Regulatory role of microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase for shikonin biosynthesis in Lithospermum erythrorhizon cell suspension cultures

Planta ◽  
1998 ◽  
Vol 204 (2) ◽  
pp. 234-241 ◽  
Author(s):  
B. Markus Lange ◽  
Klaus Severin ◽  
Andreas Bechthold ◽  
Lutz Heide
Keyword(s):  
Cell Suspension ◽  
Coenzyme A ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regulatory Role ◽  
Lithospermum Erythrorhizon ◽  
Coenzyme A Reductase

Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon

1987 ◽  
Vol 6 (3) ◽  
pp. 197-199 ◽  
Author(s):  
Hiroshi Deno ◽  
Chuzo Suga ◽  
Teijiro Morimoto ◽  
Yasuhiro Fujita
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Lithospermum Erythrorhizon ◽  
Shikonin Derivatives

Biosynthese von Cyanidin in Zellsuspensionskulturen von Haplopappus gracilis / Biosynthesis of Cyanidin in Cell Suspension Cultures of Haplopappus gracilis

1971 ◽  
Vol 26 (6) ◽  
pp. 581-585 ◽  
Author(s):  
Hansjörg Fritsch ◽  
Klaus Hahlbrock ◽  
Hans Grisebach
Keyword(s):  
Cell Suspension ◽  
Phenylalanine Ammonia Lyase ◽  
Cell Suspension Cultures ◽  
Maximal Activity ◽  
Suspension Cultures ◽  
Feeding Time ◽  
Haplopappus Gracilis ◽  
Time Required ◽  
Anthocyanin Production

Earlier work with buckwheat seedlings showed that dihydrokaempferol (3,5,7,4′-tetrahydroxyflavanone) (1) is a good precursor for cyanidin and quercetin. Since cell suspension cultures have several advantages for the quantitative comparison of the incorporation of a radioactive precursor into a product, the role of 1 in the biosynthesis of cyanidin was reinvestigated with cell cultures of Haplopappus gracilis. These cultures show good production of cyanidin-glycosides in blue light in a medium containing sucrose, 2,4-D and Fe-EDTA. Maximum anthocyanin production and maximal activity of phenylalanine ammonia-lyase is reached about 60 — 70 hrs. after the onset of illumination. The incorporation of 1 and of phenylalanine and 4,2′,4′,6′-tetrahydroxychalcone into cyanidin was compared under identical conditions after 1, 3 and 6 hrs. feeding time. 1 prooved to be by far the best precursor for cyanidin. The feeding time required was much shorter and the incorporation rates were much higher than in experiments with seedlings.

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