Characteristics of death of neonatal rat cardiomyocytes following hypoxia or hypoxia-reoxygenation: the association of apoptosis and cell membrane disintegrity

2002 ◽  
Vol 16 (6) ◽  
pp. 241-248 ◽  
Author(s):  
Reiko Matsuoka ◽  
Kazuei Ogawa ◽  
Hiroyuki Yaoita ◽  
Wakako Naganuma ◽  
Kazuhira Maehara ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Hypoxia Reoxygenation

Myofibrillogenesis regulator-1 attenuates hypoxia/reoxygenation-induced injury by repairing microfilaments in neonatal rat cardiomyocytes

2015 ◽  
Vol 337 (2) ◽  
pp. 234-242 ◽  
Author(s):  
Tianqi Tao ◽  
Xiaoreng Wang ◽  
Mi Liu ◽  
Xiuhua Liu
Keyword(s):  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Hypoxia Reoxygenation

Comparison of cardioprotective effects of labeled and unlabeled oleanoic acids with new BOPIM dye on primary neonatal rat cardiomyocytes following hypoxia/reoxygenation injury

2014 ◽  
Vol 66 (4) ◽  
pp. 677-685 ◽  
Author(s):  
Sa Wang ◽  
Hai-bo He ◽  
Shu-zhang Xiao ◽  
Jun-zhi Wang ◽  
Cai-hong Bai ◽  
...  
Keyword(s):  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Reoxygenation Injury ◽  
Cardioprotective Effects ◽  
Hypoxia Reoxygenation

scholarly journals Beta-1-Adrenergic Receptors Mediate Nrf2-HO-1-HMGB1 Axis Regulation to Attenuate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury in Vitro

2015 ◽  
Vol 35 (2) ◽  
pp. 767-777 ◽  
Author(s):  
Jichun Wang ◽  
Xiaorong Hu ◽  
Jing Xie ◽  
Weipan Xu ◽  
Hong Jiang
Keyword(s):  
Adrenergic Receptors ◽  
Pi3k Inhibitor ◽  
Neonatal Rat ◽  
Control Group ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Nf Kappab ◽  
Cultured Cardiomyocytes ◽  
Hypoxia Reoxygenation

Backgroud/Aims: The aim of the study was to evaluate the effects of beta1-adrenergic receptors (β1-ARs) -mediated nuclear factor erythroid 2-related factor 2 (Nrf2)-heme oxygenase-1 (HO-1)-high mobility group box 1 protein (HMGB1) axis regulation in hypoxia/reoxygenation (H/R)-induced neonatal rat cardiomyocytes. Methods: The neonatal cultured cardiomyocytes were concentration-dependently pretreated by dobutamine (DOB), a selective β1-adrenergic receptor agonist, in the absence and/or presence of LY294002 (a phosphatidylinositol 3-kinase (PI3K) inhibitor), SB203580 (a p38mitogen-activated-protein kinase (p38MAPK) inhibitor), Nrf2siRNA and HO-1siRNA, respectively, and then treated by H/R. The effects and mechanisms by which H/R-induced cardiomyocytes injury were evaluated. Results: Significant increase of HO-1 was found in neonatal cultured cardiomyocytes treated with DOB, when compared to the control group. Significant change for Nrf2 translocation was also revealed in neonatal cultured cardiomyocytes treated with DOB. Insignificant decreases of NF-kappaB p65 activation and HMGB1 release were observed in H/R-induced neonatal cultured cardiomyocytes treated with DOB, when compared to the control group. Importantly, DOB treatment significantly increased the cell viability and decreased the levels of LDH and MDA in H/R-induced cardiomyocytes injury. However, DOB failed to increase HO-1, inhibit NF-kappaB p65 activation, prevent HMGB1 release and attenuate H/R-induced cardiomyocytes injury when the cultured cardiomyocytes were pretreated by Nrf2siRNA, HO-1siRNA, PI3K inhibitor (LY294002) and p38MAPK inhibitor (SB203580), respectively. Conclusions: β1-ARs-mediated Nrf2-HO-1-HMGB1 axis regulation plays a critical protective role in H/R-induced neonatal rat cardiomyocytes injury in vitro via PI3K/p38MAPK signaling pathway.

Active uptake of hydrophilic copper complex Cu(ii)–TETA in primary cultures of neonatal rat cardiomyocytes

Metallomics ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 565-575 ◽  
Author(s):  
Chunyan Fu ◽  
Junhong Lizhao ◽  
Zhenghui Luo ◽  
Tao Wang ◽  
Craig A. Grapperhaus ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Copper Complex ◽  
Primary Cultures ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Active Uptake ◽  
Neonatal Rat Cardiomyocytes ◽  
Independent Process

Cu(ii)–TETA is transported across the cell membrane via an unknown ATP-dependent and CTR1-independent process.

The protective effect of picroside II against hypoxia/reoxygenation injury in neonatal rat cardiomyocytes

2012 ◽  
Vol 50 (10) ◽  
pp. 1226-1232 ◽  
Author(s):  
Fan-Ji Meng ◽  
Zhi-Wen Hou ◽  
Yang Li ◽  
Ying Yang ◽  
Bo Yu
Keyword(s):  
Protective Effect ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Picroside Ii ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

Myofibrillogenesis regulator-1 attenuated hypoxia/reoxygenation-induced apoptosis by inhibiting the PERK/Nrf2 pathway in neonatal rat cardiomyocytes

APOPTOSIS ◽  
2014 ◽  
Vol 20 (3) ◽  
pp. 285-297 ◽  
Author(s):  
Tian-Qi Tao ◽  
Xiao-Reng Wang ◽  
Mi Liu ◽  
Fei-Fei Xu ◽  
Xiu-Hua Liu
Keyword(s):  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Nrf2 Pathway ◽  
Neonatal Rat Cardiomyocytes ◽  
Induced Apoptosis ◽  
Hypoxia Reoxygenation

Comparison of the Degree of Autophagy in Neonatal Rat Cardiomyocytes and H9c2 Cells Exposed to Hypoxia/Reoxygenation

2014 ◽  
Vol 60 (05/2014) ◽  
Author(s):  
Xueming Cao ◽  
Xianbao Wang ◽  
Yuanna Ling ◽  
Xudong Song ◽  
Pingzhen Yang ◽  
...  
Keyword(s):  
Neonatal Rat ◽  
H9c2 Cells ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Hypoxia Reoxygenation
Sign in / Sign up

Export Citation Format

Share Document