In vivo biological performance of composites combining micro-macroporous biphasic calcium phosphate granules and fibrin sealant

HA/ß-TCP is one kind of important bone tissue engineering scaffold materials. In order to improve the biological performance of materials, HA/ß-TCP ceramics were modified by the cold plasma technique in this paper. The biological performances of treated materials were evaluated by the results of bone-like apatite formation in SBF, the coculture of the C2C12 cell line and HA/ß-TCP, cell proliferation experiments, observations of the electron microscopy and fluorescence staining and the animal intramuscular implantation experiments. The results showed that after immersion, compared with untreated HA/ß-TCP, treated materials were more conducive to form bone-like apatite; modified HA/ß-TCP could promote the cell proliferation more; the cells grew in the course of nature on the treated scaffold and modified HA/ß-TCP had better bone-forming performance in vivo. It was concluded that modified HA/ß-TCP had better biological performance; the cold plasma technique could improve the biological performance of dental biphasic calcium phosphate ceramics.

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Biphasic Calcium Phosphate Biomaterials: Stem Cell-Derived Osteoinduction or In Vivo Osteoconduction? Novel Insights in Maxillary Sinus Augmentation by Advanced Imaging

Materials ◽

10.3390/ma14092159 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2159

Author(s):

Giovanna Iezzi ◽

Antonio Scarano ◽

Luca Valbonetti ◽

Serena Mazzoni ◽

Michele Furlani ◽

...

Keyword(s):

Calcium Phosphate ◽

Maxillary Sinus ◽

Biphasic Calcium Phosphate ◽

Calcium Phosphates ◽

Three Dimensional ◽

Sinus Augmentation ◽

Native Bone ◽

Maxillary Sinus Augmentation ◽

Posterior Maxilla

Maxillary sinus augmentation is often necessary prior to implantology procedure, in particular in cases of atrophic posterior maxilla. In this context, bone substitute biomaterials made of biphasic calcium phosphates, produced by three-dimensional additive manufacturing were shown to be highly biocompatible with an efficient osteoconductivity, especially when combined with cell-based tissue engineering. Thus, in the present research, osteoinduction and osteoconduction properties of biphasic calcium-phosphate constructs made by direct rapid prototyping and engineered with ovine-derived amniotic epithelial cells or amniotic fluid cells were evaluated. More in details, this preclinical study was performed using adult sheep targeted to receive scaffold alone (CTR), oAFSMC, or oAEC engineered constructs. The grafted sinuses were explanted at 90 days and a cross-linked experimental approach based on Synchrotron Radiation microCT and histology analysis was performed on the complete set of samples. The study, performed taking into account the distance from native surrounding bone, demonstrated that no significant differences occurred in bone regeneration between oAEC-, oAFMSC-cultured, and Ctr samples and that there was a predominant action of the osteoconduction versus the stem cells osteo-induction. Indeed, it was proven that the newly formed bone amount and distribution decreased from the side of contact scaffold/native bone toward the bulk of the scaffold itself, with almost constant values of morphometric descriptors in volumes more than 1 mm from the border.

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