The use of a recombinant baculovirus expressing a chitinase from the hard tick Haemaphysalis longicornis and its potential application as a bioacaricide for tick control

2005 ◽  
Vol 98 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Severine P. Assenga ◽  
Myungjo You ◽  
Chee Huey Shy ◽  
Junya Yamagishi ◽  
Takeshi Sakaguchi ◽  
...  
Keyword(s):  
Potential Application ◽  
Recombinant Baculovirus ◽  
Tick Control ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

scholarly journals Bacterial Microbiota Analysis Demonstrates That Tick Can Acquire Bacteria From Habitat and Host Blood Meal

2021 ◽  
Author(s):  
Si-Si Li ◽  
Xiao-Yu Zhang ◽  
Xue-Jiao Zhou ◽  
Kai-Li Chen ◽  
Abolfazl Masoudi ◽  
...  
Keyword(s):  
Life Cycle ◽  
High Throughput Sequencing ◽  
Developmental Stages ◽  
Tick Control ◽  
Hard Tick ◽  
Haemaphysalis Longicornis ◽  
Bacterial Microbiota ◽  
Bacterial Richness ◽  
Host Blood ◽  
Blood Meals

Abstract Exploring the bacterial microbiota is imperative to tick control since it has an important role in tick physiology and vector capacity. The life cycle of ticks consists of parasitic and non-parasitic stages, with a diversity of habitats and host blood meals. Whether and how these factors, such as tick developmental stages, tick organs, habitats and host blood meals affect tick bacterial microbiota is poorly elucidated. In the present study, we investigated the bacterial microbiotas of hard tick Haemaphysalis longicornis, their blood meals and habitats using 16S rRNA high-throughput sequencing. The bacterial richness and diversity in ticks varied depending on the tick developmental stage, feeding status and the tick organs. Results showed that fed ticks present a higher bacterial richness suggesting that ticks may acquire bacteria from blood meals. The significant overlap of the bacteriota of fed ticks and the host blood also support this possibility. Another possibility is that blood meals can stimulate the proliferation of certain bacteria. However, most shared bacteria cannot transmit throughout the tick life cycle, as they were not present in tick eggs. The most shared bacteria between ticks and habitats are genus of Staphylococcus, Pseudomonus, Enterobacter, Acinetobacer and Stenotrophomonas, some of them are also present in tick organ, suggesting that these environmental bacteria cannot be completely washed away and can be acquired by ticks. As tick reproductive organ, ovary showed the lowest bacterial richness and diversity compared to other organs. The predominant proportion of Coxiella in fed females and ovary further demonstrated that this genus is required for H. longicornis reproduction system. These findings further reveal that the bacterial composition of ticks is influenced by a variety of factors and will help in subsequent studies of the function of these bacteria.

Hemolymph defensin from the hard tick Haemaphysalis longicornis attacks Gram-positive bacteria

2018 ◽  
Vol 156 ◽  
pp. 14-18 ◽  
Author(s):  
Yurika Yada ◽  
Melbourne Rio Talactac ◽  
Kodai Kusakisako ◽  
Emmanuel Pacia Hernandez ◽  
Remil Linggatong Galay ◽  
...  
Keyword(s):  
Hard Tick ◽  
Haemaphysalis Longicornis ◽  
Gram Positive ◽  
Gram Positive Bacteria

RNAi of the translation inhibition gene 4E-BP identified from the hard tick, Haemaphysalis longicornis, affects lipid storage during the off-host starvation period of ticks

2012 ◽  
Vol 111 (2) ◽  
pp. 889-896 ◽  
Author(s):  
Aiko Kume ◽  
Damdinsuren Boldbaatar ◽  
Yuko Takazawa ◽  
Rika Umemiya-Shirafuji ◽  
Tetsuya Tanaka ◽  
...  
Keyword(s):  
Lipid Storage ◽  
Hard Tick ◽  
Haemaphysalis Longicornis ◽  
Starvation Period ◽  
Translation Inhibition

scholarly journals Interactive Gene Expression Between Metarhizium anisopliae JEF-290 and Longhorned Tick Haemaphysalis longicornis at Early Stage of Infection

2021 ◽  
Vol 12 ◽  
Author(s):  
Mi Rong Lee ◽  
Jong Cheol Kim ◽  
So Eun Park ◽  
Se Jin Lee ◽  
Woo Jin Kim ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Early Stage ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Transcriptional Level ◽  
Hard Tick ◽  
Haemaphysalis Longicornis ◽  
Gene Set Enrichment ◽  
Gene Set ◽  
Number Of Patients

The longhorned tick, Haemaphysalis longicornis (Acari: Ixodidae), is a hard tick and a vector for severe fever with thrombocytopenia syndrome (SFTS) virus. The number of patients infected with SFTS is rapidly increasing. Recently, the invertebrate pathogen Metarhizium anisopliae JEF-290 was reported to be useful to control the tick as an alternative to chemical acaricides, which are not easily applicable in human living areas where the tick is widely spread. In this study, we analyzed how the tick and the fungal pathogen interact at the transcriptional level. Field-collected tick nymphs were treated with JEF-290 conidia at 1 × 108 conidia/ml. In the early stage of infection with 2.5% mortality, the infected ticks were subjected to RNA sequencing, and non-infected ticks and fungal masses served as controls. Fungus and tick genes were mostly up-regulated at the early stage of infection. In the gene set enrichment analysis of the infecting fungus, catabolic processes that included lipids, phospholipids, and detoxification processes, the response to oxidative stress, and toxic substances were significantly up-regulated. In this fungal up-regulation, various lipase, antioxidant enzyme, and hydrolase genes were highly transcribed. The gene set enrichment analysis of the infected tick showed that many peptide synthesis processes including translation, peptide metabolism, ribonucleotide metabolism, and energy production processes that included ATP generation and ADP metabolism were significantly up-regulated. Structurally, mitochondria and ribosome subunit genes in ticks were highly transcribed to upregulate these processes. Together these results indicate that JEF-290 initiates process that infects the tick while the tick actively defends against the fungal attack. This work provides background to improve our understanding of the early stage of fungal infection in longhorned tick.

Molecular cloning and functional characterization of an aspartic protease from the hard tick Haemaphysalis longicornis

2006 ◽  
Vol 36 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Damdinsuren Boldbaatar ◽  
Chummy Sikalizyo Sikasunge ◽  
Badgar Battsetseg ◽  
Xuenan Xuan ◽  
Kozo Fujisaki
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
Aspartic Protease ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

Cloning and molecular characterization of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis

2004 ◽  
Vol 34 (8) ◽  
pp. 799-808 ◽  
Author(s):  
Takeharu Miyoshi ◽  
Naotoshi Tsuji ◽  
M Khyrul Islam ◽  
Tsugihiko Kamio ◽  
Kozo Fujisaki
Keyword(s):  
Molecular Characterization ◽  
Serine Proteinase ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

Assessment of potential effects and detection efficacy of a fluorescent marking system on a medically important hard tick, Haemaphysalis longicornis (Acari: Ixodidae)

2019 ◽  
Vol 75 (10) ◽  
pp. 2735-2743 ◽  
Author(s):  
Joo‐Young Kim ◽  
Jung‐Wook Kho ◽  
Minhyung Jung ◽  
Doo‐Hyung Lee
Keyword(s):  
Hard Tick ◽  
Haemaphysalis Longicornis

scholarly journals Enzymatic Characterization of a Cubilin-Related Serine Proteinase from the Hard Tick Haemaphysalis longicornis

2004 ◽  
Vol 66 (10) ◽  
pp. 1195-1198 ◽  
Author(s):  
Takeharu MIYOSHI ◽  
Naotoshi TSUJI ◽  
M. Khyrul ISLAM ◽  
Tsugihiko KAMIO ◽  
Kozo FUJISAKI
Keyword(s):  
Serine Proteinase ◽  
Hard Tick ◽  
Haemaphysalis Longicornis ◽  
Enzymatic Characterization
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