Temporal and spatial patterns of gene expression in skeletal muscles in response to swim training in adult zebrafish (Danio rerio)

2009 ◽  
Vol 180 (1) ◽  
pp. 151-160 ◽  
Author(s):  
Christophe M. R. LeMoine ◽  
Paul M. Craig ◽  
Kalindi Dhekney ◽  
Julie J. Kim ◽  
Grant B. McClelland
2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Huikang Tao ◽  
Pei Xie ◽  
Yuhang Cao ◽  
Liqi Shu ◽  
Liping Li ◽  
...  

Background. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. Results. It was found that 5hmC, 5fC, and 5caC were highly enriched in multiple brain regions and aNSCs and displayed temporal and spatial patterns during postnatal neuronal development and the differentiation of aNSCs. Consistently, the expression of Tets also exhibited temporal and spatial patterns. Conclusion. DNA demethylation displayed dynamic features during postnatal neuronal development and the differentiation of aNSCs of mice, which could contribute to appropriate gene expression.


1995 ◽  
Vol 171 (2) ◽  
pp. 374-385 ◽  
Author(s):  
Keiji Inohaya ◽  
Shigeki Yasumasu ◽  
Mika Ishimaru ◽  
Akihiro Ohyama ◽  
Ichiro Iuchi ◽  
...  

1999 ◽  
Vol 16 (3) ◽  
pp. 601-605 ◽  
Author(s):  
ELLEN A. SCHMITT ◽  
GEORGE A. HYATT ◽  
JOHN E. DOWLING

We report here a reexamination of the developmental expression of cone opsins in the zebrafish retina. The red- and blue-sensitive opsins appear at 51 h postfertilization (hpf) whereas ultraviolet (UV) opsin is not seen until after 55 hpf. More cells show red cone opsin expression than blue at 51 and 55 hpf, indicating the sequence of cone opsin expression in zebrafish is first red, then blue, and finally UV. Curiously, morphological development of the cones is in reverse order; UV cones appear quite mature by day 6–7 postfertilization (pf), but morphologically, red cones do not appear adult-like until 15–20 days pf.


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