Evolution of patterns of differential gene expression: A comparison of the temporal and spatial patterns of lsozyme locus expression in two closely related fish species (northern largemouth bass,Micropterus salmoides salmoides, and Smallmouth Bass,Micropterus dolomieui)

1979 ◽  
Vol 210 (3) ◽  
pp. 473-487 ◽  
Author(s):  
David P. Philipp ◽  
William F. Childers ◽  
Gregory S. Whitt
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Spatial Patterns ◽  
Fish Species ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Smallmouth Bass ◽  
Temporal And Spatial Patterns ◽  
Differential Gene ◽  
Temporal And Spatial

scholarly journals The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Huikang Tao ◽  
Pei Xie ◽  
Yuhang Cao ◽  
Liqi Shu ◽  
Liping Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spatial Patterns ◽  
Neuronal Development ◽  
Stem Cell Differentiation ◽  
Brain Regions ◽  
Dna Demethylation ◽  
Dynamic Features ◽  
Dot Blot ◽  
Temporal And Spatial Patterns ◽  
Temporal And Spatial

Background. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. Results. It was found that 5hmC, 5fC, and 5caC were highly enriched in multiple brain regions and aNSCs and displayed temporal and spatial patterns during postnatal neuronal development and the differentiation of aNSCs. Consistently, the expression of Tets also exhibited temporal and spatial patterns. Conclusion. DNA demethylation displayed dynamic features during postnatal neuronal development and the differentiation of aNSCs of mice, which could contribute to appropriate gene expression.

scholarly journals Temporal and Spatial Patterns of Gene Expression for the Hatching Enzyme in the Teleost Embryo, Oryzias latipes

1995 ◽  
Vol 171 (2) ◽  
pp. 374-385 ◽  
Author(s):  
Keiji Inohaya ◽  
Shigeki Yasumasu ◽  
Mika Ishimaru ◽  
Akihiro Ohyama ◽  
Ichiro Iuchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spatial Patterns ◽  
Oryzias Latipes ◽  
Hatching Enzyme ◽  
Temporal And Spatial Patterns ◽  
Temporal And Spatial

scholarly journals Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9614
Author(s):  
Jessica K. Leet ◽  
Catherine A. Richter ◽  
Robert S. Cornman ◽  
Jason P. Berninger ◽  
Ramji K. Bhandari ◽  
...  
Keyword(s):  
Gene Expression ◽  
Early Development ◽  
Largemouth Bass ◽  
Early Life ◽  
Micropterus Salmoides ◽  
Life Stage ◽  
Early Life Stage ◽  
Smallmouth Bass ◽  
Gonad Tissue ◽  
And Function

Endocrine disrupting contaminants are of continuing concern for potentially contributing to reproductive dysfunction in largemouth and smallmouth bass in the Chesapeake Bay watershed (CBW) and elsewhere. Exposures to atrazine (ATR) have been hypothesized to have estrogenic effects on vertebrate endocrine systems. The incidence of intersex in male smallmouth bass from some regions of CBW has been correlated with ATR concentrations in water. Fish early life stages may be particularly vulnerable to ATR exposure in agricultural areas, as a spring influx of pesticides coincides with spawning and early development. Our objectives were to investigate the effects of early life stage exposure to ATR or the model estrogen 17α-ethinylestradiol (EE2) on sexual differentiation and gene expression in gonad tissue. We exposed newly hatched largemouth bass (LMB, Micropterus salmoides) from 7 to 80 days post-spawn to nominal concentrations of 1, 10, or 100 µg ATR/L or 1 or 10 ng EE2/L and monitored histological development and transcriptomic changes in gonad tissue. We observed a nearly 100% female sex ratio in LMB exposed to EE2 at 10 ng/L, presumably due to sex reversal of males. Many gonad genes were differentially expressed between sexes. Multidimensional scaling revealed clustering by gene expression of the 1 ng EE2/L and 100 µg ATR/L-treated male fish. Some pathways responsive to EE2 exposure were not sex-specific. We observed differential expression in male gonad in LMB exposed to EE2 at 1 ng/L of several genes involved in reproductive development and function, including star, cyp11a2, ddx4 (previously vasa), wnt5b, cyp1a and samhd1. Expression of star, cyp11a2 and cyp1a in males was also responsive to ATR exposure. Overall, our results confirm that early development is a sensitive window for estrogenic endocrine disruption in LMB and are consistent with the hypothesis that ATR exposure induces some estrogenic responses in the developing gonad. However, ATR-specific and EE2-specific responses were also observed.

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