Keyword(s):  
Biological Activity ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Biological Products ◽  
Highly Sensitive ◽  
Endotoxin Contamination

EFFECTS OF INCUBATION CONDITIONS ON THE IN VITRO ASSAY METHOD FOR INSULIN

1961 ◽  
Vol 39 (9) ◽  
pp. 1381-1387 ◽  
Author(s):  
D. C. Jessup ◽  
G. S. Wiberg
Keyword(s):  
Adverse Effect ◽  
Incubation Period ◽  
Incubation Time ◽  
Glucose Concentration ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Number Of Factors

A number of factors such as incubation time, glucose concentration, and preincubation cooling affect the deposition of glycogen and the uptake of glucose by diaphragm sections in response to insulin. The greatest response occurs during the first 30 minutes of incubation but for assay purposes an incubation period of 90 minutes was recommended as it gave the greatest difference between control and treated tissues. A glucose concentration of 0.2% in the medium was found optimum. The effect of insulin at higher and lower concentrations was not as great. Preincubation cooling had an adverse effect on the response of the tissues to insulin.

THE BIOASSAY OF LONG-ACTING THYROID STIMULATOR: A COMPARISON OF TWO METHODS

1971 ◽  
Vol 49 (3) ◽  
pp. 487-492 ◽  
Author(s):  
JANICE M. ENSOR ◽  
PAT KENDALL-TAYLOR ◽  
D. S. MUNRO ◽  
B. R. SMITH
Keyword(s):  
Thyroid Stimulating Hormone ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
In Vitro Method ◽  
Long Acting ◽  
Stimulating Hormone

SUMMARY The McKenzie (1958) assay method and an in-vitro assay method (Brown & Munro, 1967) for the determination of long-acting thyroid stimulator have been compared. Differences in the results were not great: the in-vitro method is simpler to perform and more precise. The sensitivity of the two methods is similar. The in-vitro method, when used with antiserum to thyroid-stimulating hormone in the medium, provided a satisfactory and simple means for the assay of long-acting thyroid stimulator in the serum of thyrotoxic patients.

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