The ATAF1 transcription factor is a key regulator of aldehyde dehydrogenase 7B4 (ALDH7B4) gene expression in Arabidopsis thaliana

Plant heat stress transcription factors (HSFs) are involved in the response to heat. In Arabidopsis thaliana the HSFs genes are completely identified, however there was no information available about these genes in Vasconcellea pubescens (Chamburo) until now. In this preliminary work we describe the VPHSFB1 gene of V. pubescens (gene expression evaluated by RT-PCR and the partial sequence) that was induced by the increment of temperature. From our results, VPHSFB1 could be used as a heat response marker gene in tropical species. Key words: Caricaceae, gene expression, heat.

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Transcription Factor ANAC074 Binds to NRS1, NRS2, or MybSt1 Element in Addition to the NACRS to Regulate Gene Expression

International Journal of Molecular Sciences ◽

10.3390/ijms19103271 ◽

2018 ◽

Vol 19 (10) ◽

pp. 3271

Author(s):

Lin He ◽

Jingyu Xu ◽

Yucheng Wang ◽

Kejun Yang

Keyword(s):

Gene Expression ◽

Arabidopsis Thaliana ◽

Transcription Factor ◽

Transcription Factors ◽

Biological Processes ◽

Regulate Gene Expression ◽

Cis Element ◽

Stress Responsive Genes ◽

Regulate Gene ◽

Gus Assays

NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in many biological processes, and mainly bind to the NACRS with core sequences “CACG” or “CATGTG” to regulate gene expression. However, whether NAC proteins can bind to other motifs without these core sequences remains unknown. In this study, we employed a Transcription Factor-Centered Yeast one Hybrid (TF-Centered Y1H) screen to study the motifs recognized by ANAC074. In addition to the NACRS core cis-element, we identified that ANAC074 could bind to MybSt1, NRS1, and NRS2. Y1H and GUS assays showed that ANAC074 could bind the promoters of ethylene responsive genes and stress responsive genes via the NRS1, NRS2, or MybSt1 element. ChIP study further confirmed that the bindings of ANAC074 to MybSt1, NRS1, and NRS2 actually occurred in Arabidopsis. Furthermore, ten NAC proteins from different NAC subfamilies in Arabidopsis thaliana were selected and confirmed to bind to the MybSt1, NRS1, and NRS2 motifs, indicating that they are recognized commonly by NACs. These findings will help us to further reveal the functions of NAC proteins.

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Copper microRNAs govern the formation of giant feeding cells induced by the root knot nematode Meloidogyne incognita in Arabidopsis thaliana

10.1101/2021.10.25.465754 ◽

2021 ◽

Author(s):

Stéphanie Jaubert ◽

Yara Noureddine ◽

Bruno FAVERY ◽

Michaël QUENTIN ◽

Pierre Abad ◽

...

Keyword(s):

Gene Expression ◽

Arabidopsis Thaliana ◽

Transcription Factor ◽

Solanum Lycopersicum ◽

Meloidogyne Incognita ◽

Essential Role ◽

Signaling Cascade ◽

Root Knot Nematode ◽

Root Cells

miR408 and miR398 are two conserved microRNAs which expression is activated by the SPL7 transcription factor in response to copper starvation. We identified these two microRNAs families as upregulated in Arabidopsis thaliana and Solanum lycopersicum roots infected by root-knot nematodes. These endoparasites induce the dedifferentiation of a few root cells and the reprogramming of their gene expression to generate giant feeding cells. By combining functional approaches, we deciphered the signaling cascade involving these microRNAs, their regulator and their targets. MIR408 expression was located within nematode-induced feeding cells in which it co-localised with SPL7 expression and was regulated by copper. Moreover, infection assays with mir408 and spl7 KO mutants or lines expressing targets rendered resistant to cleavage by miR398 demonstrated the essential role of the SPL7/MIR408/MIR398 module in the formation of giant feeding cells. Our findings reveals how perturbation of plant copper homeostasis, via the SPL7/MIR408/MIR398 module, governs the formation of nematode-induced feeding cells.

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