Simple sequence length polymorphisms (SSLPs) that distinguish MOLF/Ei and 129/Sv inbred strains of laboratory mice

1998 ◽  
Vol 9 (8) ◽  
pp. 668-670 ◽  
Author(s):  
Angabin Matin ◽  
Gayle B. Collin ◽  
Yoshinobu Asada ◽  
Don Varnum ◽  
Diana L. Martone ◽  
...  
Keyword(s):  
Inbred Strains ◽  
Sequence Length ◽  
Laboratory Mice ◽  
Length Polymorphisms ◽  
Simple Sequence

Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains

1995 ◽  
Vol 6 (9) ◽  
pp. 595-601 ◽  
Author(s):  
M. Otsen ◽  
M. Den Bieman ◽  
E. S. Winer ◽  
H. J. Jacob ◽  
J. Szpirer ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Inbred Strains ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Simple Sequence

A genetic map of the mouse with 4,006 simple sequence length polymorphisms

1994 ◽  
Vol 7 (S2) ◽  
pp. 220-245 ◽  
Author(s):  
William F. Dietrich ◽  
Joyce C. Miller ◽  
Robert G. Steen ◽  
Mark Merchant ◽  
Deborah Damron ◽  
...  
Keyword(s):  
Genetic Map ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Simple Sequence

Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 715-723 ◽  
Author(s):  
P. J. Maughan ◽  
M. A. Saghai Maroof ◽  
G. R. Buss
Keyword(s):  
Genetic Markers ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Allelic Diversity ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Ssr Loci ◽  
Low Levels ◽  
Cultivated Soybean ◽  
Simple Sequence

The objectives of this study were to (i) assess the extent of genetic variation in soybean microsatellites (simple sequence repeats or SSRs), (ii) assay for amplified sequence length polymorphisms (ASLPs), and (iii) evaluate the usefulness of SSRs and ASLPs as genetic markers. Five microsatellites detected a total of 79 variants (alleles) in a sample of 94 accessions of wild (Glycine soja) and cultivated soybean (G. max). F2 segregation analysis of four of the five microsatellites identified these variants (alleles) with four loci located in independent linkage groups. The number of alleles per microsatellite locus ranged from 5 to 21; to our knowledge these are the largest numbers of alleles for single Mendelian loci reported in soybean. Allelic diversity for the SSR loci was greater in wild than in cultivated soybean. Overall, 43 more SSR alleles were detected in wild than in cultivated soybean. These results indicate that SSRs are the marker of choice, especially for species with low levels of variation as detected by other types of markers. Two alleles were detected at each of the three ASLP loci examined. A total of six ASLP alleles were observed in cultivated soybean and five were observed in wild soybean; all alleles detected in wild soybean were present in cultivated soybean. Allelic diversity values for the ASLP loci were near previous estimates for restriction fragment length polymorphisms and therefore ASLPs may be useful as genetic markers in site-directed mapping.Key words: microsatellite, simple sequence repeat, soybean, amplified sequence length polymorphism, genetic mapping.

scholarly journals PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

2001 ◽  
Vol 35 (2) ◽  
pp. 157-162 ◽  
Author(s):  
F. Benavides ◽  
E. Glasscock ◽  
L. G. Coghlan ◽  
M. C. Stern ◽  
D. A. Weiss ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Sequence Length ◽  
Mouse Strains ◽  
Mechanistic Studies ◽  
Chain Reaction ◽  
Dna Microsatellites ◽  
Multi Stage ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms

Development ◽  
1996 ◽  
Vol 123 (1) ◽  
pp. 451-460 ◽  
Author(s):  
E.W. Knapik ◽  
A. Goodman ◽  
O.S. Atkinson ◽  
C.T. Roberts ◽  
M. Shiozawa ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Markers ◽  
Genetic Linkage ◽  
Positional Cloning ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Sequence Length ◽  
Mapping Tool ◽  
Length Polymorphisms ◽  
Simple Sequence

The ultimate informativeness of the zebrafish mutations described in this issue will rest in part on the ability to clone these genes. However, the genetic infrastructure required for the positional cloning in zebrafish is still in its infancy. Here we report a reference cross panel of DNA, consisting of 520 F2 progeny (1040 meioses) that has been anchored to a zebrafish genetic linkage map by 102 simple sequence length polymorphisms. This reference cross DNA provides: (1) a panel of DNA from the cross that was used to construct the genetic linkage map, upon which polymorphic gene(s) and genetic markers can be mapped; (2) a fine order mapping tool, with a maximum resolution of 0.1 cM; and (3) a foundation for the development of a physical map (an ordered array of clones each containing a known portion of the genome). This reference cross DNA will serve as a resource enabling investigators to relate genes or genetic markers directly to a single genetic linkage map and avoid the problem of integrating different maps with different genetic markers, as must be currently done when using randomly amplified polymorphic DNA markers, or as has occurred with human genetic linkage maps.

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