High Fib4 index in patients with suspected NASH is associated with elevation of chymase-dependent angiotensin II-forming activity in circulating mononuclear leucocytes

2019 ◽  
Vol 34 (9) ◽  
pp. 1559-1569
Author(s):  
Keisuke Okamura ◽  
Tetsu Okuda ◽  
Yosuke Takamiya ◽  
Kazuyuki Shirai ◽  
Hidenori Urata
1995 ◽  
Vol 42 (1) ◽  
pp. 15-22 ◽  
Author(s):  
HIROMICHI SUZUKI ◽  
HIROTAKA SHIBATA ◽  
MAROHITO MURAKAMI ◽  
ATSUHISA SATO ◽  
MAREO NAITO ◽  
...  

Author(s):  
Etienne de Harven ◽  
Nina Lampen

Samples of heparinized blood, or bone marrow aspirates, or cell suspensions prepared from biopsied tissues (nodes, spleen, etc. ) are routinely prepared, after Ficoll-Hypaque concentration of the mononuclear leucocytes, for scanning electron microscopy. One drop of the cell suspension is placed in a moist chamber on a poly-l-lysine pretreated plastic coverslip (Mazia et al., J. Cell Biol. 66:198-199, 1975) and fifteen minutes allowed for cell attachment. Fixation, started in 2. 5% glutaraldehyde in culture medium at room temperature for 30 minutes, is continued in the same fixative at 4°C overnight or longer. Ethanol dehydration is immediately followed by drying at the critical point of CO2 or of Freon 13. An efficient alternative method for ethanol dehydrated cells is to dry the cells at low temperature (-75°C) under vacuum (10-2 Torr) for 30 minutes in an Edwards-Pearse freeze-dryer (de Harven et al., SEM/IITRI/1977, 519-524). This is preceded by fast quenching in supercooled ethanol (between -90 and -100°C).


2001 ◽  
Vol 268 (6) ◽  
pp. 1802-1810
Author(s):  
Danielle Naville ◽  
Estelle Bordet ◽  
Marie-Claude Berthelon ◽  
Philippe Durand ◽  
Martine Begeot

2006 ◽  
Vol 5 (1) ◽  
pp. 83-83
Author(s):  
M GUERRA ◽  
P CASTROCHAVES ◽  
C BRASSILVA ◽  
A LEITEMOREIRA

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