A novel histologic grading system based on lymphovascular invasion, perineural invasion, and tumor budding in colorectal cancer

Abstract Background: No.253 lymph nodes metastasis is related to poor prognosis of colorectal cancer, while the role of No.253 lymph nodes dissection in colorectal cancer is still controversial.Methods: A total of 157 patients who were received laparoscopic radical resection of colorectal cancer with preservation of the left colon artery + D3 lymph node dissection (low ligation + high dissection) in our hospital were enrolled. No.253 lymph nodes of each patients were dissected and sent for examination. Clinicaopathological factors correlated with No.253 lymph nodes metastasis, including gender, age, tumor location, tumor size, length of tumor from the anus, whether lymphovascular invasion, whether perineural invasion, tumor markers CEA, CA125 and CA199, tumor T stage, whether it is distant metastasis, the total number of lymph nodes harvested, tumor type, and histologic grade were respectively analyzed.Results: A total of 2286 lymph nodes were sent for examination, of which 557 No.253 lymph nodes were sent for examination. Among them, 5 patients had a total of 27 No.253 lymph node metastases. Preoperative CA125 level (X2=4.736, p=0.030), whether perineural invasion (X2=8.086, p<0.01), whether lymphovascular invasion (X2= 7.053, p<0.01), tumor type (X2=21.019, p<0.01), histologic grade (X2=15.315, p<0.01) were significantly correlated with positive No.253 lymph nodes metastasis. A multivariate logistic regression analysis showed that none of risk factor from above are independent risk factors for No.253 lymph node metastasis(P>0.05).Conclusion: Preoperative CA125 level, whether perineural invasion, whether lymphovascular invasion, tumor type, histologic grade were risk factors of the No.253 lymph nodes metastasis. Multivariate analysis showed that five risk factors from above were not the independent risk factor for No.253 lymph nodes metastasis. Colorectal cancer patients with the risk factors above were suggested to receive radical resection + D3 lymph node dissection.

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Association Between Tumor Budding Grade and T Stage in Predicting Recurrence in High-risk Stage Ii Colorectal Cancer

10.21203/rs.3.rs-284704/v1 ◽

2021 ◽

Author(s):

Hiroyuki Kodama ◽

Toshifumi Yamaguchi ◽

Yasunobu Ishizuka ◽

Masahiko Aoki ◽

Takahiro Miyamoto ◽

...

Keyword(s):

Colorectal Cancer ◽

High Risk ◽

Perineural Invasion ◽

Tumor Location ◽

Tumor Budding ◽

Free Survival ◽

T Stage ◽

Stage Ii Colorectal Cancer ◽

The Relationship ◽

Prognostic Association

Abstract In pathological stage (pStage) II colon cancer, factors such as T stage (T) are high-risk features (HRF) for recurrence. The SACURA trial showed that tumor budding (BD) grade was also associated with recurrence: the 5-year recurrence-free survival (RFS) rate was lower in patients in the BD3 group compared with that of other groups. Interestingly, the BD3 group had a higher proportion of patients with T4. We investigated the prognostic association between T4 and BD3 for recurrence in pStage II colorectal cancer (pII-CRC) with HRF. We analyzed pII-CRC patients with HRF between 2013–2018 at our hospital, Japan. Inclusion criteria were as follows: ≥1 HRF [<12 lymph nodes examined (<12LN), lymph/vascular-invasion, perineural-invasion, T, BD, and histologic-type]. We primarily analyzed the relationship between each factor and RFS. Among 2,920 pII-CRC patients, 448 had HRF. Of these, 43 (9.6%) had T4 and 236 (52.7%) had BD3. On initial analysis, <12LN (P=0.0412), tumor location (P=0.0023), T4 (P=0.0023), and BD3 (P=0.0396) were independent prognostic factors for RFS. Among 257 patients with BD3 and/or T4, the 5-year RFS rates were 81.3%, 74.6%, and 66.2% for patients with BD3 (214 patients), T4 (22 patients), and BD3 plus T4 (21 patients), respectively (hazard ratio 3.08, P=0.0079). pII-CRC patients with BD3 plus T4 had poorer prognosis than those with other factors.

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S100 Staining Adds to the Prognostic Significance of the Combination of Perineural Invasion and Lymphovascular Invasion in Colorectal Cancer

Applied Immunohistochemistry & Molecular Morphology ◽

10.1097/pai.0000000000000733 ◽

2020 ◽

Vol 28 (5) ◽

pp. 354-359 ◽

Cited By ~ 1

Author(s):

Gabriella A. Conte ◽

Omar Qari ◽

Genevieve A. Fasano ◽

Robyn K. Guinto ◽

Laura Palo ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymphovascular Invasion ◽

Perineural Invasion ◽

Prognostic Significance

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Poorly differentiated clusters and tumor budding are important prognostic factors in colorectal carcinomas

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2021.6110 ◽

2021 ◽

Author(s):

Aura Jurescu ◽

Alis Dema ◽

Adrian Văduva ◽

Adelina Gheju ◽

Octavia Vița ◽

...

Keyword(s):

Prognostic Factors ◽

Lymphovascular Invasion ◽

Tumor Stage ◽

Nodal Status ◽

Tumor Budding ◽

Grading System ◽

Strongly Correlated ◽

Colorectal Carcinomas ◽

Poorly Differentiated Clusters ◽

Poorly Differentiated

The aim of our study was to assess the prognostic value of the two new grading systems based on the quantification of tumor budding - TB (GBd) and poorly differentiated clusters - PDCs (PDCs-G) in colorectal carcinomas (CRC). We performed a retrospective study on 71 CRC patients who underwent surgery at the Emergency County Hospital, Timișoara. CRC cases were classified based on haematoxylin-eosin slides, using the conventional grading system, GBd and PDCs-G, respectively. We used two-tier and three-tier grading schemes for each system. Subsequently, we evaluated associations with other prognostic factors in CRC. Based on the three-tier GBd (GBd-3t) most cases (34/69, 49.27%) were classified as G3Bd-3t, while based on the conventional grading system, the majority of the cases (55/69, 79.71%) were considered G2. On the other hand, based on the three-tier PDCs-G system (PDCs-G-3t), most cases (31/69, 44.93%) were PDCs-G2-3t. We also noted a more significant association of GBd-3t with other prognostic parameters analyzed, as compared to the conventional grading system. Nodal status, tumor stage, and lymphovascular invasion were strongly correlated with GBd-3t (p=0.0001). Furthermore, we noted that PDCs-G-3t correlated more significantly than the conventional grading system with nodal status (p<0.0001), tumor stage (p=0.0003), lymphovascular invasion (p<0.0001), perineural invasion (p=0.005) and the tumor border configuration (p<0.0001). High GBd and PDCs-G grades correlate directly with other negative prognostic factors in CRC.Thus, these new parameters/classification methods could be used as additional tools for risk stratification in patients with CRC.

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The relationship between tumor budding and survival in colorectal carcinomas

Revista da Associação Médica Brasileira ◽

10.1590/1806-9282.65.12.1442 ◽

2019 ◽

Vol 65 (12) ◽

pp. 1442-1447 ◽

Cited By ~ 1

Author(s):

Songul Peltek Ozer ◽

Saime Gul Barut ◽

Bahri Ozer ◽

Oguz Catal ◽

Mustafa Sit

Keyword(s):

Prognostic Factors ◽

Lymphovascular Invasion ◽

Perineural Invasion ◽

Histological Grade ◽

Prognostic Significance ◽

Tumor Budding ◽

Colorectal Carcinomas ◽

Pathology Reports ◽

Lymph Node Stage ◽

The Relationship

SUMMARY OBJECTIVE Tumor budding is a parameter that is increasingly understood in colorectal carcinomas. We aimed to investigate the relationship between tumor budding, prognostic factors, and survival METHODS A total of 185 patients who had undergone colorectal surgery were observed. Tumor budding, the tumor budding score, and the relationship between these and prognostic factors, and survival investigated. RESULTS Tumor budding was found in 91 (49.2%) cases. The relationship between the tumor budding score and histological grade, lymphovascular invasion, perineural invasion, pathological lymph node stage, and mortality rates were significant. CONCLUSION In our study, the relationship between tumor budding and survival is very strong. Considering these findings and the literature, the prognostic significance of tumor budding becomes clear and should be stated in pathology reports.

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Correction to: Lymphovascular invasion, perineural invasion, and tumor budding are prognostic factors for stage I colon cancer recurrence

International Journal of Colorectal Disease ◽

10.1007/s00384-020-03573-3 ◽

2020 ◽

Vol 35 (6) ◽

pp. 1171-1171

Author(s):

Seijong Kim ◽

Jung Wook Huh ◽

Woo Yong Lee ◽

Seong Hyeon Yun ◽

Hee Cheol Kim ◽

...

Keyword(s):

Colon Cancer ◽

Prognostic Factors ◽

Lymphovascular Invasion ◽

Perineural Invasion ◽

Cancer Recurrence ◽

Stage I ◽

Tumor Budding ◽

Colon Cancer Recurrence

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Refining the ITBCC tumor budding scoring system with a “zero-budding” category in colorectal cancer

Virchows Archiv ◽

10.1007/s00428-021-03090-w ◽

2021 ◽

Author(s):

Inti Zlobec ◽

Melanie Bächli ◽

Francesca Galuppini ◽

Martin D. Berger ◽

Heather E. Dawson ◽

...

Keyword(s):

Colorectal Cancer ◽

Prognostic Factors ◽

Clinical Relevance ◽

Perineural Invasion ◽

Tumor Grade ◽

Consensus Conference ◽

Clinicopathological Features ◽

Tumor Budding ◽

Additional Information ◽

Significant Difference

AbstractTumor budding scoring guidelines from the International Tumor Budding Consensus Conference (ITBCC) for colorectal cancer propose three groups: BD1 (0–4 buds/0.785 mm2), BD2 (5–9 buds/0.785 mm2), and BD3 (10 or more buds/0.785 mm2). Here, we investigate whether a fourth scoring category, namely zero buds, may have additional clinical relevance. The number of tumor buds/0.785 mm2 was scored in 959 cases. Those with zero tumor buds were considered BD0, while a new BD1 category of 1–4 buds was proposed. Associations of both scoring approaches with clinicopathological features were analyzed. Conventional ITBCC scoring showed expected associations with unfavorable histopathological prognostic factors. In total, 111/959 (11.6%) were BD0. A significant difference was found when BD0 was compared statistically to BD1 (1–4 buds) for pT, TNM, tumor grade, and lymphatic, venous, and perineural invasion (p < 0.01, all). Tumors with BD0 occur relatively frequently and contribute additional information on tumor behavior. BD0 should be considered for subsequent ITBCC guidelines.

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