Magnetic resonance imagining (MRI) and serial β-human chorionic gonadotrophin (β-hCG) follow up for placenta percreta

Abstract Objectives Level of βhCG and the presence of any uterine mass of hydatidiform mole need a careful review or monitoring in order to prevent metastasis, provide an early treatment and avoid unnecessary chemotherapy. Case presentation A 36-year old fifth gravida patient who had a missed abortion was diagnosed as having a molar pregnancy with beta human chorionic gonadotrophin (βhCG) level of 509,921 IU/L. Her lung field was clear and she underwent suction and curettage (S & C) procedure. However, after six weeks, AA presented to the emergency department with a massive bleeding, although her βhCG level had decreased to 65,770 IU/L. A trans-abdominal ultrasound indicated the presence of an intra-uterine mass (3.0 × 4.4 cm). Nevertheless, her βhCG continued to show a declining trend (8,426 IU/L). AA was advised to undergo a chemotherapy but she refused, citing preference for alternative medicine like herbs instead. She opted for an “at own risk” (AOR) discharge with scheduled follow up. Subsequently, her condition improved with her βhCG showing a downward trend. Surprisingly, at six months post S & C, her βhCG ameliorated to 0 IU/L with no mass detected by ultrasound. Conclusions Brucea javanica fruits, Pereskia bleo and Annona muricata leaves can potentially be useful alternatives to chemotherapy and need further studies.

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Immunochemical analysis of the human chorionic gonadotrophin-like material secreted by 'normal' and neoplastic urothelial cells

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0020107 ◽

1989 ◽

Vol 2 (2) ◽

pp. 107-112 ◽

Cited By ~ 17

Author(s):

R. K. Iles ◽

T. Chard

Keyword(s):

Bladder Tumour ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Sds Page ◽

Β Hcg ◽

Bladder Carcinomas ◽

Immunochemical Characteristics ◽

Normal Urothelium

ABSTRACT Material with the immunochemical characteristics of human chorionic gonadotrophin (hCG) is produced by bladder tumour cells in vitro and in vivo. In order to characterize this material further, media were collected from 17 cell cultures (three choriocarcinomas, seven bladder carcinomas and seven 'normal' urothelium). The hCG-like material was compared with pregnancy hCG and purified α- and β-subunits by specific radioimmunoassays. Media were also submitted to affinity chromatography and the fractions further analysed by SDS-PAGE and Western blotting. It was shown that both the neoplastic and normal urothelium produced only free β-subunit-like material. This urothelial 'β-hCG' has the same molecular weight and electrophoretic mobility as that present in the intact hCG of pregnancy.

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Human chorionic gonadotrophin raises serum immunoreactive inhibin levels in men with hypogonadotrophic hypogonadism

Reproduction Fertility and Development ◽

10.1071/rd9900137 ◽

1990 ◽

Vol 2 (2) ◽

pp. 137 ◽

Cited By ~ 8

Author(s):

HG Burger ◽

SC Tiu ◽

ML Bangah ◽

Kretser DM de

Keyword(s):

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

The Other ◽

Glycoprotein Hormone ◽

Hypogonadotrophic Hypogonadism

Inhibin is a gonadal glycoprotein hormone involved in the regulation of FSH. To elucidate the regulation of inhibin production we investigated the acute (daily for 1 week) and chronic (9-10 months of follow-up) changes in immunoreactive inhibin, testosterone, LH and FSH levels in the serum of three hypogonadotrophic hypogonadal patients treated first with hCG alone (for 3-6 months) and then hCG combined with FSH (1-5 months). One patient was unexpectedly resistant to gonadotrophin therapy; in the other two, hCG, with or without FSH, caused a rise in inhibin and testosterone, supporting previous observations that LH, as well as FSH, plays a role in the regulation of inhibin or inhibin-related peptides in men.

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Performance of free β-human chorionic gonadotrophin (free β-hCG) and pregnancy associated plasma protein-A (PAPP-A) analysis between Delfia Xpress and AutoDelfia systems in The Netherlands

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2009.042 ◽

2009 ◽

Vol 47 (2) ◽

Cited By ~ 9

Author(s):

Ingeborg H. Linskens ◽

Marieke Levitus ◽

Anneke Frans ◽

Peter C.J.I. Schielen ◽

John M.G. van Vugt ◽

...

Keyword(s):

The Netherlands ◽

Plasma Protein ◽

Screening Program ◽

Medical Center ◽

Protein A ◽

First Trimester ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Serum Samples ◽

Β Hcg

Abstract: The VU University Medical Center (VUmc) was the first hospital in the Netherlands to introduce the Delfia Xpress for the analysis of free β-human chorionic gonadotrophin (β-hCG) and pregnancy associated plasma protein-A (PAPP-A) in the first trimester screening program for Down syndrome. Since then, others have implemented this system. In this study, we tested the equality of measurements for free β-hCG and PAPP-A between Delfia Xpress systems and one AutoDelfia system.: A total of 40 serum samples were aliquoted and stored at –20°C. Samples were analyzed by six Delfia Xpress systems and one AutoDelfia system over a time period of 2 years.: The relationships between free β-hCG and PAPP-A were excellent for the different Delfia Xpress systems (r>0.99, p<0.0001). For PAPP-A, the agreement between the main system at VUmc and five other systems was linear with slopes between 0.99 and 1.06. Similarly, agreement for free β-hCG was linear with slopes between 0.99 and 1.09. Likewise, agreement for PAPP-A and free β-hCG was excellent for the AutoDelfia vs. the main Delfia Xpress at the VUmc (r>0.99, p<0.0001). For both PAPP-A and free β-hCG, the relationships were linear with slopes of 1.08 and 1.07.: We demonstrate an excellent agreement for the analysis of PAPP-A and free β-hCG between Delfia Xpress systems and one AutoDelfia system.Clin Chem Lab Med 2009;47:222–6.

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An assessment of combined tumour markers in patients with seminoma: placental alkaline phosphatase (PLAP), lactate dehydrogenase (LD) and β human chorionic gonadotrophin (β HCG)

British Journal of Cancer ◽

10.1038/bjc.1991.346 ◽

1991 ◽

Vol 64 (3) ◽

pp. 537-542 ◽

Cited By ~ 9

Author(s):

AJ Munro ◽

OS Nielsen ◽

W Duncan ◽

J Sturgeon ◽

MK Gospodarowicz ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Lactate Dehydrogenase ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Tumour Markers ◽

Placental Alkaline Phosphatase ◽

Β Hcg

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A transcervical amniotic fluid collector: a new medical device for the assessment of amniotic fluid in patients with ruptured membranes

Journal of Perinatal Medicine ◽

10.1515/jpm-2014-0276 ◽

2015 ◽

Vol 43 (4) ◽

Cited By ~ 4

Author(s):

Seung Mi Lee ◽

Roberto Romero ◽

Joong Shin Park ◽

Piya Chaemsaithong ◽

Jong Kwan Jun ◽

...

Keyword(s):

Amniotic Fluid ◽

Medical Device ◽

Strong Correlation ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

New Device ◽

High Concentrations ◽

Β Hcg ◽

Prelabor Rupture Of Membranes

AbstractTo describe a new device for the transcervical collection of amniotic fluid (AF) in patients with ruptured membranes, and to compare the concentration of proteins in fluid retrieved by transabdominal amniocentesis and the transcervical AF collector.Paired AF samples were collected in patients with preterm prelabor rupture of membranes (PROM) (n=11) by transabdominal amniocentesis and with the transcervical AF collector (Yoon’s AF Collector™). Three proteins known to have high concentrations in AF [α-fetoprotein (AFP), β-human chorionic gonadotrophin (β-hCG), and prolactin] were measured.(1) There was a significant correlation between the concentrations of analytes in AF obtained by transabdominal amniocentesis and by the transcervical AF collector (r=0.94, P<0.001 for AFP; r=0.96, P<0.001 for β-hCG; r=0.72, P<0.05 for prolactin); (2) Bland-Altman plots showed no evidence of heteroscedasticity between transabdominal or transcervical AF concentrations of these markers.There was a strong correlation between the concentrations of proteins in AF collected by amniocentesis or with the transcervical device.

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Human chorionic gonadotrophin and subunit composition of maternal serum and coelomic and amniotic fluids in the first trimester of pregnancy

Journal of Endocrinology ◽

10.1677/joe.0.1350563 ◽

1992 ◽

Vol 135 (3) ◽

pp. 563-569 ◽

Cited By ~ 11

Author(s):

R. K. Iles ◽

N. C. Wathen ◽

D. J. Campbell ◽

T. Chard

Keyword(s):

First Trimester ◽

Maternal Serum ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Subunit Composition ◽

Major Constituent ◽

Coelomic Fluid ◽

Α Subunit ◽

First Trimester Of Pregnancy ◽

Β Hcg

ABSTRACT Sixteen matched samples of first trimester amniotic fluid (AF), extraembryonic coelomic fluid (EECF) and maternal serum (MS) were assayed for intact human chorionic gonadotrophin (hCG) and free subunits. Total β-hCG (free β-subunit and intact hCG) levels in the EECF (median 410 kIU/l) were 61 times greater than levels in AF (median 6·73 kIU/l) and 2·8 times greater than in MS (median 141·5 kIU/l). Levels of intact hCG in the EECF (median 245 kIU/l) were 142 times greater than in AF (median 1·73 kIU/l) and 1·6 times greater than in MS (median 157 kIU/l). Free α-subunit levels in EECF (median 17·3 mg/l) were 66 times greater than in AF (median 0·262 mg/l) and 12 times greater than in MS (median 1·3 mg/l). Virtually all of the total β-hCG immunoreactivity in MS can be attributed to intact hCG, but only 60% of total β-hCG in the EECF and 20% of that in AF can be accounted for by the intact hormone. In both EECF and AF the free α-subunit was a major constituent; on a molar basis the ratio of free α:free β:intact hCG was 1:1·2:0·3 in AF, 1:0·6:0·5 in EECF and 1:0:5 in MS. Chromatography of MS, EECF and AF on Sephadex G-100 confirmed the hCG and subunit composition of the fluids. On the basis of these findings it seems likely that previous studies showing very high levels of hCG in AF during the first trimester may have incorrectly sampled the EECF. In reality, the levels of total hCG (and free subunits) are low in the AF, and only 20% is intact hCG. In both AF and EECF the free subunits may have been derived by dissociation of intact hormone, or possibly by independent synthesis. These and other findings suggest that either the amnion acts as a barrier to the transfer of proteins or that there may be dynamic removal from this compartment. By contrast, the EECF might act as a relatively stable reservoir for these proteins. Journal of Endocrinology (1992) 135, 563–569

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