Active uptake of hydrophilic copper complex Cu(ii)–TETA in primary cultures of neonatal rat cardiomyocytes

Metallomics ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 565-575 ◽  
Author(s):  
Chunyan Fu ◽  
Junhong Lizhao ◽  
Zhenghui Luo ◽  
Tao Wang ◽  
Craig A. Grapperhaus ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Copper Complex ◽  
Primary Cultures ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Active Uptake ◽  
Neonatal Rat Cardiomyocytes ◽  
Independent Process

Cu(ii)–TETA is transported across the cell membrane via an unknown ATP-dependent and CTR1-independent process.

The Involvement of Cytochrome c Oxidase in Mitochondrial Fusion in Primary Cultures of Neonatal Rat Cardiomyocytes

2018 ◽  
Vol 18 (4) ◽  
pp. 365-373 ◽  
Author(s):  
Wen Yin ◽  
Rui Li ◽  
Xiaorong Feng ◽  
Y. James Kang
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Primary Cultures ◽  
Mitochondrial Fusion ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

Characteristics of death of neonatal rat cardiomyocytes following hypoxia or hypoxia-reoxygenation: the association of apoptosis and cell membrane disintegrity

2002 ◽  
Vol 16 (6) ◽  
pp. 241-248 ◽  
Author(s):  
Reiko Matsuoka ◽  
Kazuei Ogawa ◽  
Hiroyuki Yaoita ◽  
Wakako Naganuma ◽  
Kazuhira Maehara ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Hypoxia Reoxygenation

scholarly journals Curcumin Protects Neonatal Rat Cardiomyocytes against High Glucose-Induced Apoptosis via PI3K/Akt Signalling Pathway

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Wei Yu ◽  
Wenliang Zha ◽  
Zhiqiang Ke ◽  
Qing Min ◽  
Cairong Li ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
High Glucose ◽  
Primary Cultures ◽  
Signalling Pathway ◽  
Neonatal Rat ◽  
Curcumin Treatment ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Enhanced Expression ◽  
Induced Apoptosis

The function of curcumin on NADPH oxidase-related ROS production and cardiac apoptosis, together with the modulation of protein signalling pathways, was investigated in cardiomyocytes. Primary cultures of neonatal rat cardiomyocytes were exposed to 30 mmol/L high glucose with or without curcumin. Cell viability, apoptosis, superoxide formation, the expression of NADPH oxidase subunits, and potential regulatory molecules, Akt and GSK-3β, were assessed in cardiomyocytes. Cardiomyocytes exposure to high glucose led to an increase in both cell apoptosis and intracellular ROS levels, which were strongly prevented by curcumin treatment (10 μM). In addition, treatment with curcumin remarkably suppressed the increased activity of Rac1, as well as the enhanced expression ofgp91phoxandp47phoxinduced by high glucose. Lipid peroxidation and SOD were reversed in the presence of curcumin. Furthermore, curcumin treatment markedly inhibited the reduced Bcl-2/Bax ratio elicited by high glucose exposure. Moreover, curcumin significantly increased Akt and GSK-3βphosphorylation in cardiomyocytes treated with high glucose. In addition, LY294002 blocked the effects of curcumin on cardiomyocytes exposure to high glucose. In conclusion, these results demonstrated that curcumin attenuated high glucose-induced cardiomyocyte apoptosis by inhibiting NADPH-mediated oxidative stress and this protective effect is most likely mediated by PI3K/Akt-related signalling pathway.

scholarly journals Oxytocin Increases Glucose Uptake in Neonatal Rat Cardiomyocytes

Endocrinology ◽  
2010 ◽  
Vol 151 (2) ◽  
pp. 482-491 ◽  
Author(s):  
Maria Florian ◽  
Marek Jankowski ◽  
Jolanta Gutkowska
Keyword(s):  
Glucose Uptake ◽  
Glucose Transporter ◽  
Primary Cultures ◽  
Metabolic Stress ◽  
Neonatal Rat ◽  
Stem Cell Marker ◽  
Rat Cardiomyocytes ◽  
Myocardial Glucose Uptake ◽  
Neonatal Rat Cardiomyocytes ◽  
Basal Glucose Uptake

We have recently shown that an entire oxytocin (OT) system, a peptide and its cognate receptors, is synthesized in the heart. In fetal and newborn hearts, OT exists in its extended three-amino acid form, OT-Gly-Lys-Arg (OT-GKR). OT translocates glucose transporter type 4 to the plasma membrane in human endothelial cells. Therefore, we hypothesized that the cardiac OT/OT-GKR system may be involved in the regulation of myocardial glucose uptake in physiological conditions and during metabolic stress such as hypoxia. Primary cultures of neonatal rat cardiomyocytes (CM) and cardiac progenitor cells expressing ATP-binding cassette efflux transporter G2 transporter (stem cell marker) were studied. OT (10 nm) increased basal glucose uptake in CM to 4.0 ± 0.2 fmol/mg protein, with OT-GKR (10 nm) elevating it to 5.3 ± 0.4 fmol/mg protein (P < 0.001) in comparison with 2.2 fmol/mg in control cells. OT had a moderate synergistic effect with 0.1 mm 2,4-dinitrophenol, augmenting basal glucose uptake to 5.5 ± 0.5 fmol/mg. OT-GKR (10 nm) was even more potent in combination with 2,4-dinitrophenol, increasing glucose uptake to 9.0 ± 1.0 fmol/mg. Wortmannin (0.1 μm), an inhibitor of phosphatidylinositol-3-kinase, significantly suppressed the effect of OT and insulin (10 nm) (P < 0.001), indicating common pathways. Our data suggest that OT and OT-GKR influence glucose uptake in neonatal rat CM and may thus play a role in the maintenance of cardiac function and cell survival during metabolic stress.

scholarly journals Aldosterone, but Not Angiotensin II, Reduces Angiotensin Converting Enzyme 2 Gene Expression Levels in Cultured Neonatal Rat Cardiomyocytes

2008 ◽  
Vol 72 (8) ◽  
pp. 1346-1350 ◽  
Author(s):  
Megumi Yamamuro ◽  
Michihiro Yoshimura ◽  
Masafumi Nakayama ◽  
Koji Abe ◽  
Hitoshi Sumida ◽  
...  
Keyword(s):  
Gene Expression ◽  
Angiotensin Ii ◽  
Angiotensin Converting Enzyme ◽  
Neonatal Rat ◽  
Converting Enzyme ◽  
Rat Cardiomyocytes ◽  
Angiotensin Converting Enzyme 2 ◽  
Expression Levels ◽  
Neonatal Rat Cardiomyocytes ◽  
Gene Expression Levels

Oxidative stress enhances phosphorylation of p53 in neonatal rat cardiomyocytes

2007 ◽  
Vol 303 (1-2) ◽  
pp. 167-174 ◽  
Author(s):  
Xilin Long ◽  
Michael J. Goldenthal ◽  
José Marín-García
Keyword(s):  
Oxidative Stress ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

Myofibrillogenesis regulator-1 attenuates hypoxia/reoxygenation-induced injury by repairing microfilaments in neonatal rat cardiomyocytes

2015 ◽  
Vol 337 (2) ◽  
pp. 234-242 ◽  
Author(s):  
Tianqi Tao ◽  
Xiaoreng Wang ◽  
Mi Liu ◽  
Xiuhua Liu
Keyword(s):  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Hypoxia Reoxygenation
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