Concordance of the histological diagnosis of type 1 autoimmune pancreatitis and its distinction from pancreatic ductal adenocarcinoma with endoscopic ultrasound-guided fine needle biopsy specimens: an interobserver agreement study

2021 ◽  
Author(s):  
Kenji Notohara ◽  
Terumi Kamisawa ◽  
Toru Furukawa ◽  
Noriyoshi Fukushima ◽  
Takeshi Uehara ◽  
...  
Keyword(s):  
Needle Biopsy ◽  
Interobserver Agreement ◽  
Fine Needle Biopsy ◽  
Ductal Adenocarcinoma ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Biopsy Specimens ◽  
Type 1 Autoimmune Pancreatitis ◽  
Agreement Study

scholarly journals Endoscopic Ultrasound-Guided Fine-Needle Biopsy As A Valuable Tool for Studying The Intra-Tumoral Microbiome in Pancreatic Ductal Adenocarcinoma

2021 ◽  
Author(s):  
Chia-Sheng Chu ◽  
Chi-Ying Yang ◽  
Chun-Chieh Yeh ◽  
Ro-Ting Lin ◽  
Chi-Ching Chen ◽  
...  
Keyword(s):  
Needle Biopsy ◽  
Alpha Diversity ◽  
Fine Needle Biopsy ◽  
Ductal Adenocarcinoma ◽  
Cancer Tissue ◽  
Ultrasound Guided ◽  
Pancreatic Cancer Tissue ◽  
Surgical Biopsy ◽  
Fine Needle ◽  
Microbiome Profile

Abstract Introduction: A new approach by investigating the intra-tumoral microbiome raised great interest because they may influence the host immune response and natural history of the disease. However, previous studies on the intra-tumoral microbiome of pancreatic ductal adenocarcinoma (PDAC) were mostly based on examining the formalin-fixed paraffin-embedded tumor specimens. This study aims to investigate the feasibility of using endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) as a complementary procedure of surgical biopsy to obtain adequate fresh pancreatic cancer tissue for intra-tumoral microbial research. Materials and methods: This was a prospective pilot study performed at a single tertiary referral center. We obtained pancreatic cancer tissue by EUS-FNB and surgical biopsy, respectively. We amplified the V3-V4 hyper-variable region of bacterial 16S ribosomal ribonucleic acid (rRNA) genes, constructed a pair-end library, and performed high-throughput sequencing. Results: From August 2020 to November 2020, nine eligible patients with PDAC were enrolled in this study. The intra-tumoral microbiome profile was successfully generated from the PDAC cancer tissue obtained by EUS-FNB as well as by surgical biopsy. There was no significant difference in intra-tumoral alpha-diversity or bacterial taxonomic composition between tissues obtained by EUS-FNB and by surgical biopsy. Conclusion: EUS-FNB can collect sufficient fresh cancer tissue for microbiome analyses without complication. The intra-tumoral microbiome profile in tissues obtained by EUS-FNB had similar alpha-diversity and taxonomic profiles with those obtained by surgical biopsy. It implicated, except for surgical biopsy, EUS-FNB can be another valid and valuable tool for studying intra-tumoral microbiome in patients with resectable and unresectable PDAC.

Electron microscopy of ultrasound-guided fine-needle biopsy specimens

1987 ◽  
Vol 60 (712) ◽  
pp. 351-353 ◽  
Author(s):  
Maxwell Sehested ◽  
Niels Juul ◽  
Bo Hainau ◽  
Søren Torp-Pedersen
Keyword(s):  
Electron Microscopy ◽  
Needle Biopsy ◽  
Fine Needle Biopsy ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Biopsy Specimens

scholarly journals Utility of endoscopic ultrasound-guided fine-needle biopsy in the diagnosis of type 1 autoimmune pancreatitis

2020 ◽  
Vol 08 (12) ◽  
pp. E1855-E1861
Author(s):  
Kofi W. Oppong ◽  
Pardeep Maheshwari ◽  
Manu K. Nayar ◽  
Antony Darne ◽  
Daniel Parkinson ◽  
...  
Keyword(s):  
Endoscopic Ultrasound ◽  
Autoimmune Pancreatitis ◽  
Needle Biopsy ◽  
Final Diagnosis ◽  
Fine Needle Biopsy ◽  
Pathological Feature ◽  
International Consensus ◽  
Fine Needle ◽  
Type 1 Autoimmune Pancreatitis

Abstract Background and study aims Endoscopic ultrasound (EUS)-guided fine-needle aspiration (FNA) performs poorly in the histological diagnosis of type 1 autoimmune pancreatitis (AIP). The aim of this study was to assess the performance of fine-needle biopsy (FNB) comparing reverse bevel (RB) and fork-tip (FT) needles. Patients and methods A retrospective study of prospectively maintained databases was performed. Patients with a final diagnosis of type 1 AIP who underwent EUS-FNB during diagnostic workup were included. Pathology reports were reviewed and classified as per international consensus diagnostic criteria (ICDC). The Primary outcome was EUS-FNB sensitivity in diagnosing type 1 AIP. Results Between March 2011 and December 2018, 24 patients with a final diagnosis of type 1 AIP underwent FNB. Six patients underwent biopsy with the RB needle and 18 with the FT needle. Mean age (± SD) 62.2 (± 11.4), 17 (70.8 %) male. No RB samples were diagnostic compared to 14 (78 %) FT; P = 0.001; of which 13 (72 %) were level 1. In eight (44 %) of FT cases a diagnosis was not possible without histology. Initial biopsy was diagnostic in five (62.5 %) of these cases. Including repeat biopsy, seven (87 %) had a diagnosis made by FT needle. Obliterative phlebitis (44 %) was the least frequently identified pathological feature and immunoglobulin (IgG)4 + plasma cells > 10 per high power field (78 %) the most common. Conclusion The FT needle demonstrated good performance for diagnosing type 1 AIP. The results support the preferential use of this core biopsy needle for EUS pancreatic tissue sampling.

scholarly journals Endoscopic ultrasound-guided fine-needle biopsy as a tool for studying the intra-tumoral microbiome in pancreatic ductal adenocarcinoma: a pilot study

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Chia-Sheng Chu ◽  
Chi-Ying Yang ◽  
Chun-Chieh Yeh ◽  
Ro-Ting Lin ◽  
Chi-Ching Chen ◽  
...  
Keyword(s):  
Needle Biopsy ◽  
Alpha Diversity ◽  
Fine Needle Biopsy ◽  
Ductal Adenocarcinoma ◽  
Cancer Tissue ◽  
Ultrasound Guided ◽  
Pancreatic Cancer Tissue ◽  
Surgical Biopsy ◽  
Fine Needle ◽  
Microbiome Profile

AbstractA new approach by investigating the intra-tumoral microbiome raised great interest because they may influence the host immune response and natural history of the disease. However, previous studies on the intra-tumoral microbiome of pancreatic ductal adenocarcinoma (PDAC) were mostly based on examining the formalin-fixed paraffin-embedded tumor specimens. This study aims to investigate the feasibility of using endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) as a complementary procedure of surgical biopsy to obtain adequate fresh pancreatic cancer tissue for intra-tumoral microbial research. This was a prospective pilot study performed at a single tertiary referral center. We obtained pancreatic cancer tissue by EUS-FNB and surgical biopsy, respectively. We amplified the V3-V4 hyper-variable region of bacterial 16S ribosomal ribonucleic acid (rRNA) genes, constructed a pair-end library, and performed high-throughput sequencing. From August 2020 to November 2020, nine eligible patients with PDAC were enrolled in this study. The intra-tumoral microbiome profile was successfully generated from the PDAC cancer tissue obtained by EUS-FNB as well as by surgical biopsy. There was no significant difference in intra-tumoral alpha-diversity or bacterial taxonomic composition between tissues obtained by EUS-FNB and by surgical biopsy. EUS-FNB can collect sufficient fresh cancer tissue for microbiome analyses without complication. The intra-tumoral microbiome profile in tissues obtained by EUS-FNB had similar alpha-diversity and taxonomic profiles with those obtained by surgical biopsy. It implicated, except for surgical biopsy, EUS-FNB can be another valid and valuable tool for studying intra-tumoral microbiome in patients with resectable and unresectable PDAC.

scholarly journals Accurate Prognosis Prediction of Pancreatic Ductal Adenocarcinoma Using Integrated Clinico-Genomic Data of Endoscopic Ultrasound-Guided Fine Needle Biopsy

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2791
Author(s):  
Joo Kyung Park ◽  
Hyemin Kim ◽  
Dae-Soon Son ◽  
Nayoung K. D. Kim ◽  
Young Kyung Sung ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Copy Number ◽  
Needle Biopsy ◽  
Fine Needle Biopsy ◽  
Ductal Adenocarcinoma ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Prognosis Prediction ◽  
Targeted Deep Sequencing ◽  
Genomic Model

The aim of this study was to investigate the clinical utility of minimal specimens acquired from endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) and perform targeted deep sequencing as a prognosis prediction tool for pancreatic ductal adenocarcinoma (PDAC). A total of 116 specimens with pathologically confirmed PDAC via EUS-FNB were tested using CancerSCAN® panel for a customized targeted deep sequencing. Clinical prognostic factors significantly associated with survival in PDACs were as follows: stage, tumor mass size, tumor location, metastasis, chemotherapy, and initial CA19-9 level. A total of 114 patients (98.3%) had at least a single genetic alteration, and no mutations were detected in two patients, although they were qualified for the targeted deep sequencing. The frequencies of major gene mutations responsible for PDACs were KRAS 90%, CDKN2A 31%, TP53 77%, and SMAD4 29%. A somatic point mutation of NF1, copy number alteration of SMAD4, and loss-of-function of CDKN2A were significantly associated genetic factors for overall survival. Moreover, BRCA2 point mutation was related to liver metastasis. Finally, a clinico-genomic model was developed to estimate the prognosis of patients with PDAC based on clinical parameters and genetic alterations affecting survival in patients; 20 single nucleotide variants and three copy number variations were selected. Targeted deep sequencing on minimal specimens of PDACs was performed, and it was applied to establish a clinico-genomic model for prognosis prediction.

Pancreatic actinomycosis treated by antibiotics after diagnosis using endoscopic ultrasound-guided fine-needle biopsy

2021 ◽  
Author(s):  
Kazuya Miyamoto ◽  
Kazuyuki Matsumoto ◽  
Kazuki Ocho ◽  
Koji Fujita ◽  
Shigeru Horiguchi ◽  
...  
Keyword(s):  
Endoscopic Ultrasound ◽  
Needle Biopsy ◽  
Fine Needle Biopsy ◽  
Ultrasound Guided ◽  
Fine Needle
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