Evaluation of microvascular permeability of skeletal muscle and texture analysis based on DCE-MRI in alloxan-induced diabetic rabbits

2021 ◽  
Author(s):  
Baiyu Liu ◽  
Lei Hu ◽  
Li Wang ◽  
Dong Xing ◽  
Lin Peng ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Texture Analysis ◽  
Microvascular Permeability ◽  
Dce Mri ◽  
Diabetic Rabbits

scholarly journals Evaluation of Bone Marrow Texture and Trabecular Changes With Quantitative DCE-MRI and QCT in Alloxan-Induced Diabetic Rabbit Models

2021 ◽  
Vol 12 ◽  
Author(s):  
Pianpian Chen ◽  
Yunfei Zha ◽  
Li Wang ◽  
Liang Li ◽  
Lei Hu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Texture Analysis ◽  
Pearson Correlation ◽  
Quantitative Computed Tomography ◽  
Lumbar Region ◽  
Mineral Density ◽  
Dce Mri ◽  
Diabetic Rabbit ◽  
Permeability Parameter ◽  
Diabetic Rabbits

PurposeTo investigate whether the microvascular permeability of lumbar marrow and bone trabecular changes in early-stage diabetic rabbits can be quantitatively evaluated using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), quantitative computed tomography, and texture-analyzed permeability parameter Ktrans map of DCE-MRI.Materials and MethodsThis prospective study included 24 rabbits that were randomly assigned to diabetic (n = 14) and control (n = 10) groups. All rabbits underwent sagittal MRI of the lumbar region at 0, 4, 8, 12, and 16 weeks after alloxan injection. Pearson correlation coefficient was performed to determine the correlation between permeability parameter and bone mineral density (BMD). Repeated-measures ANOVA was used to analyze the changes in lumbar BMD over time in each group and the texture parameters of diabetic rabbit lumbar marrow at different time points. Mann–Whitney U rank sum test was used to compare the differences of each index between the two groups and calculate the area under the curve (AUC).ResultsBMD was correlated with Ktrans, Kep, and Ve but not with Vp. At weeks 0–16, the BMD of the rabbits in the diabetic and normal groups was not statistically significant, but the change in BMD showed an overall downward trend. For texture analysis, entropy, energy, and Uniformized positive pixel (UPP) parameters extracted from the Ktrans map showed significant differences from week 0 to 16 between the two groups. The identification ability at 8–12 weeks was higher than that at 12–16 weeks, and the AUCs were 0.734, 0.766, and 0.734, respectively (P < 0.05 for all).ConclusionsThe changes in BMD measured using quantitative computed tomography occurred later than those measured using bone trabecular morphometry. Texture analysis parameters based on DCE-MRI quantitative parameter Ktrans map are feasible to identify early changes in lumbar marrow structure in diabetic rabbits.

Microvascular pressure, surface area, and permeability in isolated hindquarters of SHR

1985 ◽  
Vol 249 (3) ◽  
pp. H498-H504
Author(s):  
R. J. Korthuis ◽  
C. R. Kerr ◽  
M. I. Townsley ◽  
A. E. Taylor
Keyword(s):  
Skeletal Muscle ◽  
Capillary Pressure ◽  
Surface Area ◽  
Convective Transport ◽  
Microvascular Permeability ◽  
Spontaneously Hypertensive ◽  
Pressure Surface ◽  
Total Plasma Protein ◽  
Wistar Kyoto ◽  
Osmotic Reflection Coefficient

The transvascular escape rate (TER) of labeled albumin is reported to increase in essential hypertension. However, the mechanism for this augmented rate of protein efflux is uncertain and may be related to increased microvascular permeability, surface area, and/or pressure. To determine the possible contributions of these mechanisms to increased TER of protein, the osmotic reflection coefficient for total plasma protein, capillary filtration coefficient, and effective capillary pressure were estimated in isolated hindquarters of age-matched (12-13 wk) spontaneously hypertensive (SHR), Wistar-Kyoto (WKY), and Wistar (WR) rats. Estimates of the reflection and filtration coefficients were not significantly different in SHR, WKY, and WR. However, capillary pressure was significantly greater in SHR than in normotensive controls. These results indicate that 1) skeletal muscle microvascular permeability and surface area are similar in SHR, WKY, and WR; 2) effective capillary pressure is greater in SHR than WKY or WR; and 3) if TER for protein is elevated in hypertensive skeletal muscle, the primary mechanism for this process may be increased convective transport of protein secondary to elevated microvascular hydrostatic pressure.

Comparison of automated and visual texture analysis in MRI: Characterization of normal and diseased skeletal muscle

1999 ◽  
Vol 17 (9) ◽  
pp. 1393-1397 ◽  
Author(s):  
S. Herlidou ◽  
Y. Rolland ◽  
J.Y. Bansard ◽  
E. Le Rumeur ◽  
J.D. de Certaines
Keyword(s):  
Skeletal Muscle ◽  
Texture Analysis ◽  
Visual Texture

scholarly journals Skeletal Muscle Microvascular Permeability After Eccentric Contraction-Induced Muscle Injury

2018 ◽  
Vol 50 (5S) ◽  
pp. 143
Author(s):  
Kazuki Hotta ◽  
Brad J. Behnke ◽  
Kazuto Masamoto ◽  
Rie Shimotsu ◽  
David C. Poole ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
Eccentric Contraction ◽  
Microvascular Permeability

Regional transcapillary albumin exchange in rodent endotoxaemia: effects of fluid resuscitation and inhibition of nitric oxide synthase

2000 ◽  
Vol 100 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Suveer SINGH ◽  
Peter B. ANNING ◽  
C. Peter WINLOVE ◽  
Timothy W. EVANS
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Surface Area ◽  
Blood Volume ◽  
Fluid Resuscitation ◽  
Volume Ratio ◽  
Capillary Density ◽  
Microvascular Permeability ◽  
Capillary Perfusion ◽  
Dual Isotope

Sepsis is characterized by increased microvascular permeability and regional variations in capillary perfusion, which may be modulated by nitric oxide (NO) and reversed by fluid resuscitation (FR). The effects of saline FR and NO synthase blockade [by NG-nitro-L-arginine methyl ester (L-NAME)] on microvascular albumin transport and perfused capillary density were assessed in anaesthetized Wistar rats with acute normodynamic endotoxaemia. Separate dual-isotope techniques were employed to measure the permeability index (PIA) and the permeability×surface area product index (PIB), which provide different and complementary information regarding blood–tissue albumin exchange. PIA represents the tissue/blood distribution volume ratio of albumin. PIB is a composite measure of endothelial permeability and the vascular surface area available for albumin exchange, and therefore takes into account the effect of altered blood volume. Capillary density was quantified by fluorescence microscopy following circulation of Evans Blue-labelled albumin. Compared with controls, PIA was reduced significantly in lipopolysaccharide (LPS)-treated animals in skeletal muscle and skin, probably due to blood volume redistribution rather than to changes in permeability. PIB was increased significantly in LPS-treated animals in the kidney, mesentery, skeletal muscle, skin and lung, and in the small bowel following FR. FR also improved the LPS-induced metabolic base deficit, but did not alter capillary density. L-NAME significantly attenuated the LPS-induced rise in PIB in the lung. In conclusion, acute endotoxaemia induces tissue-dependent variations in microvascular albumin exchange. FR improves acid–base disturbance in endotoxaemia, through mechanisms other than microvascular recruitment. NO appears to increase microvascular permeability in endotoxaemia, an effect that may be attenuated by L-NAME, particularly in the lung.

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