Glass-funnel technique for the recording of membrane currents and intracellular perfusion of Xenopus oocytes

1996 ◽  
Vol 432 (3) ◽  
pp. 562-570 ◽  
Author(s):  
Yaroslav M. Shuba ◽  
Valery G. Naidenov ◽  
Martin Morad
Keyword(s):  
Xenopus Oocytes ◽  
Membrane Currents ◽  
Intracellular Perfusion ◽  
Funnel Technique

scholarly journals Membrane currents elicited by divalent cations in Xenopus oocytes.

1989 ◽  
Vol 417 (1) ◽  
pp. 173-195 ◽  
Author(s):  
R Miledi ◽  
I Parker ◽  
R M Woodward
Keyword(s):  
Xenopus Oocytes ◽  
Divalent Cations ◽  
Membrane Currents

Membrane currents elicited by porcine vasoactive intestinal peptide (VIP) in follicle-enclosed Xenopus oocytes

1987 ◽  
Vol 231 (1265) ◽  
pp. 489-497 ◽  
Keyword(s):  
Cyclic Amp ◽  
Vasoactive Intestinal Peptide ◽  
Xenopus Oocytes ◽  
Membrane Conductance ◽  
Phosphodiesterase Inhibitors ◽  
Membrane Currents ◽  
Follicular Cells ◽  
Ionic Basis ◽  
Cyclase Activator ◽  
Adenylate Cyclase Activator

Membrane currents were recorded from voltage-clamped Xenopus laevis oocytes, surrounded by their enveloping follicular and epithelial cells. Porcine vasoactive intestinal peptide (VIP) generated a membrane current due to an increase in membrane conductance to K + . The VIP current was mimicked by the adenylate cyclase activator forskolin and was potentiated by phosphodiesterase inhibitors, suggesting that adenosine 3', 5'-cyclic monophosphate (cyclic AMP) plays a role in mediating the response. Though resembling the follicle’s responses to catecholamines and adenosine in ionic basis and apparent mechanism, the response to VIP was not blocked by catecholaminergic or purinergic antagonists, indicating the presence of a specific VIP receptor in the follicle. Among the VIP related peptides, PHM-27 generated similar but smaller K + currents and porcine secretin and glucagon neither elicited a response nor blocked that to VIP. After treating follicles with collagenase to remove the epithelial and follicular cells the responses to VIP were either substantially reduced or abolished, suggesting that the VIP receptors and K + channels are both located in the follicular cells.

Changes in intracellular calcium and in membrane currents evoked by injection of inositol trisphosphate into Xenopus oocytes

1986 ◽  
Vol 228 (1252) ◽  
pp. 307-316 ◽  
Keyword(s):  
Xenopus Laevis ◽  
Calcium Channels ◽  
Intracellular Calcium ◽  
Xenopus Oocytes ◽  
Inositol Trisphosphate ◽  
Surface Membrane ◽  
Extracellular Calcium ◽  
Membrane Currents ◽  
Free Solution ◽  
Calcium Signals

Intracellular calcium was monitored by the use of aequorin in voltage-clamped oocytes of Xenopus laevis . Injection of inositol trisphosphate (IP 3 ) into oocytes elicited slowly rising and decaying aequorin/calcium signals and produced oscillatory chloride membrane currents. These responses did not depend upon extracellular calcium, since they could be elicited in calcium-free solution and after addition of cobalt or lanthanum to block calcium channels in the surface membrane. We conclude that IP 3 causes the release of calcium from intracellular stores in the oocyte. Injections of calcium gave aequorin and membrane current responses that were more transient than those seen with IP 3 .

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