Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana

2010 ◽  
Vol 108 (6) ◽  
pp. 1551-1556 ◽  
Author(s):  
Jing Cui ◽  
Nan Li ◽  
Zhong Quan Wang ◽  
Peng Jiang ◽  
Xi Meng Lin
Keyword(s):  
Spirometra Mansoni ◽  
Human Sparganosis

Virus-Like Particles in Spirometra Sp. from a Case of Human Sparganosis

1975 ◽  
Vol 33 ◽  
pp. 648-649
Author(s):  
C.N. Sun ◽  
J.J. Daly ◽  
H.J. White ◽  
A.L. Barron
Keyword(s):  
Osmium Tetroxide ◽  
The United States ◽  
Virus Like Particles ◽  
Virus Particles ◽  
Human Host ◽  
Spirometra Mansoni ◽  
Outer Aspect ◽  
Animal Hosts ◽  
The Right ◽  
Human Sparganosis

Mueller and Strano have reported finding virus-like particles lining the walls of excretory ducts in plerocercoid (sparganum) larvae of the pseudophyllidean tapeworms, Sparganum proliferum, from a human host, and Spirometra mansonoides and Spirometra mansoni, from animal hosts. Although human sparganosis caused by these species is distributed world-wide, only 50 cases have been officially reported in the United States. A sparganum in a nodule from the outer aspect of the right thigh of a 30 year-old male in Arkansas has recently been described which is believed to be either S. mansonoides or S. mansoni. Since the previously studied material of these two species were from non-human hosts, this worm afforded the opportunity to examine a non-proliferating sparganum from a human host for the presence of virus particles.The worm, which was 90 mm long and 1 mm wide, was fixed in neutral buffered formalin and post-fixed with buffered 2% osmium tetroxide.

Some aspects of human sparganosis in Korea

1975 ◽  
Vol 13 (1) ◽  
pp. 60 ◽  
Author(s):  
Seung Yull Cho ◽  
Jong Hoa Bae ◽  
Byong Seol Seo
Keyword(s):  
Human Sparganosis

Cleavage of immunoglobulin G by excretory–secretory cathepsin S-like protease of Spirometra mansoni plerocercoid

Parasitology ◽  
1994 ◽  
Vol 109 (5) ◽  
pp. 611-621 ◽  
Author(s):  
Y. Kong ◽  
Y.-B. Chung ◽  
S.-Y. Cho ◽  
S.-Y. Kang
Keyword(s):  
Immunoglobulin G ◽  
Cathepsin L ◽  
Immunoblot Analysis ◽  
Cathepsin S ◽  
Terminal Amino Acid ◽  
Optimal Activity ◽  
Spirometra Mansoni ◽  
Terminal Amino ◽  
Secretory Products ◽  
Ph Range

When immunoglobulin G (IgG) was incubated with Spirometra mansoni plerocercoid (sparganum), it was cleaved into Fab and Fc fragments. Fab/c fragments were also hydrolysed. The digestion was accelerated by dithiothreitol (DTT), indicating that cleavage of IgG heavy chain was due to a cysteine protease secreted into the medium. The responsible enzyme, of Mr 27 (± 0·8) kDa, was purified by a series of thiopropyl affinity, Sephacryl S-300 HR and DEAE-anion exchange chromatographies, either from worm extracts or from excretory–secretory products (ESP). The purified, thiol-dependent protease showed an optimal activity at pH 5·7 with 0·1 M sodium acetate but was active over the pH range 4·5–8·0. Its activity was inhibited completely by 10−5 M L-trans-epoxysuccinylleucylamido(4-guanidino) butane (E-64) and 1 mM iodoacetamide (IAA), but by only 53% using the specific cathepsin L inhibitor, Z-Phe-Phe-CHN2 (5 × 10−5 M). Partial NH2-terminal amino acid sequence was Leu-Pro-Asp-Ser-Val-Asn-Trp-Arg-Glu-Gly-Ala-Val-Thr-Ala-Val which showed 80% homology to human cathepsin S. Immunoblot analysis showed that sera from infected patients exhibited IgE antibody reaction. It is proposed that cleavage of immunoglobulin by an excreted–secreted, cathepsin S-like, allergenic protease is a mechanism of immune evasion used by the sparganum.

A case of mammary sparganosis due to infection with Spirometra mansoni

2019 ◽  
Vol 25 (4) ◽  
pp. 712-715
Author(s):  
Jun Zuo ◽  
Hai Gang Feng ◽  
Sha Sha Xie ◽  
Jun Tao Yang
Keyword(s):  
Spirometra Mansoni

Molecular characterization of a Spirometra mansoni antigenic polypeptide gene encoding a 28.7 kDa protein

2014 ◽  
Vol 113 (9) ◽  
pp. 3511-3516 ◽  
Author(s):  
Jing Cui ◽  
Tong Wei ◽  
Li Na Liu ◽  
Xi Zhang ◽  
Xin Qi ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gene Encoding ◽  
Spirometra Mansoni

A case of Human sparganosis from California

1964 ◽  
Vol 37 (3) ◽  
pp. 491-494 ◽  
Author(s):  
Edward K Markell ◽  
Seth L Haber
Keyword(s):  
Human Sparganosis

scholarly journals Primer hallazgo de Spirometra mansoni en Felis domesticus de Costa Rica

2004 ◽  
Vol 59 (3-4) ◽  
Author(s):  
IDALIA VALERIO ◽  
BEATRÍZ RODRÍGUEZ ◽  
MISAEL CHINCHILLA
Keyword(s):  
Costa Rica ◽  
Spirometra Mansoni ◽  
Felis Domesticus

Platynosomum fastosum-induced chronic intrahepatic cholangitis and Spirometra spp. infections in feral cats from Grand Cayman

2011 ◽  
Vol 86 (2) ◽  
pp. 209-214 ◽  
Author(s):  
S.A. Headley ◽  
M.A. Gillen ◽  
A.W.D. Sanches ◽  
M.Z. Satti
Keyword(s):  
Bile Ducts ◽  
Inflammatory Cells ◽  
Cayman Islands ◽  
Feral Cats ◽  
Grand Cayman ◽  
Intrahepatic Bile Ducts ◽  
Sporadic Cases ◽  
Bile Duct Epithelium ◽  
Liver Fragments ◽  
Human Sparganosis

AbstractThe occurrence of platynosomiasis and intestinal sparganosis is described in feral cats from Grand Cayman, Cayman Islands. Spirometra spp. was observed within the intestine of 18.18% (10/55) of cats; 1.18% (1/55) of cats demonstrated gross and histological manifestation of parasitism by Platynosomum fastosum, but 14.5% (8/55) of cats had the characteristic pathological manifestations of P. fastosum-induced intrahepatic cholangitis without the concomitant presence of the intraductal trematode. Combined parasitism (Spirometra spp. and P. fastosum) was observed in 9.09% (5/55) of feral cats. Significant pathological findings were only associated with the hepatic fluke, P. fastosum, and were grossly characterized by moderate hepatomegaly with enlarged and dilated bile ducts. Examples of cestodes with morphological features characteristic of Spirometra spp. were observed within the small intestine without any associated pathological lesion. The histopathological evaluation of liver fragments revealed chronic intrahepatic cholangitis with and without the associated intraductal trematode, and was characterized by marked periductal fibrosis, adenomatous proliferation of bile duct epithelium, dilation of intrahepatic bile ducts and portal accumulations of inflammatory cells. The occurrence of the cestode in feral cats coupled with factors that are unique to Grand Cayman makes this island the ideal location for sporadic cases of human sparganosis.

scholarly journals Spirometra(Pseudophyllidea, Diphyllobothriidae) Severely Infecting Wild-Caught Snakes from Food Markets in Guangzhou and Shenzhen, Guangdong, China: Implications for Public Health

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Fumin Wang ◽  
Weiye Li ◽  
Liushuai Hua ◽  
Shiping Gong ◽  
Jiajie Xiao ◽  
...  
Keyword(s):  
Public Health ◽  
Subcutaneous Tissue ◽  
Food Habit ◽  
Cooking Methods ◽  
Muscular Tissue ◽  
Intermediate Hosts ◽  
Food Markets ◽  
Public Health Agencies ◽  
Important Intermediate ◽  
Human Sparganosis

Sparganosis is a zoonotic disease caused by the spargana ofSpirometra, and snake is one of the important intermediate hosts of spargana. In some areas of China, snake is regarded as popular delicious food, and such a food habit potentially increases the prevalence of human sparganosis. To understand the prevalence ofSpirometrain snakes in food markets, we conducted a study in two representative cities (Guangzhou and Shenzhen), during January–August 2013. A total of 456 snakes of 13 species were examined and 251 individuals of 10 species were infected bySpirometra, accounting for 55.0% of the total samples. The worm burden per infected snake ranged from 1 to 213, and the prevalence in the 13 species was 0∼96.2%. More than half (58.1%) of the spargana were located in muscular tissue, 25.6% in subcutaneous tissue, and 16.3% in coelomic cavity. The results indicated thatSpirometraseverely infected snakes in food markets in Guangzhou and Shenzhen, implying that eating snakes has great health risk and improper cooking methods may increase the risk ofSpirometrainfection in humans in China. Additional steps should be considered by the governments and public health agencies to prevent the risk of snake-associatedSpirometrainfections in humans.

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